Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08775 and RR42_RS08780 are separated by 91 nucleotides RR42_RS08780 and RR42_RS08785 overlap by 4 nucleotides RR42_RS08785 and RR42_RS08790 are separated by 157 nucleotides
RR42_RS08775: RR42_RS08775 - protease, at 1,839,781 to 1,840,734
_RS08775
RR42_RS08780: RR42_RS08780 - protease, at 1,840,826 to 1,841,743
_RS08780
RR42_RS08785: RR42_RS08785 - lipid carrier protein, at 1,841,740 to 1,842,192
_RS08785
RR42_RS08790: RR42_RS08790 - zinc-binding protein, at 1,842,350 to 1,842,799
_RS08790
Position (kb)
1840
1841
1842 Strain fitness (log2 ratio)
-1
0
1 at 1839.936 kb on + strand, within RR42_RS08775 at 1839.937 kb on - strand, within RR42_RS08775 at 1839.937 kb on - strand, within RR42_RS08775 at 1839.937 kb on - strand, within RR42_RS08775 at 1840.143 kb on - strand, within RR42_RS08775 at 1840.279 kb on + strand, within RR42_RS08775 at 1840.397 kb on - strand, within RR42_RS08775 at 1840.468 kb on + strand, within RR42_RS08775 at 1840.468 kb on + strand, within RR42_RS08775 at 1840.469 kb on - strand, within RR42_RS08775 at 1840.469 kb on - strand, within RR42_RS08775 at 1840.577 kb on - strand, within RR42_RS08775 at 1840.627 kb on - strand, within RR42_RS08775 at 1840.715 kb on - strand at 1840.810 kb on - strand at 1840.810 kb on - strand at 1840.887 kb on - strand at 1840.887 kb on - strand at 1840.889 kb on + strand at 1840.919 kb on + strand, within RR42_RS08780 at 1840.920 kb on - strand, within RR42_RS08780 at 1840.920 kb on - strand, within RR42_RS08780 at 1840.955 kb on + strand, within RR42_RS08780 at 1841.207 kb on + strand, within RR42_RS08780 at 1841.207 kb on + strand, within RR42_RS08780 at 1841.219 kb on - strand, within RR42_RS08780 at 1842.104 kb on - strand, within RR42_RS08785 at 1842.234 kb on + strand at 1842.235 kb on - strand at 1842.235 kb on - strand at 1842.235 kb on - strand at 1842.300 kb on + strand at 1842.390 kb on - strand at 1842.433 kb on + strand, within RR42_RS08790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 1,839,936 + RR42_RS08775 0.16 -0.5 1,839,937 - RR42_RS08775 0.16 -0.2 1,839,937 - RR42_RS08775 0.16 -0.5 1,839,937 - RR42_RS08775 0.16 +0.7 1,840,143 - RR42_RS08775 0.38 +0.4 1,840,279 + RR42_RS08775 0.52 +0.5 1,840,397 - RR42_RS08775 0.65 +0.2 1,840,468 + RR42_RS08775 0.72 -0.4 1,840,468 + RR42_RS08775 0.72 +1.1 1,840,469 - RR42_RS08775 0.72 +0.1 1,840,469 - RR42_RS08775 0.72 -0.2 1,840,577 - RR42_RS08775 0.83 -0.6 1,840,627 - RR42_RS08775 0.89 -0.1 1,840,715 - -0.2 1,840,810 - +0.2 1,840,810 - -0.1 1,840,887 - -0.1 1,840,887 - -1.2 1,840,889 + +0.1 1,840,919 + RR42_RS08780 0.10 +0.7 1,840,920 - RR42_RS08780 0.10 -0.5 1,840,920 - RR42_RS08780 0.10 +0.0 1,840,955 + RR42_RS08780 0.14 -0.1 1,841,207 + RR42_RS08780 0.42 -1.0 1,841,207 + RR42_RS08780 0.42 -0.7 1,841,219 - RR42_RS08780 0.43 -0.7 1,842,104 - RR42_RS08785 0.80 -0.3 1,842,234 + +0.1 1,842,235 - -0.0 1,842,235 - +0.9 1,842,235 - +0.1 1,842,300 + -0.3 1,842,390 - +0.4 1,842,433 + RR42_RS08790 0.18 +0.3
Or see this region's nucleotide sequence