Experiment: ALP with Nickel (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1281 and Dsui_1282 are separated by 24 nucleotides Dsui_1282 and Dsui_1283 are separated by 23 nucleotides Dsui_1283 and Dsui_1284 overlap by 14 nucleotides Dsui_1284 and Dsui_1285 overlap by 4 nucleotides Dsui_1285 and Dsui_1286 overlap by 4 nucleotides
Dsui_1281: Dsui_1281 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 1,343,331 to 1,343,867
_1281
Dsui_1282: Dsui_1282 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 1,343,892 to 1,344,338
_1282
Dsui_1283: Dsui_1283 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 1,344,362 to 1,344,838
_1283
Dsui_1284: Dsui_1284 - hypothetical protein, at 1,344,825 to 1,345,418
_1284
Dsui_1285: Dsui_1285 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 1,345,415 to 1,346,239
_1285
Dsui_1286: Dsui_1286 - hypothetical protein, at 1,346,236 to 1,346,712
_1286
Position (kb)
1344
1345
1346 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1343.852 kb on + strand at 1343.852 kb on + strand at 1343.910 kb on + strand at 1343.911 kb on + strand at 1343.911 kb on + strand at 1343.919 kb on - strand at 1343.919 kb on - strand at 1343.919 kb on - strand at 1343.921 kb on + strand at 1343.921 kb on + strand at 1343.928 kb on - strand at 1343.929 kb on - strand at 1343.983 kb on - strand, within Dsui_1282 at 1344.088 kb on - strand, within Dsui_1282 at 1344.088 kb on - strand, within Dsui_1282 at 1344.127 kb on - strand, within Dsui_1282 at 1344.152 kb on - strand, within Dsui_1282 at 1344.199 kb on - strand, within Dsui_1282 at 1344.201 kb on + strand, within Dsui_1282 at 1344.258 kb on - strand, within Dsui_1282 at 1344.283 kb on - strand, within Dsui_1282 at 1344.285 kb on - strand, within Dsui_1282 at 1344.285 kb on - strand, within Dsui_1282 at 1344.402 kb on + strand at 1344.435 kb on - strand, within Dsui_1283 at 1344.696 kb on + strand, within Dsui_1283 at 1344.696 kb on + strand, within Dsui_1283 at 1344.731 kb on + strand, within Dsui_1283 at 1344.739 kb on - strand, within Dsui_1283 at 1344.739 kb on - strand, within Dsui_1283 at 1344.775 kb on - strand, within Dsui_1283 at 1344.779 kb on + strand, within Dsui_1283 at 1344.792 kb on - strand at 1344.820 kb on - strand at 1344.856 kb on + strand at 1344.883 kb on + strand at 1344.883 kb on + strand at 1344.883 kb on + strand at 1344.883 kb on + strand at 1344.883 kb on + strand at 1345.002 kb on + strand, within Dsui_1284 at 1345.003 kb on + strand, within Dsui_1284 at 1345.010 kb on - strand, within Dsui_1284 at 1345.011 kb on - strand, within Dsui_1284 at 1345.054 kb on + strand, within Dsui_1284 at 1345.065 kb on + strand, within Dsui_1284 at 1345.065 kb on + strand, within Dsui_1284 at 1345.101 kb on + strand, within Dsui_1284 at 1345.106 kb on + strand, within Dsui_1284 at 1345.106 kb on + strand, within Dsui_1284 at 1345.106 kb on + strand, within Dsui_1284 at 1345.109 kb on - strand, within Dsui_1284 at 1345.109 kb on - strand, within Dsui_1284 at 1345.111 kb on + strand, within Dsui_1284 at 1345.111 kb on + strand, within Dsui_1284 at 1345.111 kb on + strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.119 kb on - strand, within Dsui_1284 at 1345.121 kb on + strand, within Dsui_1284 at 1345.121 kb on + strand, within Dsui_1284 at 1345.121 kb on + strand, within Dsui_1284 at 1345.129 kb on - strand, within Dsui_1284 at 1345.129 kb on - strand, within Dsui_1284 at 1345.129 kb on - strand, within Dsui_1284 at 1345.129 kb on - strand, within Dsui_1284 at 1345.129 kb on - strand, within Dsui_1284 at 1345.129 kb on - strand, within Dsui_1284 at 1345.144 kb on - strand, within Dsui_1284 at 1345.326 kb on + strand, within Dsui_1284 at 1345.330 kb on - strand, within Dsui_1284 at 1345.339 kb on - strand, within Dsui_1284 at 1345.451 kb on - strand at 1345.451 kb on - strand at 1345.451 