Experiment: ALP with Nickel (II) chloride 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0947 and Dsui_0948 are separated by 5 nucleotides Dsui_0948 and Dsui_0949 are separated by 40 nucleotides Dsui_0949 and Dsui_0950 overlap by 4 nucleotides Dsui_0950 and Dsui_0951 are separated by 169 nucleotides
Dsui_0947: Dsui_0947 - hypothetical protein, at 974,567 to 975,425
_0947
Dsui_0948: Dsui_0948 - hypothetical protein, at 975,431 to 975,850
_0948
Dsui_0949: Dsui_0949 - hypothetical protein, at 975,891 to 976,106
_0949
Dsui_0950: Dsui_0950 - acyl dehydratase, at 976,103 to 976,603
_0950
Dsui_0951: Dsui_0951 - nucleoside-diphosphate-sugar epimerase, at 976,773 to 977,732
_0951
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 974.959 kb on + strand, within Dsui_0947 at 975.083 kb on - strand, within Dsui_0947 at 975.355 kb on - strand at 975.410 kb on - strand at 975.468 kb on + strand at 975.543 kb on + strand at 975.590 kb on + strand, within Dsui_0948 at 975.599 kb on - strand at 975.918 kb on - strand, within Dsui_0949 at 976.147 kb on + strand at 976.398 kb on + strand, within Dsui_0950 at 976.530 kb on - strand, within Dsui_0950 at 976.642 kb on - strand at 976.700 kb on + strand at 976.700 kb on + strand at 976.893 kb on + strand, within Dsui_0951 at 976.893 kb on + strand, within Dsui_0951 at 977.020 kb on - strand, within Dsui_0951 at 977.048 kb on + strand, within Dsui_0951
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Nickel (II) chloride 2.5 mM remove 974,959 + Dsui_0947 0.46 -0.0 975,083 - Dsui_0947 0.60 -0.6 975,355 - -0.6 975,410 - +1.0 975,468 + +1.0 975,543 + +1.0 975,590 + Dsui_0948 0.38 +1.0 975,599 - +1.0 975,918 - Dsui_0949 0.12 -1.5 976,147 + +1.0 976,398 + Dsui_0950 0.59 -3.0 976,530 - Dsui_0950 0.85 -0.2 976,642 - +1.0 976,700 + +1.0 976,700 + +1.0 976,893 + Dsui_0951 0.12 +0.9 976,893 + Dsui_0951 0.12 -0.1 977,020 - Dsui_0951 0.26 +1.0 977,048 + Dsui_0951 0.29 -0.0
Or see this region's nucleotide sequence