Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04390 and RR42_RS04395 are separated by 167 nucleotides RR42_RS04395 and RR42_RS04400 are separated by 30 nucleotides RR42_RS04400 and RR42_RS04405 are separated by 143 nucleotides
RR42_RS04390: RR42_RS04390 - ABC transporter, at 977,045 to 977,965
_RS04390
RR42_RS04395: RR42_RS04395 - cysteine ABC transporter substrate-binding protein, at 978,133 to 978,930
_RS04395
RR42_RS04400: RR42_RS04400 - ABC transporter permease, at 978,961 to 979,620
_RS04400
RR42_RS04405: RR42_RS04405 - cyanophycin synthetase, at 979,764 to 982,334
_RS04405
Position (kb)
978
979
980 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 977.964 kb on - strand at 978.035 kb on + strand at 978.132 kb on + strand at 978.132 kb on + strand at 978.133 kb on - strand at 978.133 kb on - strand at 978.133 kb on - strand at 978.230 kb on + strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.272 kb on - strand, within RR42_RS04395 at 978.287 kb on - strand, within RR42_RS04395 at 978.490 kb on + strand, within RR42_RS04395 at 978.490 kb on + strand, within RR42_RS04395 at 978.491 kb on - strand, within RR42_RS04395 at 978.497 kb on - strand, within RR42_RS04395 at 978.598 kb on + strand, within RR42_RS04395 at 978.599 kb on - strand, within RR42_RS04395 at 978.599 kb on - strand, within RR42_RS04395 at 978.661 kb on + strand, within RR42_RS04395 at 979.130 kb on - strand, within RR42_RS04400 at 979.180 kb on + strand, within RR42_RS04400 at 979.180 kb on + strand, within RR42_RS04400 at 979.181 kb on - strand, within RR42_RS04400 at 979.181 kb on - strand, within RR42_RS04400 at 979.184 kb on - strand, within RR42_RS04400 at 979.261 kb on + strand, within RR42_RS04400 at 979.262 kb on - strand, within RR42_RS04400 at 979.325 kb on - strand, within RR42_RS04400 at 979.325 kb on - strand, within RR42_RS04400 at 979.360 kb on + strand, within RR42_RS04400 at 979.361 kb on - strand, within RR42_RS04400 at 979.504 kb on + strand, within RR42_RS04400 at 979.522 kb on + strand, within RR42_RS04400 at 979.523 kb on - strand, within RR42_RS04400 at 979.539 kb on + strand, within RR42_RS04400 at 979.540 kb on - strand, within RR42_RS04400 at 979.540 kb on - strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.543 kb on + strand, within RR42_RS04400 at 979.572 kb on + strand at 979.632 kb on + strand at 979.632 kb on + strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.633 kb on - strand at 979.644 kb on + strand at 979.644 kb on + strand at 979.790 kb on + strand at 979.866 kb on - strand at 979.991 kb on - strand at 979.991 kb on - strand at 980.180 kb on - strand, within RR42_RS04405 at 980.188 kb on - strand, within RR42_RS04405 at 980.188 kb on - strand, within RR42_RS04405 at 980.442 kb on - strand, within RR42_RS04405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 977,964 - +0.1 978,035 + +0.1 978,132 + +0.3 978,132 + +0.4 978,133 - +0.2 978,133 - +0.0 978,133 - +0.5 978,230 + RR42_RS04395 0.12 -0.3 978,272 - RR42_RS04395 0.17 -0.6 978,272 - RR42_RS04395 0.17 +0.3 978,272 - RR42_RS04395 0.17 +1.4 978,272 - RR42_RS04395 0.17 -0.8 978,287 - RR42_RS04395 0.19 +1.8 978,490 + RR42_RS04395 0.45 -0.7 978,490 + RR42_RS04395 0.45 +0.9 978,491 - RR42_RS04395 0.45 +0.2 978,497 - RR42_RS04395 0.46 +0.3 978,598 + RR42_RS04395 0.58 +0.0 978,599 - RR42_RS04395 0.58 -0.8 978,599 - RR42_RS04395 0.58 +0.9 978,661 + RR42_RS04395 0.66 -1.2 979,130 - RR42_RS04400 0.26 +1.3 979,180 + RR42_RS04400 0.33 -0.5 979,180 + RR42_RS04400 0.33 +0.2 979,181 - RR42_RS04400 0.33 +0.1 979,181 - RR42_RS04400 0.33 +0.1 979,184 - RR42_RS04400 0.34 +0.8 979,261 + RR42_RS04400 0.45 -0.6 979,262 - RR42_RS04400 0.46 -1.1 979,325 - RR42_RS04400 0.55 -0.0 979,325 - RR42_RS04400 0.55 +0.1 979,360 + RR42_RS04400 0.60 -0.2 979,361 - RR42_RS04400 0.61 +0.5 979,504 + RR42_RS04400 0.82 +0.8 979,522 + RR42_RS04400 0.85 -0.3 979,523 - RR42_RS04400 0.85 +0.5 979,539 + RR42_RS04400 0.88 +0.3 979,540 - RR42_RS04400 0.88 -0.6 979,540 - RR42_RS04400 0.88 +0.7 979,543 + RR42_RS04400 0.88 +0.1 979,543 + RR42_RS04400 0.88 -0.8 979,543 + RR42_RS04400 0.88 +1.3 979,572 + -1.2 979,632 + -0.1 979,632 + +1.1 979,633 - +0.8 979,633 - +0.3 979,633 - -1.1 979,633 - +0.7 979,633 - -0.4 979,644 + +0.3 979,644 + +0.6 979,790 + -1.1 979,866 - -0.7 979,991 - -0.2 979,991 - +0.5 980,180 - RR42_RS04405 0.16 -2.5 980,188 - RR42_RS04405 0.16 -0.2 980,188 - RR42_RS04405 0.16 -3.0 980,442 - RR42_RS04405 0.26 -1.0
Or see this region's nucleotide sequence