Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13270 and BWI76_RS13275 are separated by 38 nucleotides BWI76_RS13275 and BWI76_RS13280 are separated by 68 nucleotides BWI76_RS13280 and BWI76_RS13285 are separated by 172 nucleotides BWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides
BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612
_RS13270
BWI76_RS13275: BWI76_RS13275 - hypothetical protein, at 2,768,651 to 2,768,836
_RS13275
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621
_RS13285
BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358
_RS13290
Position (kb)
2768
2769
2770 Strain fitness (log2 ratio)
-1
0
1
2 at 2767.984 kb on - strand, within BWI76_RS13270 at 2768.034 kb on - strand, within BWI76_RS13270 at 2768.044 kb on - strand, within BWI76_RS13270 at 2768.068 kb on - strand, within BWI76_RS13270 at 2768.281 kb on + strand, within BWI76_RS13270 at 2768.573 kb on - strand at 2768.763 kb on + strand, within BWI76_RS13275 at 2769.007 kb on - strand, within BWI76_RS13280 at 2769.080 kb on - strand, within BWI76_RS13280 at 2769.505 kb on - strand, within BWI76_RS13280 at 2769.507 kb on - strand, within BWI76_RS13280 at 2769.574 kb on - strand, within BWI76_RS13280 at 2769.577 kb on - strand, within BWI76_RS13280 at 2769.700 kb on + strand, within BWI76_RS13280 at 2769.728 kb on - strand, within BWI76_RS13280 at 2769.757 kb on - strand at 2769.783 kb on - strand at 2769.794 kb on + strand at 2770.020 kb on + strand at 2770.256 kb on + strand, within BWI76_RS13285 at 2770.653 kb on + strand at 2770.661 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 2,767,984 - BWI76_RS13270 0.73 -0.1 2,768,034 - BWI76_RS13270 0.76 +0.2 2,768,044 - BWI76_RS13270 0.76 -0.1 2,768,068 - BWI76_RS13270 0.77 -0.4 2,768,281 + BWI76_RS13270 0.86 +0.4 2,768,573 - +1.3 2,768,763 + BWI76_RS13275 0.60 +0.3 2,769,007 - BWI76_RS13280 0.11 +2.4 2,769,080 - BWI76_RS13280 0.19 +1.9 2,769,505 - BWI76_RS13280 0.65 +0.7 2,769,507 - BWI76_RS13280 0.65 +1.0 2,769,574 - BWI76_RS13280 0.72 +1.0 2,769,577 - BWI76_RS13280 0.73 +1.4 2,769,700 + BWI76_RS13280 0.86 +0.8 2,769,728 - BWI76_RS13280 0.89 +1.7 2,769,757 - +1.2 2,769,783 - +1.9 2,769,794 + +1.3 2,770,020 + +1.7 2,770,256 + BWI76_RS13285 0.41 +0.0 2,770,653 + +0.0 2,770,661 - -0.6
Or see this region's nucleotide sequence