Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03140 and RR42_RS03145 are separated by 94 nucleotides RR42_RS03145 and RR42_RS03150 are separated by 48 nucleotides RR42_RS03150 and RR42_RS03155 are separated by 77 nucleotides
RR42_RS03140: RR42_RS03140 - molybdenum cofactor biosynthesis protein MoaC, at 694,065 to 694,547
_RS03140
RR42_RS03145: RR42_RS03145 - peptidase M48, at 694,642 to 696,450
_RS03145
RR42_RS03150: RR42_RS03150 - hypothetical protein, at 696,499 to 697,110
_RS03150
RR42_RS03155: RR42_RS03155 - alpha/beta hydrolase, at 697,188 to 698,174
_RS03155
Position (kb) 694
695
696
697 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 693.661 kb on - strand at 693.684 kb on + strand at 693.685 kb on - strand at 693.723 kb on - strand at 693.799 kb on + strand at 693.799 kb on + strand at 693.800 kb on - strand at 693.800 kb on - strand at 693.800 kb on - strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.933 kb on + strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.934 kb on - strand at 693.936 kb on + strand at 693.937 kb on - strand at 693.965 kb on + strand at 694.028 kb on - strand at 694.066 kb on + strand at 694.066 kb on + strand at 694.067 kb on - strand at 694.168 kb on + strand, within RR42_RS03140 at 694.168 kb on + strand, within RR42_RS03140 at 694.169 kb on - strand, within RR42_RS03140 at 694.169 kb on - strand, within RR42_RS03140 at 694.169 kb on - strand, within RR42_RS03140 at 694.285 kb on + strand, within RR42_RS03140 at 694.324 kb on - strand, within RR42_RS03140 at 694.383 kb on + strand, within RR42_RS03140 at 694.383 kb on + strand, within RR42_RS03140 at 694.383 kb on + strand, within RR42_RS03140 at 694.505 kb on + strand at 694.505 kb on + strand at 694.505 kb on + strand at 694.506 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.671 kb on + strand at 694.671 kb on + strand at 694.671 kb on + strand at 694.671 kb on + strand at 694.672 kb on - strand at 694.672 kb on - strand at 694.672 kb on - strand at 694.855 kb on - strand, within RR42_RS03145 at 694.855 kb on - strand, within RR42_RS03145 at 694.912 kb on + strand, within RR42_RS03145 at 694.912 kb on + strand, within RR42_RS03145 at 694.913 kb on - strand, within RR42_RS03145 at 694.913 kb on - strand, within RR42_RS03145 at 694.913 kb on - strand, within RR42_RS03145 at 695.104 kb on + strand, within RR42_RS03145 at 695.104 kb on + strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.105 kb on - strand, within RR42_RS03145 at 695.131 kb on + strand, within RR42_RS03145 at 695.132 kb on - strand, within RR42_RS03145 at 695.279 kb on - strand, within RR42_RS03145 at 695.280 kb on + strand, within RR42_RS03145 at 695.281 kb on - strand, within RR42_RS03145 at 695.377 kb on - strand, within RR42_RS03145 at 695.377 kb on - strand, within RR42_RS03145 at 695.668 kb on + strand, within RR42_RS03145 at 695.669 kb on - strand, within RR42_RS03145 at 695.765 kb on - strand, within RR42_RS03145 at 695.968 kb on + strand, within RR42_RS03145 at 695.969 kb on - strand, within RR42_RS03145 at 695.969 kb on - strand, within RR42_RS03145 at 695.969 kb on - strand, within RR42_RS03145 at 696.121 kb on + strand, within RR42_RS03145 at 696.122 kb on - strand, within RR42_RS03145 at 696.122 kb on - strand, within RR42_RS03145 at 696.236 kb on - strand, within RR42_RS03145 at 696.236 kb on - strand, within RR42_RS03145 at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.286 kb on + strand at 696.287 kb on - strand at 696.287 kb on - strand