Experiment: ALP with Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0309 and Dsui_0310 are separated by 50 nucleotides Dsui_0310 and Dsui_0311 are separated by 99 nucleotides Dsui_0311 and Dsui_0312 are separated by 14 nucleotides Dsui_0312 and Dsui_0313 are separated by 45 nucleotides
Dsui_0309: Dsui_0309 - DNA/RNA helicase, superfamily II, at 311,154 to 312,485
_0309
Dsui_0310: Dsui_0310 - acyl-CoA hydrolase, at 312,536 to 313,036
_0310
Dsui_0311: Dsui_0311 - ATP-dependent DNA helicase RecG, at 313,136 to 315,211
_0311
Dsui_0312: Dsui_0312 - endoribonuclease L-PSP, putative, at 315,226 to 315,606
_0312
Dsui_0313: Dsui_0313 - putative transcriptional regulator, at 315,652 to 316,005
_0313
Position (kb) 313
314
315
316 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 312.181 kb on + strand, within Dsui_0309 at 312.394 kb on + strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.491 kb on + strand at 312.750 kb on + strand, within Dsui_0310 at 312.805 kb on + strand, within Dsui_0310 at 312.805 kb on + strand at 312.876 kb on - strand, within Dsui_0310 at 312.917 kb on + strand at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.925 kb on - strand, within Dsui_0310 at 312.947 kb on - strand, within Dsui_0310 at 313.031 kb on + strand at 313.064 kb on + strand at 313.080 kb on - strand at 313.100 kb on + strand at 313.124 kb on - strand at 313.140 kb on + strand at 313.148 kb on - strand at 313.153 kb on + strand at 313.224 kb on + strand at 313.267 kb on + strand at 313.267 kb on + strand at 313.295 kb on + strand at 313.370 kb on - strand, within Dsui_0311 at 313.412 kb on - strand, within Dsui_0311 at 313.544 kb on + strand at 313.643 kb on + strand, within Dsui_0311 at 313.773 kb on - strand, within Dsui_0311 at 314.076 kb on - strand, within Dsui_0311 at 314.106 kb on + strand at 314.110 kb on + strand, within Dsui_0311 at 314.144 kb on - strand, within Dsui_0311 at 314.190 kb on + strand at 314.346 kb on + strand at 314.643 kb on + strand, within Dsui_0311 at 314.659 kb on + strand, within Dsui_0311 at 314.687 kb on + strand at 314.756 kb on + strand at 314.941 kb on - strand, within Dsui_0311 at 315.022 kb on + strand at 315.045 kb on + strand at 315.129 kb on - strand at 315.129 kb on - strand at 315.224 kb on - strand at 315.664 kb on + strand at 315.725 kb on - strand, within Dsui_0313 at 315.727 kb on - strand at 315.741 kb on + strand, within Dsui_0313 at 315.741 kb on + strand at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.749 kb on - strand, within Dsui_0313 at 315.756 kb on - strand, within Dsui_0313 at 315.776 kb on + strand, within Dsui_0313 at 315.851 kb on - strand, within Dsui_0313 at 315.967 kb on + strand at 315.999 kb on + strand at 316.089 kb on - strand at 316.091 kb on + strand at 316.109 kb on + strand at 316.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Nickel (II) chloride 1.5 mM remove 312,181 + Dsui_0309 0.77 -2.0 312,394 + -1.0 312,488 - -1.4 312,488 - -2.1 312,491 + +0.6 312,750 + Dsui_0310 0.43 -0.4 312,805 + Dsui_0310 0.54 -1.4 312,805 + +0.6 312,876 - Dsui_0310 0.68 -1.4 312,917 + -1.0 312,925 - Dsui_0310 0.78 -0.5 312,925 - Dsui_0310 0.78 -1.3 312,925 - Dsui_0310 0.78 -1.3 312,925 - Dsui_0310 0.78 -2.1 312,947 - Dsui_0310 0.82 -1.8 313,031 + +0.6 313,064 + +0.6 313,080 - -1.5 313,100 + +0.6 313,124 - -0.4 313,140 + +0.6 313,148 - -1.2 313,153 + -1.0 313,224 + +0.6 313,267 + -2.6 313,267 + -0.4 313,295 + -1.0 313,370 - Dsui_0311 0.11 -0.3 313,412 - Dsui_0311 0.13 -1.0 313,544 + +0.6 313,643 + Dsui_0311 0.24 +0.6 313,773 - Dsui_0311 0.31 -1.9 314,076 - Dsui_0311 0.45 -1.5 314,106 + +0.6 314,110 + Dsui_0311 0.47 +0.6 314,144 - Dsui_0311 0.49 -2.1 314,190 + -1.0 314,346 + -1.4 314,643 + Dsui_0311 0.73 -0.4 314,659 + Dsui_0311 0.73 -2.2 314,687 + +0.6 314,756 + +0.6 314,941 - Dsui_0311 0.87 -1.5 315,022 + +0.6 315,045 + -0.4 315,129 - -1.9 315,129 - -2.1 315,224 - -1.3 315,664 + +0.6 315,725 - Dsui_0313 0.21 -1.4 315,727 - -0.4 315,741 + Dsui_0313 0.25 -0.4 315,741 + +1.6 315,749 - Dsui_0313 0.27 -2.0 315,749 - Dsui_0313 0.27 -3.3 315,749 - Dsui_0313 0.27 +0.6 315,749 - Dsui_0313 0.27 -1.7 315,749 - Dsui_0313 0.27 -0.4 315,756 - Dsui_0313 0.29 -1.4 315,776 + Dsui_0313 0.35 -2.7 315,851 - Dsui_0313 0.56 +0.6 315,967 + +0.6 315,999 + -1.0 316,089 - -2.4 316,091 + -1.4 316,109 + -1.0 316,168 - -1.3
Or see this region's nucleotide sequence