Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04345 and RR42_RS04350 are separated by 243 nucleotides RR42_RS04350 and RR42_RS04355 are separated by 116 nucleotides
RR42_RS04345: RR42_RS04345 - iron ABC transporter permease, at 964,983 to 966,611
_RS04345
RR42_RS04350: RR42_RS04350 - ABC transporter ATP-binding protein, at 966,855 to 967,916
_RS04350
RR42_RS04355: RR42_RS04355 - recombination factor protein RarA, at 968,033 to 969,400
_RS04355
Position (kb) 966
967
968 Strain fitness (log2 ratio) -2
-1
0
1
2 at 965.947 kb on + strand, within RR42_RS04345 at 965.980 kb on + strand, within RR42_RS04345 at 966.169 kb on + strand, within RR42_RS04345 at 966.169 kb on + strand, within RR42_RS04345 at 966.308 kb on + strand, within RR42_RS04345 at 966.311 kb on - strand, within RR42_RS04345 at 966.329 kb on - strand, within RR42_RS04345 at 966.329 kb on - strand, within RR42_RS04345 at 966.391 kb on + strand, within RR42_RS04345 at 966.391 kb on + strand, within RR42_RS04345 at 966.467 kb on + strand at 966.467 kb on + strand at 966.467 kb on + strand at 966.468 kb on - strand at 966.478 kb on + strand at 966.479 kb on - strand at 966.657 kb on + strand at 966.657 kb on + strand at 966.658 kb on - strand at 966.713 kb on - strand at 966.751 kb on - strand at 966.762 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.813 kb on - strand at 966.813 kb on - strand at 966.814 kb on + strand at 966.814 kb on + strand at 966.814 kb on + strand at 966.815 kb on - strand at 966.815 kb on - strand at 967.132 kb on - strand, within RR42_RS04350 at 967.132 kb on - strand, within RR42_RS04350 at 967.532 kb on + strand, within RR42_RS04350 at 967.532 kb on + strand, within RR42_RS04350 at 967.539 kb on + strand, within RR42_RS04350 at 967.649 kb on + strand, within RR42_RS04350 at 967.649 kb on + strand, within RR42_RS04350 at 967.650 kb on - strand, within RR42_RS04350 at 967.650 kb on - strand, within RR42_RS04350 at 967.789 kb on - strand, within RR42_RS04350 at 967.949 kb on + strand at 967.949 kb on + strand at 967.950 kb on - strand at 967.950 kb on - strand at 967.950 kb on - strand at 968.005 kb on + strand at 968.006 kb on - strand at 968.017 kb on + strand at 968.325 kb on - strand, within RR42_RS04355 at 968.502 kb on - strand, within RR42_RS04355 at 968.502 kb on - strand, within RR42_RS04355 at 968.765 kb on + strand, within RR42_RS04355 at 968.765 kb on + strand, within RR42_RS04355 at 968.825 kb on + strand, within RR42_RS04355 at 968.825 kb on + strand, within RR42_RS04355 at 968.826 kb on - strand, within RR42_RS04355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 965,947 + RR42_RS04345 0.59 -1.1 965,980 + RR42_RS04345 0.61 -1.4 966,169 + RR42_RS04345 0.73 -1.4 966,169 + RR42_RS04345 0.73 -0.2 966,308 + RR42_RS04345 0.81 +0.1 966,311 - RR42_RS04345 0.82 +1.1 966,329 - RR42_RS04345 0.83 +0.2 966,329 - RR42_RS04345 0.83 +0.5 966,391 + RR42_RS04345 0.86 +0.1 966,391 + RR42_RS04345 0.86 -0.4 966,467 + -0.5 966,467 + -1.0 966,467 + -0.1 966,468 - +0.1 966,478 + -0.4 966,479 - -1.7 966,657 + -0.1 966,657 + -0.7 966,658 - +0.2 966,713 - +0.2 966,751 - -0.1 966,762 + -0.6 966,812 + +0.4 966,812 + -0.9 966,812 + +0.3 966,812 + +0.1 966,812 + -0.1 966,813 - -0.7 966,813 - -0.0 966,814 + -0.3 966,814 + +2.1 966,814 + +0.5 966,815 - -0.5 966,815 - +1.8 967,132 - RR42_RS04350 0.26 -2.3 967,132 - RR42_RS04350 0.26 -1.4 967,532 + RR42_RS04350 0.64 +0.3 967,532 + RR42_RS04350 0.64 -0.2 967,539 + RR42_RS04350 0.64 +1.1 967,649 + RR42_RS04350 0.75 -0.1 967,649 + RR42_RS04350 0.75 -1.5 967,650 - RR42_RS04350 0.75 -1.4 967,650 - RR42_RS04350 0.75 -0.7 967,789 - RR42_RS04350 0.88 -0.2 967,949 + +0.5 967,949 + -0.1 967,950 - +0.3 967,950 - -0.4 967,950 - +0.1 968,005 + -0.0 968,006 - +0.5 968,017 + +0.3 968,325 - RR42_RS04355 0.21 +0.8 968,502 - RR42_RS04355 0.34 -0.8 968,502 - RR42_RS04355 0.34 -0.3 968,765 + RR42_RS04355 0.54 +0.1 968,765 + RR42_RS04355 0.54 -0.5 968,825 + RR42_RS04355 0.58 +0.5 968,825 + RR42_RS04355 0.58 -0.5 968,826 - RR42_RS04355 0.58 +0.1
Or see this region's nucleotide sequence