Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02950 and RR42_RS02955 overlap by 4 nucleotides RR42_RS02955 and RR42_RS02960 overlap by 1 nucleotides RR42_RS02960 and RR42_RS02965 overlap by 1 nucleotides
RR42_RS02950: RR42_RS02950 - malonate decarboxylase subunit beta, at 656,002 to 656,853
_RS02950
RR42_RS02955: RR42_RS02955 - malonate decarboxylase subunit delta, at 656,850 to 657,164
_RS02955
RR42_RS02960: RR42_RS02960 - triphosphoribosyl-dephospho-CoA synthase, at 657,164 to 658,069
_RS02960
RR42_RS02965: RR42_RS02965 - malonate decarboxylase subunit alpha, at 658,069 to 659,727
_RS02965
Position (kb) 657
658
659 Strain fitness (log2 ratio) -1
0
1
2 at 656.186 kb on + strand, within RR42_RS02950 at 656.186 kb on + strand, within RR42_RS02950 at 656.264 kb on + strand, within RR42_RS02950 at 657.165 kb on + strand at 657.568 kb on - strand, within RR42_RS02960 at 657.568 kb on - strand, within RR42_RS02960 at 657.670 kb on + strand, within RR42_RS02960 at 657.671 kb on - strand, within RR42_RS02960 at 658.053 kb on - strand at 658.100 kb on + strand at 658.319 kb on + strand, within RR42_RS02965 at 658.358 kb on - strand, within RR42_RS02965 at 658.370 kb on + strand, within RR42_RS02965 at 658.371 kb on - strand, within RR42_RS02965 at 658.371 kb on - strand, within RR42_RS02965 at 658.769 kb on + strand, within RR42_RS02965 at 658.769 kb on + strand, within RR42_RS02965 at 658.769 kb on + strand, within RR42_RS02965 at 658.770 kb on - strand, within RR42_RS02965 at 658.770 kb on - strand, within RR42_RS02965 at 658.869 kb on - strand, within RR42_RS02965 at 658.941 kb on + strand, within RR42_RS02965 at 658.985 kb on + strand, within RR42_RS02965
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 656,186 + RR42_RS02950 0.22 +2.0 656,186 + RR42_RS02950 0.22 -0.9 656,264 + RR42_RS02950 0.31 +0.5 657,165 + +0.4 657,568 - RR42_RS02960 0.45 +0.1 657,568 - RR42_RS02960 0.45 +0.6 657,670 + RR42_RS02960 0.56 +1.9 657,671 - RR42_RS02960 0.56 +0.8 658,053 - -0.1 658,100 + -0.1 658,319 + RR42_RS02965 0.15 +0.3 658,358 - RR42_RS02965 0.17 +0.0 658,370 + RR42_RS02965 0.18 -0.3 658,371 - RR42_RS02965 0.18 +0.6 658,371 - RR42_RS02965 0.18 -0.9 658,769 + RR42_RS02965 0.42 -1.2 658,769 + RR42_RS02965 0.42 +0.4 658,769 + RR42_RS02965 0.42 +0.4 658,770 - RR42_RS02965 0.42 -0.6 658,770 - RR42_RS02965 0.42 +0.1 658,869 - RR42_RS02965 0.48 -0.5 658,941 + RR42_RS02965 0.53 -0.8 658,985 + RR42_RS02965 0.55 +1.6
Or see this region's nucleotide sequence