Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01825 and RR42_RS01830 are separated by 140 nucleotides RR42_RS01830 and RR42_RS01835 are separated by 118 nucleotides RR42_RS01835 and RR42_RS01840 are separated by 164 nucleotides
RR42_RS01825: RR42_RS01825 - phosphoglyceromutase, at 414,874 to 415,620
_RS01825
RR42_RS01830: RR42_RS01830 - sulfurtransferase, at 415,761 to 416,174
_RS01830
RR42_RS01835: RR42_RS01835 - glutaredoxin, at 416,293 to 416,550
_RS01835
RR42_RS01840: RR42_RS01840 - preprotein translocase subunit SecB, at 416,715 to 417,233
_RS01840
Position (kb) 415
416
417 Strain fitness (log2 ratio) -2
-1
0
1 at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 415.664 kb on - strand at 415.685 kb on - strand at 415.725 kb on + strand at 415.725 kb on + strand at 415.726 kb on - strand at 415.726 kb on - strand at 415.727 kb on + strand at 415.727 kb on + strand at 415.728 kb on - strand at 415.780 kb on - strand at 415.780 kb on - strand at 415.944 kb on + strand, within RR42_RS01830 at 415.944 kb on + strand, within RR42_RS01830 at 415.945 kb on - strand, within RR42_RS01830 at 416.103 kb on + strand, within RR42_RS01830 at 416.104 kb on - strand, within RR42_RS01830 at 416.116 kb on - strand, within RR42_RS01830 at 416.116 kb on - strand, within RR42_RS01830 at 416.172 kb on + strand at 416.173 kb on - strand at 416.173 kb on - strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.307 kb on - strand at 416.311 kb on + strand at 416.312 kb on - strand at 416.321 kb on + strand, within RR42_RS01835 at 416.321 kb on + strand, within RR42_RS01835 at 416.322 kb on - strand, within RR42_RS01835 at 416.322 kb on - strand, within RR42_RS01835 at 416.464 kb on + strand, within RR42_RS01835 at 416.464 kb on + strand, within RR42_RS01835 at 416.596 kb on + strand at 416.596 kb on + strand at 416.596 kb on + strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.603 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 414,793 - +0.5 414,793 - -0.8 414,793 - +1.1 414,793 - -0.1 414,793 - -0.4 414,794 + -0.7 414,794 + -0.0 414,794 + -0.4 414,794 + -0.6 414,794 + +0.3 414,794 + -0.5 414,794 + +0.7 414,794 + +1.4 414,795 - -0.1 414,795 - -0.3 414,795 - +0.2 414,795 - -0.6 414,795 - -0.0 414,795 - +0.4 414,795 - +0.2 414,795 - -0.4 415,664 - -0.9 415,685 - +0.8 415,725 + -0.1 415,725 + +0.1 415,726 - +0.1 415,726 - -0.0 415,727 + -0.4 415,727 + -0.3 415,728 - +0.1 415,780 - -0.6 415,780 - -0.2 415,944 + RR42_RS01830 0.44 -2.7 415,944 + RR42_RS01830 0.44 -0.8 415,945 - RR42_RS01830 0.44 +0.0 416,103 + RR42_RS01830 0.83 -1.3 416,104 - RR42_RS01830 0.83 -0.5 416,116 - RR42_RS01830 0.86 -1.2 416,116 - RR42_RS01830 0.86 -0.6 416,172 + +0.1 416,173 - -0.1 416,173 - -0.1 416,306 + +0.6 416,306 + -0.7 416,306 + +0.4 416,306 + -1.3 416,306 + -0.3 416,306 + -0.5 416,307 - -1.0 416,311 + +0.5 416,312 - -0.6 416,321 + RR42_RS01835 0.11 +0.5 416,321 + RR42_RS01835 0.11 -0.7 416,322 - RR42_RS01835 0.11 +0.4 416,322 - RR42_RS01835 0.11 -0.4 416,464 + RR42_RS01835 0.66 -0.5 416,464 + RR42_RS01835 0.66 -0.6 416,596 + +0.1 416,596 + -0.5 416,596 + -0.2 416,597 - +0.8 416,597 - -0.5 416,597 - +1.2 416,597 - -0.4 416,601 + -0.3 416,601 + -2.0 416,601 + +0.3 416,601 + +0.1 416,601 + -2.1 416,601 + -0.0 416,601 + -0.1 416,603 + +0.4
Or see this region's nucleotide sequence