Experiment: Vanillin 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrC-2 and nqrD-2 overlap by 1 nucleotides nqrD-2 and nqrE-2 are separated by 5 nucleotides nqrE-2 and nqrF-2 are separated by 46 nucleotides
SO1105: nqrC-2 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 1,148,762 to 1,149,550
nqrC-2
SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182
nqrD-2
SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796
nqrE-2
SO1108: nqrF-2 - NADH:ubiquinone oxidoreductase, Na translocating, beta subunit (NCBI ptt file), at 1,150,843 to 1,152,099
nqrF-2
Position (kb) 1150
1151 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1149.336 kb on - strand, within nqrC-2 at 1149.408 kb on - strand, within nqrC-2 at 1149.774 kb on - strand, within nqrD-2 at 1150.157 kb on - strand at 1150.323 kb on + strand, within nqrE-2 at 1150.427 kb on - strand, within nqrE-2 at 1150.539 kb on + strand, within nqrE-2 at 1150.556 kb on - strand, within nqrE-2 at 1150.638 kb on + strand, within nqrE-2
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 1 mM remove 1,149,336 - nqrC-2 SO1105 0.73 -2.3 1,149,408 - nqrC-2 SO1105 0.82 -3.0 1,149,774 - nqrD-2 SO1106 0.35 -1.6 1,150,157 - -3.1 1,150,323 + nqrE-2 SO1107 0.22 -1.8 1,150,427 - nqrE-2 SO1107 0.39 -2.0 1,150,539 + nqrE-2 SO1107 0.58 -3.3 1,150,556 - nqrE-2 SO1107 0.60 -2.0 1,150,638 + nqrE-2 SO1107 0.74 -2.7
Or see this region's nucleotide sequence