Strain Fitness in Shewanella oneidensis MR-1 around SO_t043

Experiment: Vanillin 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgapA-3 and tRNA-Gly-7 are separated by 296 nucleotidestRNA-Gly-7 and tRNA-Cys-2 are separated by 20 nucleotidestRNA-Cys-2 and tRNA-Leu-6 are separated by 49 nucleotidestRNA-Leu-6 and SO2349 are separated by 23 nucleotidesSO2349 and aspC-1 are separated by 158 nucleotides SO2347: gapA-3 - glyceraldehyde 3-phosphate dehydrogenase (NCBI ptt file), at 2,455,113 to 2,456,546 gapA-3 SO_t041: tRNA-Gly-7 - tRNA-Gly (RefSeq), at 2,456,843 to 2,456,918 tRNA-Gly-7 SO_t042: tRNA-Cys-2 - tRNA-Cys (RefSeq), at 2,456,939 to 2,457,012 tRNA-Cys-2 SO_t043: tRNA-Leu-6 - tRNA-Leu (RefSeq), at 2,457,062 to 2,457,148 tRNA-Leu-6 SO2349: SO2349 - hypothetical protein (NCBI ptt file), at 2,457,172 to 2,457,270 SO2349 SO2350: aspC-1 - aspartate aminotransferase (NCBI ptt file), at 2,457,429 to 2,458,622 aspC-1 Position (kb) 2457 2458Strain fitness (log2 ratio) -3 -2 -1 0 1at 2456.065 kb on - strand, within gapA-3at 2456.106 kb on - strand, within gapA-3at 2456.106 kb on - strand, within gapA-3at 2456.124 kb on + strand, within gapA-3at 2456.703 kb on + strandat 2456.781 kb on + strandat 2457.364 kb on + strandat 2457.364 kb on + strandat 2457.443 kb on + strandat 2457.451 kb on - strandat 2457.663 kb on + strand, within aspC-1at 2457.663 kb on + strand, within aspC-1at 2457.671 kb on - strand, within aspC-1at 2457.671 kb on - strand, within aspC-1at 2457.689 kb on - strand, within aspC-1at 2457.815 kb on + strand, within aspC-1at 2457.929 kb on + strand, within aspC-1at 2457.981 kb on - strand, within aspC-1at 2458.028 kb on + strand, within aspC-1at 2458.036 kb on - strand, within aspC-1at 2458.105 kb on + strand, within aspC-1at 2458.118 kb on - strand, within aspC-1

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Per-strain Table

Position Strand Gene LocusTag Fraction Vanillin 2.5 mM
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2,456,065 - gapA-3 SO2347 0.66 -2.3
2,456,106 - gapA-3 SO2347 0.69 +0.7
2,456,106 - gapA-3 SO2347 0.69 -0.8
2,456,124 + gapA-3 SO2347 0.71 -1.6
2,456,703 + -0.4
2,456,781 + +1.7
2,457,364 + -0.1
2,457,364 + -0.6
2,457,443 + -2.2
2,457,451 - -1.1
2,457,663 + aspC-1 SO2350 0.20 -0.7
2,457,663 + aspC-1 SO2350 0.20 -0.9
2,457,671 - aspC-1 SO2350 0.20 +0.3
2,457,671 - aspC-1 SO2350 0.20 -0.8
2,457,689 - aspC-1 SO2350 0.22 +0.4
2,457,815 + aspC-1 SO2350 0.32 +0.2
2,457,929 + aspC-1 SO2350 0.42 -0.8
2,457,981 - aspC-1 SO2350 0.46 -1.3
2,458,028 + aspC-1 SO2350 0.50 -3.0
2,458,036 - aspC-1 SO2350 0.51 -1.6
2,458,105 + aspC-1 SO2350 0.57 -1.7
2,458,118 - aspC-1 SO2350 0.58 -1.7

Or see this region's nucleotide sequence