Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2547

Experiment: methylglyoxal 0.0016 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2545 and Psest_2546 are separated by 101 nucleotidesPsest_2546 and Psest_2547 are separated by 19 nucleotidesPsest_2547 and Psest_2548 are separated by 87 nucleotides Psest_2545: Psest_2545 - recombination protein RecR, at 2,724,555 to 2,725,154 _2545 Psest_2546: Psest_2546 - DNA-binding protein, YbaB/EbfC family, at 2,725,256 to 2,725,582 _2546 Psest_2547: Psest_2547 - DNA polymerase III, subunit gamma and tau, at 2,725,602 to 2,727,614 _2547 Psest_2548: Psest_2548 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 2,727,702 to 2,728,847 _2548 Position (kb) 2725 2726 2727 2728Strain fitness (log2 ratio) -2 -1 0 1 2at 2724.694 kb on + strand, within Psest_2545at 2724.694 kb on + strand, within Psest_2545at 2724.695 kb on - strand, within Psest_2545at 2724.789 kb on - strand, within Psest_2545at 2724.848 kb on - strand, within Psest_2545at 2724.848 kb on - strand, within Psest_2545at 2725.096 kb on - strandat 2725.159 kb on - strandat 2725.250 kb on + strandat 2725.250 kb on + strandat 2725.257 kb on + strandat 2725.257 kb on + strandat 2725.258 kb on - strandat 2725.258 kb on - strandat 2725.258 kb on - strandat 2727.692 kb on + strandat 2727.692 kb on + strandat 2727.693 kb on - strandat 2727.693 kb on - strandat 2727.693 kb on - strandat 2727.693 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.0016 vol%
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2,724,694 + Psest_2545 0.23 +2.6
2,724,694 + Psest_2545 0.23 +1.5
2,724,695 - Psest_2545 0.23 -0.4
2,724,789 - Psest_2545 0.39 -0.6
2,724,848 - Psest_2545 0.49 -0.4
2,724,848 - Psest_2545 0.49 -2.1
2,725,096 - -0.9
2,725,159 - +0.3
2,725,250 + -0.3
2,725,250 + -0.9
2,725,257 + +1.5
2,725,257 + -2.6
2,725,258 - -1.0
2,725,258 - -1.9
2,725,258 - +0.7
2,727,692 + +0.9
2,727,692 + +1.0
2,727,693 - +1.3
2,727,693 - +1.1
2,727,693 - +0.5
2,727,693 - +1.0

Or see this region's nucleotide sequence