Experiment: Vanillin 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadE and SO2022 are separated by 60 nucleotides SO2022 and SO2024 are separated by 409 nucleotides SO2024 and tRNA-Pro-4 are separated by 188 nucleotides tRNA-Pro-4 and SO2025 are separated by 140 nucleotides SO2025 and SO2026 are separated by 59 nucleotides
SO2021: nadE - NH(3)-dependent NAD(+) synthetase (NCBI ptt file), at 2,120,953 to 2,121,783
nadE
SO2022: SO2022 - hypothetical protein (NCBI ptt file), at 2,121,844 to 2,122,368
SO2022
SO2024: SO2024 - hypothetical protein (NCBI ptt file), at 2,122,778 to 2,124,004
SO2024
SO_t037: tRNA-Pro-4 - tRNA-Pro (RefSeq), at 2,124,193 to 2,124,269
tRNA-Pro-4
SO2025: SO2025 - ISSod1, transposase OrfA (NCBI ptt file), at 2,124,410 to 2,124,700
SO2025
SO2026: SO2026 - ISSod1, transposase OrfB (NCBI ptt file), at 2,124,760 to 2,125,569
SO2026
Position (kb)
2122
2123
2124
2125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2121.920 kb on + strand, within SO2022 at 2121.920 kb on + strand, within SO2022 at 2121.922 kb on - strand, within SO2022 at 2121.948 kb on - strand, within SO2022 at 2121.963 kb on - strand, within SO2022 at 2122.043 kb on + strand, within SO2022 at 2122.043 kb on + strand, within SO2022 at 2122.067 kb on + strand, within SO2022 at 2122.075 kb on + strand, within SO2022 at 2122.311 kb on + strand, within SO2022 at 2122.371 kb on - strand at 2122.389 kb on - strand at 2122.414 kb on - strand at 2122.420 kb on - strand at 2122.464 kb on - strand at 2122.517 kb on + strand at 2122.517 kb on + strand at 2122.517 kb on + strand at 2122.525 kb on - strand at 2122.556 kb on - strand at 2123.045 kb on + strand, within SO2024 at 2123.100 kb on - strand, within SO2024 at 2123.328 kb on + strand, within SO2024 at 2123.507 kb on + strand, within SO2024 at 2123.786 kb on + strand, within SO2024 at 2123.786 kb on + strand, within SO2024 at 2123.794 kb on - strand, within SO2024 at 2123.794 kb on - strand, within SO2024 at 2124.016 kb on - strand at 2124.087 kb on + strand at 2124.220 kb on + strand, within tRNA-Pro-4 at 2124.222 kb on + strand, within tRNA-Pro-4 at 2124.222 kb on + strand, within tRNA-Pro-4 at 2124.222 kb on + strand, within tRNA-Pro-4 at 2124.347 kb on - strand at 2124.357 kb on - strand at 2124.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 2.5 mM remove 2,121,920 + SO2022 0.14 -1.0 2,121,920 + SO2022 0.14 +0.4 2,121,922 - SO2022 0.15 -0.1 2,121,948 - SO2022 0.20 -0.0 2,121,963 - SO2022 0.23 -0.8 2,122,043 + SO2022 0.38 -0.5 2,122,043 + SO2022 0.38 -0.1 2,122,067 + SO2022 0.42 -0.6 2,122,075 + SO2022 0.44 -0.8 2,122,311 + SO2022 0.89 +1.8 2,122,371 - +0.4 2,122,389 - -0.2 2,122,414 - +0.2 2,122,420 - +0.7 2,122,464 - +1.0 2,122,517 + -0.0 2,122,517 + -0.1 2,122,517 + -0.8 2,122,525 - -0.2 2,122,556 - -0.6 2,123,045 + SO2024 0.22 -1.0 2,123,100 - SO2024 0.26 -0.6 2,123,328 + SO2024 0.45 -0.2 2,123,507 + SO2024 0.59 -0.4 2,123,786 + SO2024 0.82 -1.8 2,123,786 + SO2024 0.82 -0.5 2,123,794 - SO2024 0.83 -0.7 2,123,794 - SO2024 0.83 -0.5 2,124,016 - -0.2 2,124,087 + +0.4 2,124,220 + tRNA-Pro-4 SO_t037 0.35 +1.6 2,124,222 + tRNA-Pro-4 SO_t037 0.38 -0.2 2,124,222 + tRNA-Pro-4 SO_t037 0.38 +1.8 2,124,222 + tRNA-Pro-4 SO_t037 0.38 +0.9 2,124,347 - +0.5 2,124,357 - -0.1 2,124,358 - -0.4
Or see this region's nucleotide sequence