Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS01145

Experiment: R2A with Bacitracin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS01130 and RR42_RS01135 overlap by 4 nucleotidesRR42_RS01135 and RR42_RS01140 are separated by 50 nucleotidesRR42_RS01140 and RR42_RS01145 are separated by 16 nucleotidesRR42_RS01145 and RR42_RS01150 are separated by 128 nucleotides RR42_RS01130: RR42_RS01130 - haloacid dehalogenase, at 260,340 to 261,320 _RS01130 RR42_RS01135: RR42_RS01135 - dihydroorotate oxidase, at 261,317 to 261,976 _RS01135 RR42_RS01140: RR42_RS01140 - hypothetical protein, at 262,027 to 262,260 _RS01140 RR42_RS01145: RR42_RS01145 - membrane protein, at 262,277 to 263,710 _RS01145 RR42_RS01150: RR42_RS01150 - histidine kinase, at 263,839 to 265,281 _RS01150 Position (kb) 262 263 264Strain fitness (log2 ratio) -2 -1 0 1 2at 261.974 kb on + strandat 261.975 kb on - strandat 262.028 kb on + strandat 262.250 kb on + strandat 262.250 kb on + strandat 262.375 kb on - strandat 262.476 kb on + strand, within RR42_RS01145at 262.488 kb on + strand, within RR42_RS01145at 262.488 kb on + strand, within RR42_RS01145at 262.489 kb on - strand, within RR42_RS01145at 262.554 kb on + strand, within RR42_RS01145at 262.554 kb on + strand, within RR42_RS01145at 262.555 kb on - strand, within RR42_RS01145at 262.555 kb on - strand, within RR42_RS01145at 262.555 kb on - strand, within RR42_RS01145at 262.555 kb on - strand, within RR42_RS01145at 262.596 kb on + strand, within RR42_RS01145at 262.597 kb on - strand, within RR42_RS01145at 262.615 kb on + strand, within RR42_RS01145at 262.615 kb on + strand, within RR42_RS01145at 262.616 kb on - strand, within RR42_RS01145at 262.678 kb on - strand, within RR42_RS01145at 262.678 kb on - strand, within RR42_RS01145at 262.761 kb on + strand, within RR42_RS01145at 262.788 kb on + strand, within RR42_RS01145at 262.788 kb on + strand, within RR42_RS01145at 262.788 kb on + strand, within RR42_RS01145at 262.789 kb on - strand, within RR42_RS01145at 262.878 kb on + strand, within RR42_RS01145at 262.878 kb on + strand, within RR42_RS01145at 262.879 kb on - strand, within RR42_RS01145at 262.879 kb on - strand, within RR42_RS01145at 263.259 kb on + strand, within RR42_RS01145at 263.259 kb on + strand, within RR42_RS01145at 263.259 kb on + strand, within RR42_RS01145at 263.260 kb on - strand, within RR42_RS01145at 263.260 kb on - strand, within RR42_RS01145at 263.260 kb on - strand, within RR42_RS01145at 263.260 kb on - strand, within RR42_RS01145at 263.260 kb on - strand, within RR42_RS01145at 263.260 kb on - strand, within RR42_RS01145at 263.270 kb on + strand, within RR42_RS01145at 263.270 kb on + strand, within RR42_RS01145at 263.359 kb on - strand, within RR42_RS01145at 263.524 kb on - strand, within RR42_RS01145at 263.569 kb on - strandat 263.569 kb on - strandat 263.743 kb on - strandat 263.747 kb on + strandat 263.748 kb on - strandat 263.990 kb on + strand, within RR42_RS01150at 264.021 kb on - strand, within RR42_RS01150at 264.109 kb on - strand, within RR42_RS01150at 264.109 kb on - strand, within RR42_RS01150at 264.504 kb on + strand, within RR42_RS01150at 264.505 kb on - strand, within RR42_RS01150at 264.506 kb on + strand, within RR42_RS01150at 264.506 kb on + strand, within RR42_RS01150at 264.506 kb on + strand, within RR42_RS01150at 264.506 kb on + strand, within RR42_RS01150at 264.507 kb on - strand, within RR42_RS01150at 264.507 kb on - strand, within RR42_RS01150at 264.635 kb on + strand, within RR42_RS01150

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml
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261,974 + -0.6
261,975 - -1.2
262,028 + +0.1
262,250 + -0.2
262,250 + -2.3
262,375 - +0.1
262,476 + RR42_RS01145 0.14 +1.8
262,488 + RR42_RS01145 0.15 +1.1
262,488 + RR42_RS01145 0.15 -0.0
262,489 - RR42_RS01145 0.15 -2.1
262,554 + RR42_RS01145 0.19 -0.9
262,554 + RR42_RS01145 0.19 +0.3
262,555 - RR42_RS01145 0.19 +0.6
262,555 - RR42_RS01145 0.19 -1.4
262,555 - RR42_RS01145 0.19 +0.2
262,555 - RR42_RS01145 0.19 -0.2
262,596 + RR42_RS01145 0.22 -0.9
262,597 - RR42_RS01145 0.22 -0.0
262,615 + RR42_RS01145 0.24 +0.2
262,615 + RR42_RS01145 0.24 -0.8
262,616 - RR42_RS01145 0.24 +0.5
262,678 - RR42_RS01145 0.28 +0.4
262,678 - RR42_RS01145 0.28 +0.9
262,761 + RR42_RS01145 0.34 +0.6
262,788 + RR42_RS01145 0.36 -0.5
262,788 + RR42_RS01145 0.36 -0.4
262,788 + RR42_RS01145 0.36 -0.9
262,789 - RR42_RS01145 0.36 +1.0
262,878 + RR42_RS01145 0.42 -1.4
262,878 + RR42_RS01145 0.42 +0.7
262,879 - RR42_RS01145 0.42 +0.1
262,879 - RR42_RS01145 0.42 -1.4
263,259 + RR42_RS01145 0.68 +0.2
263,259 + RR42_RS01145 0.68 +0.3
263,259 + RR42_RS01145 0.68 +0.3
263,260 - RR42_RS01145 0.69 +0.9
263,260 - RR42_RS01145 0.69 -0.7
263,260 - RR42_RS01145 0.69 +0.5
263,260 - RR42_RS01145 0.69 -0.1
263,260 - RR42_RS01145 0.69 +0.4
263,260 - RR42_RS01145 0.69 +1.3
263,270 + RR42_RS01145 0.69 +0.1
263,270 + RR42_RS01145 0.69 -0.1
263,359 - RR42_RS01145 0.75 +0.4
263,524 - RR42_RS01145 0.87 +0.3
263,569 - +0.7
263,569 - +0.2
263,743 - +0.9
263,747 + +0.6
263,748 - +0.8
263,990 + RR42_RS01150 0.10 +0.5
264,021 - RR42_RS01150 0.13 +0.2
264,109 - RR42_RS01150 0.19 -0.1
264,109 - RR42_RS01150 0.19 -0.4
264,504 + RR42_RS01150 0.46 +0.9
264,505 - RR42_RS01150 0.46 -0.9
264,506 + RR42_RS01150 0.46 +0.3
264,506 + RR42_RS01150 0.46 -0.2
264,506 + RR42_RS01150 0.46 -0.0
264,506 + RR42_RS01150 0.46 -0.1
264,507 - RR42_RS01150 0.46 +0.5
264,507 - RR42_RS01150 0.46 -0.6
264,635 + RR42_RS01150 0.55 +0.2

Or see this region's nucleotide sequence