Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08190 and RR42_RS08195 are separated by 238 nucleotides RR42_RS08195 and RR42_RS08200 overlap by 4 nucleotides RR42_RS08200 and RR42_RS08205 overlap by 4 nucleotides RR42_RS08205 and RR42_RS08210 are separated by 347 nucleotides RR42_RS08210 and RR42_RS08215 are separated by 101 nucleotides
RR42_RS08190: RR42_RS08190 - hypothetical protein, at 1,741,454 to 1,741,774
_RS08190
RR42_RS08195: RR42_RS08195 - hypothetical protein, at 1,742,013 to 1,742,531
_RS08195
RR42_RS08200: RR42_RS08200 - hypothetical protein, at 1,742,528 to 1,742,743
_RS08200
RR42_RS08205: RR42_RS08205 - hypothetical protein, at 1,742,740 to 1,743,105
_RS08205
RR42_RS08210: RR42_RS08210 - hypothetical protein, at 1,743,453 to 1,743,794
_RS08210
RR42_RS08215: RR42_RS08215 - DNA-binding protein, at 1,743,896 to 1,744,501
_RS08215
Position (kb) 1742
1743
1744 Strain fitness (log2 ratio) -2
-1
0
1 at 1741.755 kb on - strand at 1741.958 kb on + strand at 1743.187 kb on - strand at 1743.412 kb on - strand at 1743.754 kb on - strand, within RR42_RS08210 at 1743.812 kb on - strand at 1743.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 1,741,755 - -0.5 1,741,958 + -1.9 1,743,187 - +0.9 1,743,412 - +0.4 1,743,754 - RR42_RS08210 0.88 -1.1 1,743,812 - +0.0 1,743,917 + -1.8
Or see this region's nucleotide sequence