Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01130 and RR42_RS01135 overlap by 4 nucleotides RR42_RS01135 and RR42_RS01140 are separated by 50 nucleotides RR42_RS01140 and RR42_RS01145 are separated by 16 nucleotides RR42_RS01145 and RR42_RS01150 are separated by 128 nucleotides
RR42_RS01130: RR42_RS01130 - haloacid dehalogenase, at 260,340 to 261,320
_RS01130
RR42_RS01135: RR42_RS01135 - dihydroorotate oxidase, at 261,317 to 261,976
_RS01135
RR42_RS01140: RR42_RS01140 - hypothetical protein, at 262,027 to 262,260
_RS01140
RR42_RS01145: RR42_RS01145 - membrane protein, at 262,277 to 263,710
_RS01145
RR42_RS01150: RR42_RS01150 - histidine kinase, at 263,839 to 265,281
_RS01150
Position (kb)
262
263
264 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 261.974 kb on + strand at 261.975 kb on - strand at 262.028 kb on + strand at 262.250 kb on + strand at 262.250 kb on + strand at 262.375 kb on - strand at 262.476 kb on + strand, within RR42_RS01145 at 262.488 kb on + strand, within RR42_RS01145 at 262.488 kb on + strand, within RR42_RS01145 at 262.489 kb on - strand, within RR42_RS01145 at 262.554 kb on + strand, within RR42_RS01145 at 262.554 kb on + strand, within RR42_RS01145 at 262.555 kb on - strand, within RR42_RS01145 at 262.555 kb on - strand, within RR42_RS01145 at 262.555 kb on - strand, within RR42_RS01145 at 262.555 kb on - strand, within RR42_RS01145 at 262.596 kb on + strand, within RR42_RS01145 at 262.597 kb on - strand, within RR42_RS01145 at 262.615 kb on + strand, within RR42_RS01145 at 262.615 kb on + strand, within RR42_RS01145 at 262.616 kb on - strand, within RR42_RS01145 at 262.678 kb on - strand, within RR42_RS01145 at 262.678 kb on - strand, within RR42_RS01145 at 262.761 kb on + strand, within RR42_RS01145 at 262.788 kb on + strand, within RR42_RS01145 at 262.788 kb on + strand, within RR42_RS01145 at 262.788 kb on + strand, within RR42_RS01145 at 262.789 kb on - strand, within RR42_RS01145 at 262.878 kb on + strand, within RR42_RS01145 at 262.878 kb on + strand, within RR42_RS01145 at 262.879 kb on - strand, within RR42_RS01145 at 262.879 kb on - strand, within RR42_RS01145 at 263.259 kb on + strand, within RR42_RS01145 at 263.259 kb on + strand, within RR42_RS01145 at 263.259 kb on + strand, within RR42_RS01145 at 263.260 kb on - strand, within RR42_RS01145 at 263.260 kb on - strand, within RR42_RS01145 at 263.260 kb on - strand, within RR42_RS01145 at 263.260 kb on - strand, within RR42_RS01145 at 263.260 kb on - strand, within RR42_RS01145 at 263.260 kb on - strand, within RR42_RS01145 at 263.270 kb on + strand, within RR42_RS01145 at 263.270 kb on + strand, within RR42_RS01145 at 263.359 kb on - strand, within RR42_RS01145 at 263.524 kb on - strand, within RR42_RS01145 at 263.569 kb on - strand at 263.569 kb on - strand at 263.743 kb on - strand at 263.747 kb on + strand at 263.748 kb on - strand at 263.990 kb on + strand, within RR42_RS01150 at 264.021 kb on - strand, within RR42_RS01150 at 264.109 kb on - strand, within RR42_RS01150 at 264.109 kb on - strand, within RR42_RS01150 at 264.504 kb on + strand, within RR42_RS01150 at 264.505 kb on - strand, within RR42_RS01150 at 264.506 kb on + strand, within RR42_RS01150 at 264.506 kb on + strand, within RR42_RS01150 at 264.506 kb on + strand, within RR42_RS01150 at 264.506 kb on + strand, within RR42_RS01150 at 264.507 kb on - strand, within RR42_RS01150 at 264.507 kb on - strand, within RR42_RS01150 at 264.635 kb on + strand, within RR42_RS01150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 261,974 + -0.6 261,975 - -1.2 262,028 + +0.1 262,250 + -0.2 262,250 + -2.3 262,375 - +0.1 262,476 + RR42_RS01145 0.14 +1.8 262,488 + RR42_RS01145 0.15 +1.1 262,488 + RR42_RS01145 0.15 -0.0 262,489 - RR42_RS01145 0.15 -2.1 262,554 + RR42_RS01145 0.19 -0.9 262,554 + RR42_RS01145 0.19 +0.3 262,555 - RR42_RS01145 0.19 +0.6 262,555 - RR42_RS01145 0.19 -1.4 262,555 - RR42_RS01145 0.19 +0.2 262,555 - RR42_RS01145 0.19 -0.2 262,596 + RR42_RS01145 0.22 -0.9 262,597 - RR42_RS01145 0.22 -0.0 262,615 + RR42_RS01145 0.24 +0.2 262,615 + RR42_RS01145 0.24 -0.8 262,616 - RR42_RS01145 0.24 +0.5 262,678 - RR42_RS01145 0.28 +0.4 262,678 - RR42_RS01145 0.28 +0.9 262,761 + RR42_RS01145 0.34 +0.6 262,788 + RR42_RS01145 0.36 -0.5 262,788 + RR42_RS01145 0.36 -0.4 262,788 + RR42_RS01145 0.36 -0.9 262,789 - RR42_RS01145 0.36 +1.0 262,878 + RR42_RS01145 0.42 -1.4 262,878 + RR42_RS01145 0.42 +0.7 262,879 - RR42_RS01145 0.42 +0.1 262,879 - RR42_RS01145 0.42 -1.4 263,259 + RR42_RS01145 0.68 +0.2 263,259 + RR42_RS01145 0.68 +0.3 263,259 + RR42_RS01145 0.68 +0.3 263,260 - RR42_RS01145 0.69 +0.9 263,260 - RR42_RS01145 0.69 -0.7 263,260 - RR42_RS01145 0.69 +0.5 263,260 - RR42_RS01145 0.69 -0.1 263,260 - RR42_RS01145 0.69 +0.4 263,260 - RR42_RS01145 0.69 +1.3 263,270 + RR42_RS01145 0.69 +0.1 263,270 + RR42_RS01145 0.69 -0.1 263,359 - RR42_RS01145 0.75 +0.4 263,524 - RR42_RS01145 0.87 +0.3 263,569 - +0.7 263,569 - +0.2 263,743 - +0.9 263,747 + +0.6 263,748 - +0.8 263,990 + RR42_RS01150 0.10 +0.5 264,021 - RR42_RS01150 0.13 +0.2 264,109 - RR42_RS01150 0.19 -0.1 264,109 - RR42_RS01150 0.19 -0.4 264,504 + RR42_RS01150 0.46 +0.9 264,505 - RR42_RS01150 0.46 -0.9 264,506 + RR42_RS01150 0.46 +0.3 264,506 + RR42_RS01150 0.46 -0.2 264,506 + RR42_RS01150 0.46 -0.0 264,506 + RR42_RS01150 0.46 -0.1 264,507 - RR42_RS01150 0.46 +0.5 264,507 - RR42_RS01150 0.46 -0.6 264,635 + RR42_RS01150 0.55 +0.2
Or see this region's nucleotide sequence