Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS00835

Experiment: R2A with Bacitracin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS00825 and RR42_RS00830 are separated by 128 nucleotidesRR42_RS00830 and RR42_RS00835 are separated by 91 nucleotidesRR42_RS00835 and RR42_RS00840 are separated by 168 nucleotidesRR42_RS00840 and RR42_RS00845 overlap by 4 nucleotides RR42_RS00825: RR42_RS00825 - arginyl-tRNA synthetase, at 201,494 to 203,287 _RS00825 RR42_RS00830: RR42_RS00830 - cell division protein FtsN, at 203,416 to 204,192 _RS00830 RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922 _RS00835 RR42_RS00840: RR42_RS00840 - short-chain dehydrogenase, at 205,091 to 205,903 _RS00840 RR42_RS00845: RR42_RS00845 - metal ABC transporter substrate-binding protein, at 205,900 to 206,736 _RS00845 Position (kb) 204 205Strain fitness (log2 ratio) -3 -2 -1 0 1at 203.285 kb on + strandat 203.285 kb on + strandat 203.285 kb on + strandat 203.285 kb on + strandat 203.286 kb on - strandat 203.286 kb on - strandat 203.286 kb on - strandat 203.286 kb on - strandat 203.335 kb on + strandat 203.335 kb on + strandat 203.341 kb on + strandat 203.386 kb on + strandat 203.387 kb on - strandat 203.388 kb on + strandat 203.476 kb on - strandat 203.539 kb on + strand, within RR42_RS00830at 203.539 kb on + strand, within RR42_RS00830at 203.539 kb on + strand, within RR42_RS00830at 203.539 kb on + strand, within RR42_RS00830at 203.540 kb on - strand, within RR42_RS00830at 203.542 kb on - strand, within RR42_RS00830at 203.542 kb on - strand, within RR42_RS00830at 203.542 kb on - strand, within RR42_RS00830at 203.957 kb on + strand, within RR42_RS00830at 203.957 kb on + strand, within RR42_RS00830at 203.957 kb on + strand, within RR42_RS00830at 203.958 kb on - strand, within RR42_RS00830at 203.958 kb on - strand, within RR42_RS00830at 203.963 kb on - strand, within RR42_RS00830at 204.082 kb on + strand, within RR42_RS00830at 204.083 kb on - strand, within RR42_RS00830at 204.180 kb on + strandat 204.181 kb on - strandat 204.191 kb on - strandat 204.191 kb on - strandat 204.200 kb on + strandat 204.250 kb on + strandat 204.250 kb on + strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.251 kb on - strandat 204.384 kb on - strand, within RR42_RS00835at 204.450 kb on - strand, within RR42_RS00835at 204.731 kb on + strand, within RR42_RS00835at 204.731 kb on + strand, within RR42_RS00835at 204.731 kb on + strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.732 kb on - strand, within RR42_RS00835at 204.788 kb on + strand, within RR42_RS00835at 204.789 kb on - strand, within RR42_RS00835at 204.789 kb on - strand, within RR42_RS00835at 204.789 kb on - strand, within RR42_RS00835at 204.830 kb on + strand, within RR42_RS00835at 204.830 kb on + strand, within RR42_RS00835at 204.831 kb on - strand, within RR42_RS00835at 204.831 kb on - strand, within RR42_RS00835at 204.831 kb on - strand, within RR42_RS00835at 204.831 kb on - strand, within RR42_RS00835at 204.831 kb on - strand, within RR42_RS00835at 204.831 kb on - strand, within RR42_RS00835at 204.921 kb on - strandat 204.921 kb on - strandat 205.157 kb on + strandat 205.158 kb on - strandat 205.562 kb on + strand, within RR42_RS00840at 205.563 kb on - strand, within RR42_RS00840at 205.832 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml
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203,285 + +0.3
203,285 + -0.1
203,285 + -0.2
203,285 + +0.5
203,286 - -1.4
203,286 - -0.7
203,286 - -0.5
203,286 - +0.2
203,335 + +0.4
203,335 + +0.3
203,341 + +1.5
203,386 + +0.2
203,387 - -0.7
203,388 + -1.4
203,476 - -1.2
203,539 + RR42_RS00830 0.16 -0.9
203,539 + RR42_RS00830 0.16 -0.2
203,539 + RR42_RS00830 0.16 -0.2
203,539 + RR42_RS00830 0.16 -2.9
203,540 - RR42_RS00830 0.16 -1.4
203,542 - RR42_RS00830 0.16 -2.3
203,542 - RR42_RS00830 0.16 -2.6
203,542 - RR42_RS00830 0.16 -2.4
203,957 + RR42_RS00830 0.70 +0.2
203,957 + RR42_RS00830 0.70 -1.3
203,957 + RR42_RS00830 0.70 -2.0
203,958 - RR42_RS00830 0.70 -0.9
203,958 - RR42_RS00830 0.70 -0.4
203,963 - RR42_RS00830 0.70 +0.6
204,082 + RR42_RS00830 0.86 -0.1
204,083 - RR42_RS00830 0.86 +1.1
204,180 + -0.4
204,181 - -1.7
204,191 - -0.8
204,191 - -1.4
204,200 + -0.4
204,250 + -0.8
204,250 + +1.6
204,251 - -0.3
204,251 - +1.1
204,251 - -0.2
204,251 - -0.1
204,251 - +1.2
204,251 - +0.7
204,251 - -0.1
204,251 - -1.1
204,384 - RR42_RS00835 0.16 -0.3
204,450 - RR42_RS00835 0.26 -1.0
204,731 + RR42_RS00835 0.70 +0.1
204,731 + RR42_RS00835 0.70 +0.4
204,731 + RR42_RS00835 0.70 -0.4
204,732 - RR42_RS00835 0.70 -0.2
204,732 - RR42_RS00835 0.70 -1.2
204,732 - RR42_RS00835 0.70 +1.3
204,732 - RR42_RS00835 0.70 -2.3
204,732 - RR42_RS00835 0.70 -0.1
204,732 - RR42_RS00835 0.70 -0.5
204,732 - RR42_RS00835 0.70 -0.6
204,732 - RR42_RS00835 0.70 -0.6
204,788 + RR42_RS00835 0.79 -0.2
204,789 - RR42_RS00835 0.79 -1.2
204,789 - RR42_RS00835 0.79 -2.1
204,789 - RR42_RS00835 0.79 -0.6
204,830 + RR42_RS00835 0.85 -1.1
204,830 + RR42_RS00835 0.85 +0.4
204,831 - RR42_RS00835 0.86 +0.6
204,831 - RR42_RS00835 0.86 +0.6
204,831 - RR42_RS00835 0.86 -0.0
204,831 - RR42_RS00835 0.86 +0.1
204,831 - RR42_RS00835 0.86 +1.7
204,831 - RR42_RS00835 0.86 -0.2
204,921 - +0.4
204,921 - -0.9
205,157 + -0.3
205,158 - +0.7
205,562 + RR42_RS00840 0.58 -0.0
205,563 - RR42_RS00840 0.58 +0.3
205,832 + -0.1

Or see this region's nucleotide sequence