Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04205 and RR42_RS04210 are separated by 173 nucleotides RR42_RS04210 and RR42_RS04215 overlap by 11 nucleotides RR42_RS04215 and RR42_RS04220 are separated by 6 nucleotides
RR42_RS04205: RR42_RS04205 - glycine cleavage system regulatory protein, at 930,956 to 931,843
_RS04205
RR42_RS04210: RR42_RS04210 - membrane protein, at 932,017 to 933,306
_RS04210
RR42_RS04215: RR42_RS04215 - hypothetical protein, at 933,296 to 933,799
_RS04215
RR42_RS04220: RR42_RS04220 - membrane protein, at 933,806 to 934,666
_RS04220
Position (kb)
932
933
934 Strain fitness (log2 ratio)
-2
-1
0
1 at 931.032 kb on + strand at 931.033 kb on - strand at 931.033 kb on - strand at 931.093 kb on - strand, within RR42_RS04205 at 931.093 kb on - strand, within RR42_RS04205 at 931.093 kb on - strand, within RR42_RS04205 at 931.411 kb on - strand, within RR42_RS04205 at 931.954 kb on - strand at 931.972 kb on + strand at 932.033 kb on + strand at 932.033 kb on + strand at 932.033 kb on + strand at 932.146 kb on + strand, within RR42_RS04210 at 932.146 kb on + strand, within RR42_RS04210 at 932.146 kb on + strand, within RR42_RS04210 at 932.147 kb on - strand, within RR42_RS04210 at 932.147 kb on - strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.149 kb on - strand, within RR42_RS04210 at 932.239 kb on + strand, within RR42_RS04210 at 932.239 kb on + strand, within RR42_RS04210 at 932.240 kb on - strand, within RR42_RS04210 at 932.352 kb on + strand, within RR42_RS04210 at 932.581 kb on + strand, within RR42_RS04210 at 932.581 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 933.019 kb on + strand, within RR42_RS04210 at 933.020 kb on - strand, within RR42_RS04210 at 933.265 kb on + strand at 933.265 kb on + strand at 933.266 kb on - strand at 933.266 kb on - strand at 933.266 kb on - strand at 933.413 kb on + strand, within RR42_RS04215 at 933.445 kb on + strand, within RR42_RS04215 at 933.446 kb on - strand, within RR42_RS04215 at 933.446 kb on - strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 934.185 kb on + strand, within RR42_RS04220 at 934.186 kb on - strand, within RR42_RS04220
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 931,032 + +0.7 931,033 - -1.1 931,033 - -0.3 931,093 - RR42_RS04205 0.15 -1.1 931,093 - RR42_RS04205 0.15 -2.5 931,093 - RR42_RS04205 0.15 -0.2 931,411 - RR42_RS04205 0.51 +0.5 931,954 - -0.6 931,972 + -1.7 932,033 + +0.1 932,033 + -0.3 932,033 + +0.6 932,146 + RR42_RS04210 0.10 +0.1 932,146 + RR42_RS04210 0.10 -0.7 932,146 + RR42_RS04210 0.10 +0.1 932,147 - RR42_RS04210 0.10 +0.4 932,147 - RR42_RS04210 0.10 +0.6 932,148 + RR42_RS04210 0.10 -0.3 932,148 + RR42_RS04210 0.10 -0.2 932,148 + RR42_RS04210 0.10 +1.2 932,149 - RR42_RS04210 0.10 -0.2 932,239 + RR42_RS04210 0.17 +0.7 932,239 + RR42_RS04210 0.17 -0.4 932,240 - RR42_RS04210 0.17 +0.1 932,352 + RR42_RS04210 0.26 -0.3 932,581 + RR42_RS04210 0.44 -0.1 932,581 + RR42_RS04210 0.44 -0.1 932,809 + RR42_RS04210 0.61 -1.5 932,809 + RR42_RS04210 0.61 +0.1 932,923 + RR42_RS04210 0.70 +0.5 932,923 + RR42_RS04210 0.70 -0.2 932,923 + RR42_RS04210 0.70 -0.8 932,923 + RR42_RS04210 0.70 -1.9 932,924 - RR42_RS04210 0.70 -0.1 932,924 - RR42_RS04210 0.70 -0.1 932,924 - RR42_RS04210 0.70 -0.1 932,924 - RR42_RS04210 0.70 -0.9 933,019 + RR42_RS04210 0.78 -0.5 933,020 - RR42_RS04210 0.78 +0.2 933,265 + -0.7 933,265 + -0.6 933,266 - +0.5 933,266 - +1.1 933,266 - -1.3 933,413 + RR42_RS04215 0.23 +0.1 933,445 + RR42_RS04215 0.30 +0.8 933,446 - RR42_RS04215 0.30 -0.3 933,446 - RR42_RS04215 0.30 +0.3 933,532 + RR42_RS04215 0.47 -0.4 933,532 + RR42_RS04215 0.47 -1.8 933,532 + RR42_RS04215 0.47 -0.1 933,532 + RR42_RS04215 0.47 +0.1 933,533 - RR42_RS04215 0.47 -0.0 933,533 - RR42_RS04215 0.47 -0.2 933,533 - RR42_RS04215 0.47 -0.4 933,533 - RR42_RS04215 0.47 -0.5 933,533 - RR42_RS04215 0.47 -0.0 933,534 + RR42_RS04215 0.47 -1.1 933,534 + RR42_RS04215 0.47 +0.0 933,534 + RR42_RS04215 0.47 -0.1 933,534 + RR42_RS04215 0.47 +0.3 933,535 - RR42_RS04215 0.47 +0.6 933,535 - RR42_RS04215 0.47 -0.3 933,535 - RR42_RS04215 0.47 -0.4 933,535 - RR42_RS04215 0.47 -0.7 933,535 - RR42_RS04215 0.47 -0.7 934,185 + RR42_RS04220 0.44 -2.2 934,186 - RR42_RS04220 0.44 -0.3
Or see this region's nucleotide sequence