Experiment: 5-Hydroxymethylfurfural 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3103 and SO3104 are separated by 41 nucleotides SO3104 and pspE-1 are separated by 25 nucleotides pspE-1 and aprE are separated by 576 nucleotides
SO3103: SO3103 - AcrB/AcrD/AcrF family protein (NCBI ptt file), at 3,229,020 to 3,232,283
SO3103
SO3104: SO3104 - conserved hypothetical protein (NCBI ptt file), at 3,232,325 to 3,232,537
SO3104
SO3105: pspE-1 - phage shock protein E (NCBI ptt file), at 3,232,563 to 3,232,961
pspE-1
SO3106: aprE - cold-active serine alkaline protease (NCBI ptt file), at 3,233,538 to 3,235,961
aprE
Position (kb)
3232
3233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3231.642 kb on + strand, within SO3103 at 3231.650 kb on - strand, within SO3103 at 3231.687 kb on + strand, within SO3103 at 3231.809 kb on - strand, within SO3103 at 3231.910 kb on - strand, within SO3103 at 3232.410 kb on - strand, within SO3104 at 3232.473 kb on - strand, within SO3104 at 3232.729 kb on - strand, within pspE-1 at 3232.767 kb on + strand, within pspE-1 at 3232.922 kb on - strand at 3232.964 kb on - strand at 3233.056 kb on - strand at 3233.057 kb on + strand at 3233.581 kb on + strand at 3233.783 kb on - strand, within aprE at 3233.804 kb on - strand, within aprE at 3233.887 kb on + strand, within aprE at 3233.921 kb on - strand, within aprE
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 15 mM remove 3,231,642 + SO3103 0.80 -0.5 3,231,650 - SO3103 0.81 +0.9 3,231,687 + SO3103 0.82 +0.3 3,231,809 - SO3103 0.85 -0.2 3,231,910 - SO3103 0.89 -0.3 3,232,410 - SO3104 0.40 -0.1 3,232,473 - SO3104 0.69 +0.5 3,232,729 - pspE-1 SO3105 0.42 -1.1 3,232,767 + pspE-1 SO3105 0.51 -0.9 3,232,922 - -0.9 3,232,964 - -0.7 3,233,056 - +1.9 3,233,057 + -1.4 3,233,581 + +0.9 3,233,783 - aprE SO3106 0.10 -1.9 3,233,804 - aprE SO3106 0.11 +1.3 3,233,887 + aprE SO3106 0.14 -0.2 3,233,921 - aprE SO3106 0.16 +0.9
Or see this region's nucleotide sequence