Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03320 and RR42_RS03325 overlap by 4 nucleotides RR42_RS03325 and RR42_RS03330 are separated by 211 nucleotides RR42_RS03330 and RR42_RS03335 are separated by 64 nucleotides
RR42_RS03320: RR42_RS03320 - transcriptional regulator, at 739,742 to 740,473
_RS03320
RR42_RS03325: RR42_RS03325 - membrane protein, at 740,470 to 741,747
_RS03325
RR42_RS03330: RR42_RS03330 - GYD family protein, at 741,959 to 742,252
_RS03330
RR42_RS03335: RR42_RS03335 - glycerol-3-phosphate acyltransferase, at 742,317 to 742,934
_RS03335
Position (kb) 740
741
742 Strain fitness (log2 ratio) -1
0
1
2 at 739.481 kb on + strand at 739.481 kb on + strand at 739.482 kb on - strand at 739.482 kb on - strand at 739.482 kb on - strand at 739.482 kb on - strand at 739.489 kb on - strand at 739.601 kb on + strand at 739.601 kb on + strand at 739.602 kb on - strand at 739.612 kb on + strand at 739.612 kb on + strand at 739.612 kb on + strand at 739.613 kb on - strand at 739.613 kb on - strand at 739.668 kb on + strand at 739.686 kb on - strand at 739.726 kb on + strand at 739.727 kb on - strand at 739.730 kb on + strand at 739.730 kb on + strand at 739.730 kb on + strand at 739.730 kb on + strand at 739.731 kb on - strand at 739.731 kb on - strand at 739.741 kb on + strand at 739.742 kb on - strand at 740.051 kb on + strand, within RR42_RS03320 at 740.051 kb on + strand, within RR42_RS03320 at 740.052 kb on - strand, within RR42_RS03320 at 740.313 kb on - strand, within RR42_RS03320 at 740.313 kb on - strand, within RR42_RS03320 at 740.406 kb on - strand at 740.406 kb on - strand at 740.434 kb on + strand at 740.434 kb on + strand at 740.435 kb on - strand at 740.435 kb on - strand at 740.435 kb on - strand at 740.786 kb on + strand, within RR42_RS03325 at 740.927 kb on + strand, within RR42_RS03325 at 740.927 kb on + strand, within RR42_RS03325 at 741.124 kb on - strand, within RR42_RS03325 at 741.452 kb on - strand, within RR42_RS03325 at 741.452 kb on - strand, within RR42_RS03325 at 741.452 kb on - strand, within RR42_RS03325 at 741.457 kb on + strand, within RR42_RS03325 at 741.458 kb on - strand at 741.843 kb on - strand at 741.843 kb on - strand at 741.864 kb on + strand at 741.882 kb on - strand at 741.899 kb on + strand at 741.900 kb on - strand at 741.982 kb on + strand at 741.982 kb on + strand at 741.982 kb on + strand at 741.983 kb on - strand at 742.103 kb on + strand, within RR42_RS03330 at 742.104 kb on - strand, within RR42_RS03330 at 742.104 kb on - strand, within RR42_RS03330 at 742.104 kb on - strand, within RR42_RS03330 at 742.114 kb on - strand, within RR42_RS03330 at 742.124 kb on - strand, within RR42_RS03330 at 742.124 kb on - strand, within RR42_RS03330 at 742.250 kb on + strand at 742.251 kb on - strand at 742.254 kb on + strand at 742.254 kb on + strand at 742.254 kb on + strand at 742.254 kb on + strand at 742.254 kb on + strand at 742.254 kb on + strand at 742.255 kb on - strand at 742.255 kb on - strand at 742.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 739,481 + -0.5 739,481 + +0.7 739,482 - +1.0 739,482 - +0.3 739,482 - +0.4 739,482 - -0.4 739,489 - +0.2 739,601 + +0.7 739,601 + +0.8 739,602 - +0.4 739,612 + +0.1 739,612 + +1.1 739,612 + -0.3 739,613 - +0.5 739,613 - -0.2 739,668 + +0.2 739,686 - +0.6 739,726 + +1.3 739,727 - +0.2 739,730 + +2.4 739,730 + -0.1 739,730 + +1.0 739,730 + +0.1 739,731 - +0.0 739,731 - -0.5 739,741 + +1.4 739,742 - -0.6 740,051 + RR42_RS03320 0.42 +0.5 740,051 + RR42_RS03320 0.42 +0.6 740,052 - RR42_RS03320 0.42 +0.8 740,313 - RR42_RS03320 0.78 -0.3 740,313 - RR42_RS03320 0.78 -0.6 740,406 - -0.2 740,406 - -0.2 740,434 + +0.8 740,434 + -0.6 740,435 - -0.5 740,435 - +0.8 740,435 - +0.8 740,786 + RR42_RS03325 0.25 +0.3 740,927 + RR42_RS03325 0.36 +0.4 740,927 + RR42_RS03325 0.36 +0.3 741,124 - RR42_RS03325 0.51 +0.4 741,452 - RR42_RS03325 0.77 +0.3 741,452 - RR42_RS03325 0.77 +1.0 741,452 - RR42_RS03325 0.77 +0.1 741,457 + RR42_RS03325 0.77 +0.7 741,458 - +2.1 741,843 - +0.3 741,843 - +0.1 741,864 + +0.2 741,882 - +0.9 741,899 + +0.2 741,900 - +0.9 741,982 + +0.3 741,982 + +0.6 741,982 + -0.1 741,983 - -0.5 742,103 + RR42_RS03330 0.49 -0.2 742,104 - RR42_RS03330 0.49 +1.0 742,104 - RR42_RS03330 0.49 +0.7 742,104 - RR42_RS03330 0.49 +0.6 742,114 - RR42_RS03330 0.53 +0.2 742,124 - RR42_RS03330 0.56 +1.2 742,124 - RR42_RS03330 0.56 -0.3 742,250 + +0.1 742,251 - +0.8 742,254 + +0.8 742,254 + +0.6 742,254 + -0.1 742,254 + +0.8 742,254 + +0.8 742,254 + +1.6 742,255 - +0.8 742,255 - -0.1 742,255 - +1.7
Or see this region's nucleotide sequence