Strain Fitness in Shewanella oneidensis MR-1 around SO1105

Experiment: 5-Hydroxymethylfurfural 15 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnqrB-2 and nqrC-2 overlap by 8 nucleotidesnqrC-2 and nqrD-2 overlap by 1 nucleotidesnqrD-2 and nqrE-2 are separated by 5 nucleotides SO1104: nqrB-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrB (NCBI ptt file), at 1,147,570 to 1,148,769 nqrB-2 SO1105: nqrC-2 - NADH:ubiquinone oxidoreductase, Na translocating, gamma subunit (NCBI ptt file), at 1,148,762 to 1,149,550 nqrC-2 SO1106: nqrD-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 1,149,550 to 1,150,182 nqrD-2 SO1107: nqrE-2 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 1,150,188 to 1,150,796 nqrE-2 Position (kb) 1148 1149 1150Strain fitness (log2 ratio) -3 -2 -1 0 1at 1147.766 kb on - strand, within nqrB-2at 1147.981 kb on + strand, within nqrB-2at 1148.277 kb on - strand, within nqrB-2at 1148.282 kb on + strand, within nqrB-2at 1148.429 kb on + strand, within nqrB-2at 1148.736 kb on + strandat 1149.336 kb on - strand, within nqrC-2at 1149.408 kb on - strand, within nqrC-2at 1149.774 kb on - strand, within nqrD-2at 1150.157 kb on - strandat 1150.323 kb on + strand, within nqrE-2at 1150.427 kb on - strand, within nqrE-2at 1150.539 kb on + strand, within nqrE-2

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 15 mM
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1,147,766 - nqrB-2 SO1104 0.16 -1.4
1,147,981 + nqrB-2 SO1104 0.34 -3.0
1,148,277 - nqrB-2 SO1104 0.59 -0.7
1,148,282 + nqrB-2 SO1104 0.59 -1.7
1,148,429 + nqrB-2 SO1104 0.72 -1.9
1,148,736 + -1.1
1,149,336 - nqrC-2 SO1105 0.73 -3.5
1,149,408 - nqrC-2 SO1105 0.82 -2.7
1,149,774 - nqrD-2 SO1106 0.35 -2.2
1,150,157 - -1.8
1,150,323 + nqrE-2 SO1107 0.22 -2.5
1,150,427 - nqrE-2 SO1107 0.39 -1.7
1,150,539 + nqrE-2 SO1107 0.58 -3.0

Or see this region's nucleotide sequence