Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01530 and RR42_RS01535 are separated by 4 nucleotides RR42_RS01535 and RR42_RS01540 are separated by 13 nucleotides
RR42_RS01530: RR42_RS01530 - isopropylmalate isomerase, at 344,481 to 345,899
_RS01530
RR42_RS01535: RR42_RS01535 - 3-isopropylmalate dehydratase, at 345,904 to 346,551
_RS01535
RR42_RS01540: RR42_RS01540 - alpha/beta hydrolase, at 346,565 to 347,455
_RS01540
Position (kb) 345
346
347 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 344.964 kb on + strand, within RR42_RS01530 at 345.045 kb on - strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.211 kb on - strand, within RR42_RS01530 at 345.459 kb on + strand, within RR42_RS01530 at 345.459 kb on + strand, within RR42_RS01530 at 345.461 kb on + strand, within RR42_RS01530 at 345.462 kb on - strand, within RR42_RS01530 at 345.736 kb on + strand, within RR42_RS01530 at 345.736 kb on + strand, within RR42_RS01530 at 345.737 kb on - strand, within RR42_RS01530 at 345.737 kb on - strand, within RR42_RS01530 at 345.771 kb on - strand at 345.988 kb on - strand, within RR42_RS01535 at 345.988 kb on - strand, within RR42_RS01535 at 346.044 kb on + strand, within RR42_RS01535 at 346.187 kb on - strand, within RR42_RS01535 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.794 kb on - strand, within RR42_RS01540 at 346.948 kb on + strand, within RR42_RS01540 at 346.970 kb on + strand, within RR42_RS01540 at 346.970 kb on + strand, within RR42_RS01540 at 346.970 kb on + strand, within RR42_RS01540 at 347.004 kb on - strand, within RR42_RS01540 at 347.006 kb on + strand, within RR42_RS01540 at 347.006 kb on + strand, within RR42_RS01540 at 347.007 kb on - strand, within RR42_RS01540 at 347.007 kb on - strand, within RR42_RS01540 at 347.007 kb on - strand, within RR42_RS01540 at 347.075 kb on + strand, within RR42_RS01540 at 347.076 kb on - strand, within RR42_RS01540 at 347.145 kb on - strand, within RR42_RS01540 at 347.381 kb on + strand at 347.382 kb on - strand at 347.453 kb on + strand at 347.471 kb on + strand at 347.471 kb on + strand at 347.472 kb on - strand at 347.478 kb on - strand at 347.484 kb on - strand at 347.495 kb on + strand at 347.542 kb on + strand at 347.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 344,964 + RR42_RS01530 0.34 +0.3 345,045 - RR42_RS01530 0.40 +0.6 345,210 + RR42_RS01530 0.51 +1.4 345,210 + RR42_RS01530 0.51 +0.0 345,211 - RR42_RS01530 0.51 +0.5 345,459 + RR42_RS01530 0.69 -2.9 345,459 + RR42_RS01530 0.69 +0.5 345,461 + RR42_RS01530 0.69 +1.5 345,462 - RR42_RS01530 0.69 +0.2 345,736 + RR42_RS01530 0.88 +0.7 345,736 + RR42_RS01530 0.88 +0.2 345,737 - RR42_RS01530 0.89 -0.3 345,737 - RR42_RS01530 0.89 -0.2 345,771 - -1.9 345,988 - RR42_RS01535 0.13 -0.1 345,988 - RR42_RS01535 0.13 +0.4 346,044 + RR42_RS01535 0.22 +1.4 346,187 - RR42_RS01535 0.44 +1.5 346,691 - RR42_RS01540 0.14 +0.1 346,691 - RR42_RS01540 0.14 +0.4 346,691 - RR42_RS01540 0.14 +0.6 346,793 + RR42_RS01540 0.26 -1.1 346,793 + RR42_RS01540 0.26 -0.3 346,793 + RR42_RS01540 0.26 +0.4 346,794 - RR42_RS01540 0.26 +0.3 346,948 + RR42_RS01540 0.43 +0.4 346,970 + RR42_RS01540 0.45 +0.2 346,970 + RR42_RS01540 0.45 -0.8 346,970 + RR42_RS01540 0.45 -0.4 347,004 - RR42_RS01540 0.49 +0.8 347,006 + RR42_RS01540 0.49 -0.3 347,006 + RR42_RS01540 0.49 -0.1 347,007 - RR42_RS01540 0.50 +0.9 347,007 - RR42_RS01540 0.50 -0.9 347,007 - RR42_RS01540 0.50 +0.1 347,075 + RR42_RS01540 0.57 +0.4 347,076 - RR42_RS01540 0.57 +0.3 347,145 - RR42_RS01540 0.65 +0.7 347,381 + +1.6 347,382 - +1.1 347,453 + +0.8 347,471 + -0.2 347,471 + +1.0 347,472 - +0.6 347,478 - +1.0 347,484 - -0.0 347,495 + -0.1 347,542 + +0.9 347,543 - +0.9
Or see this region's nucleotide sequence