at 696.305 kb on - strand at 696.361 kb on + strand at 696.362 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.445 kb on - strand at 696.614 kb on - strand, within RR42_RS03150 at 696.663 kb on + strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.664 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.864 kb on - strand, within RR42_RS03150 at 696.905 kb on + strand, within RR42_RS03150 at 697.004 kb on + strand, within RR42_RS03150 at 697.005 kb on - strand, within RR42_RS03150 at 697.055 kb on + strand at 697.066 kb on + strand at 697.168 kb on - strand at 697.168 kb on - strand at 697.239 kb on - strand at 697.277 kb on + strand at 697.277 kb on + strand at 697.278 kb on - strand at 697.370 kb on - strand, within RR42_RS03155
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 693,661 - -1.4 693,684 + -0.4 693,685 - -1.3 693,723 - -1.1 693,799 + -2.0 693,799 + -0.1 693,800 - -1.1 693,800 - -0.3 693,800 - -1.3 693,933 + -0.7 693,933 + -0.9 693,933 + -0.3 693,933 + +0.1 693,933 + -0.9 693,933 + -0.6 693,933 + -0.8 693,934 - -1.8 693,934 - -0.5 693,934 - -0.8 693,934 - -1.2 693,934 - -1.5 693,936 + -0.7 693,937 - -0.1 693,965 + -0.8 694,028 - -0.6 694,066 + +0.3 694,066 + +0.2 694,067 - -0.5 694,168 + RR42_RS03140 0.21 +0.1 694,168 + RR42_RS03140 0.21 +0.0 694,169 - RR42_RS03140 0.22 -0.9 694,169 - RR42_RS03140 0.22 -0.9 694,169 - RR42_RS03140 0.22 +0.3 694,285 + RR42_RS03140 0.46 -0.5 694,324 - RR42_RS03140 0.54 -0.3 694,383 + RR42_RS03140 0.66 -1.5 694,383 + RR42_RS03140 0.66 +0.3 694,383 + RR42_RS03140 0.66 -0.3 694,505 + -0.6 694,505 + -0.2 694,505 + -1.1 694,506 - +0.0 694,667 - -0.5 694,667 - -1.1 694,667 - -0.6 694,671 + +0.5 694,671 + -0.8 694,671 + -0.1 694,671 + -1.0 694,672 - +0.3 694,672 - -1.4 694,672 - -1.6 694,855 - RR42_RS03145 0.12 -1.8 694,855 - RR42_RS03145 0.12 -1.0 694,912 + RR42_RS03145 0.15 -0.9 694,912 + RR42_RS03145 0.15 -0.8 694,913 - RR42_RS03145 0.15 -2.7 694,913 - RR42_RS03145 0.15 +0.0 694,913 - RR42_RS03145 0.15 -1.7 695,104 + RR42_RS03145 0.26 -2.6 695,104 + RR42_RS03145 0.26 -1.8 695,105 - RR42_RS03145 0.26 -1.3 695,105 - RR42_RS03145 0.26 -3.1 695,105 - RR42_RS03145 0.26 +0.3 695,105 - RR42_RS03145 0.26 -1.1 695,131 + RR42_RS03145 0.27 -1.2 695,132 - RR42_RS03145 0.27 -1.0 695,279 - RR42_RS03145 0.35 -0.5 695,280 + RR42_RS03145 0.35 -2.9 695,281 - RR42_RS03145 0.35 -2.1 695,377 - RR42_RS03145 0.41 +0.5 695,377 - RR42_RS03145 0.41 -0.8 695,668 + RR42_RS03145 0.57 -2.6 695,669 - RR42_RS03145 0.57 -1.0 695,765 - RR42_RS03145 0.62 -0.4 695,968 + RR42_RS03145 0.73 -1.2 695,969 - RR42_RS03145 0.73 -1.8 695,969 - RR42_RS03145 0.73 -1.0 695,969 - RR42_RS03145 0.73 -2.0 696,121 + RR42_RS03145 0.82 -1.1 696,122 - RR42_RS03145 0.82 -1.0 696,122 - RR42_RS03145 0.82 -0.2 696,236 - RR42_RS03145 0.88 -0.0 696,236 - RR42_RS03145 0.88 -2.0 696,286 + -0.9 696,286 + -0.4 696,286 + -0.2 696,286 + -2.2 696,286 + -0.8 696,287 - -2.2 696,287 - -2.0 696,305 - -1.5 696,361 + -0.2 696,362 - -1.4 696,445 - -0.1 696,445 - -0.5 696,445 - +0.1 696,614 - RR42_RS03150 0.19 -0.4 696,663 + RR42_RS03150 0.27 -0.6 696,664 - RR42_RS03150 0.27 +0.0 696,664 - RR42_RS03150 0.27 +0.2 696,864 - RR42_RS03150 0.60 +1.0 696,864 - RR42_RS03150 0.60 -0.5 696,905 + RR42_RS03150 0.66 +0.1 697,004 + RR42_RS03150 0.83 +0.3 697,005 - RR42_RS03150 0.83 -0.8 697,055 + -0.3 697,066 + +0.3 697,168 - -0.4 697,168 - +1.0 697,239 - +0.0 697,277 + -0.2 697,277 + -1.5 697,278 - +0.4 697,370 - RR42_RS03155 0.18 +0.5
Or see this region's nucleotide sequence