kb on - strand at 1345.504 kb on + strand, within Dsui_1285 at 1345.558 kb on - strand, within Dsui_1285 at 1345.564 kb on + strand, within Dsui_1285 at 1345.596 kb on - strand, within Dsui_1285 at 1345.603 kb on + strand, within Dsui_1285 at 1345.621 kb on + strand, within Dsui_1285 at 1345.621 kb on + strand, within Dsui_1285 at 1345.764 kb on + strand, within Dsui_1285 at 1345.766 kb on - strand, within Dsui_1285 at 1345.808 kb on - strand, within Dsui_1285 at 1345.897 kb on - strand, within Dsui_1285 at 1345.917 kb on - strand, within Dsui_1285 at 1345.953 kb on + strand, within Dsui_1285 at 1346.040 kb on - strand, within Dsui_1285 at 1346.047 kb on + strand, within Dsui_1285 at 1346.050 kb on - strand, within Dsui_1285 at 1346.075 kb on - strand, within Dsui_1285 at 1346.137 kb on + strand, within Dsui_1285 at 1346.327 kb on + strand, within Dsui_1286 at 1346.358 kb on - strand, within Dsui_1286
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Nickel (II) chloride 2.5 mM remove 1,343,852 + -0.9 1,343,852 + -1.2 1,343,910 + +0.3 1,343,911 + +0.3 1,343,911 + +0.2 1,343,919 - +0.9 1,343,919 - -0.7 1,343,919 - -1.1 1,343,921 + +0.4 1,343,921 + +0.1 1,343,928 - +0.9 1,343,929 - -0.1 1,343,983 - Dsui_1282 0.20 -0.7 1,344,088 - Dsui_1282 0.44 +0.9 1,344,088 - Dsui_1282 0.44 -0.1 1,344,127 - Dsui_1282 0.53 -0.7 1,344,152 - Dsui_1282 0.58 -0.7 1,344,199 - Dsui_1282 0.69 -0.7 1,344,201 + Dsui_1282 0.69 -4.0 1,344,258 - Dsui_1282 0.82 +0.9 1,344,283 - Dsui_1282 0.87 -1.7 1,344,285 - Dsui_1282 0.88 -0.1 1,344,285 - Dsui_1282 0.88 -0.1 1,344,402 + -0.1 1,344,435 - Dsui_1283 0.15 +0.9 1,344,696 + Dsui_1283 0.70 +0.3 1,344,696 + Dsui_1283 0.70 +0.1 1,344,731 + Dsui_1283 0.77 -1.0 1,344,739 - Dsui_1283 0.79 +0.9 1,344,739 - Dsui_1283 0.79 -3.3 1,344,775 - Dsui_1283 0.87 +1.3 1,344,779 + Dsui_1283 0.87 -0.5 1,344,792 - +0.9 1,344,820 - -0.7 1,344,856 + +1.2 1,344,883 + +0.2 1,344,883 + +0.2 1,344,883 + +0.2 1,344,883 + -0.1 1,344,883 + +0.7 1,345,002 + Dsui_1284 0.30 -0.3 1,345,003 + Dsui_1284 0.30 -1.0 1,345,010 - Dsui_1284 0.31 -1.7 1,345,011 - Dsui_1284 0.31 -0.7 1,345,054 + Dsui_1284 0.39 -0.6 1,345,065 + Dsui_1284 0.40 +0.1 1,345,065 + Dsui_1284 0.40 -0.3 1,345,101 + Dsui_1284 0.46 -0.4 1,345,106 + Dsui_1284 0.47 -0.3 1,345,106 + Dsui_1284 0.47 -2.6 1,345,106 + Dsui_1284 0.47 +0.1 1,345,109 - Dsui_1284 0.48 +0.9 1,345,109 - Dsui_1284 0.48 -1.7 1,345,111 + Dsui_1284 0.48 -0.5 1,345,111 + Dsui_1284 0.48 +0.1 1,345,111 + Dsui_1284 0.48 -0.2 1,345,119 - Dsui_1284 0.49 +0.9 1,345,119 - Dsui_1284 0.49 +0.9 1,345,119 - Dsui_1284 0.49 -1.7 1,345,119 - Dsui_1284 0.49 -1.1 1,345,119 - Dsui_1284 0.49 -1.7 1,345,119 - Dsui_1284 0.49 -0.1 1,345,119 - Dsui_1284 0.49 -0.1 1,345,121 + Dsui_1284 0.50 -0.8 1,345,121 + Dsui_1284 0.50 -0.1 1,345,121 + Dsui_1284 0.50 +0.1 1,345,129 - Dsui_1284 0.51 -0.1 1,345,129 - Dsui_1284 0.51 -1.1 1,345,129 - Dsui_1284 0.51 -1.9 1,345,129 - Dsui_1284 0.51 -2.4 1,345,129 - Dsui_1284 0.51 -1.9 1,345,129 - Dsui_1284 0.51 -1.9 1,345,144 - Dsui_1284 0.54 +0.9 1,345,326 + Dsui_1284 0.84 -0.2 1,345,330 - Dsui_1284 0.85 -1.7 1,345,339 - Dsui_1284 0.87 -1.1 1,345,451 - +0.9 1,345,451 - +0.9 1,345,451 - -1.4 1,345,504 + Dsui_1285 0.11 -0.2 1,345,558 - Dsui_1285 0.17 -0.1 1,345,564 + Dsui_1285 0.18 -1.0 1,345,596 - Dsui_1285 0.22 +1.3 1,345,603 + Dsui_1285 0.23 -0.8 1,345,621 + Dsui_1285 0.25 +0.8 1,345,621 + Dsui_1285 0.25 -0.3 1,345,764 + Dsui_1285 0.42 -0.4 1,345,766 - Dsui_1285 0.43 -1.1 1,345,808 - Dsui_1285 0.48 -0.1 1,345,897 - Dsui_1285 0.58 +0.4 1,345,917 - Dsui_1285 0.61 +0.9 1,345,953 + Dsui_1285 0.65 +0.1 1,346,040 - Dsui_1285 0.76 -1.1 1,346,047 + Dsui_1285 0.77 +0.7 1,346,050 - Dsui_1285 0.77 +0.9 1,346,075 - Dsui_1285 0.80 -0.1 1,346,137 + Dsui_1285 0.88 -0.0 1,346,327 + Dsui_1286 0.19 -0.2 1,346,358 - Dsui_1286 0.26 +0.9
Or see this region's nucleotide sequence