Strain Fitness in Shewanella oneidensis MR-1 around SO_t037

Experiment: 5-Hydroxymethylfurfural 15 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2024 and tRNA-Pro-4 are separated by 188 nucleotidestRNA-Pro-4 and SO2025 are separated by 140 nucleotidesSO2025 and SO2026 are separated by 59 nucleotides SO2024: SO2024 - hypothetical protein (NCBI ptt file), at 2,122,778 to 2,124,004 SO2024 SO_t037: tRNA-Pro-4 - tRNA-Pro (RefSeq), at 2,124,193 to 2,124,269 tRNA-Pro-4 SO2025: SO2025 - ISSod1, transposase OrfA (NCBI ptt file), at 2,124,410 to 2,124,700 SO2025 SO2026: SO2026 - ISSod1, transposase OrfB (NCBI ptt file), at 2,124,760 to 2,125,569 SO2026 Position (kb) 2124 2125Strain fitness (log2 ratio) -1 0 1at 2123.328 kb on + strand, within SO2024at 2123.507 kb on + strand, within SO2024at 2123.786 kb on + strand, within SO2024at 2123.786 kb on + strand, within SO2024at 2123.794 kb on - strand, within SO2024at 2123.794 kb on - strand, within SO2024at 2124.016 kb on - strandat 2124.087 kb on + strandat 2124.220 kb on + strand, within tRNA-Pro-4at 2124.222 kb on + strand, within tRNA-Pro-4at 2124.222 kb on + strand, within tRNA-Pro-4at 2124.222 kb on + strand, within tRNA-Pro-4at 2124.347 kb on - strandat 2124.357 kb on - strandat 2124.358 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 15 mM
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2,123,328 + SO2024 0.45 -0.0
2,123,507 + SO2024 0.59 -0.2
2,123,786 + SO2024 0.82 +0.4
2,123,786 + SO2024 0.82 -0.2
2,123,794 - SO2024 0.83 -0.2
2,123,794 - SO2024 0.83 -1.6
2,124,016 - -0.3
2,124,087 + -0.1
2,124,220 + tRNA-Pro-4 SO_t037 0.35 +0.1
2,124,222 + tRNA-Pro-4 SO_t037 0.38 +0.4
2,124,222 + tRNA-Pro-4 SO_t037 0.38 +1.1
2,124,222 + tRNA-Pro-4 SO_t037 0.38 +0.8
2,124,347 - +0.4
2,124,357 - -0.0
2,124,358 - -0.7

Or see this region's nucleotide sequence