Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtgA and cysH are separated by 675 nucleotides cysH and cysI are separated by 52 nucleotides
SO3735: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI ptt file), at 3,878,719 to 3,879,495
mtgA
SO3736: cysH - phosphoadenosine phosphosulfate reductase (NCBI ptt file), at 3,880,171 to 3,880,908
cysH
SO3737: cysI - sulfite reductase (NADPH) hemoprotein beta-component (cysI) (NCBI ptt file), at 3,880,961 to 3,882,658
cysI
Position (kb)
3880
3881 Strain fitness (log2 ratio)
-2
-1
0
1 at 3879.227 kb on - strand, within mtgA at 3879.232 kb on - strand, within mtgA at 3879.254 kb on + strand, within mtgA at 3879.259 kb on + strand, within mtgA at 3879.269 kb on - strand, within mtgA at 3879.278 kb on + strand, within mtgA at 3879.278 kb on + strand, within mtgA at 3879.286 kb on - strand, within mtgA at 3879.286 kb on - strand, within mtgA at 3879.398 kb on + strand, within mtgA at 3879.405 kb on + strand, within mtgA at 3879.419 kb on + strand at 3879.435 kb on - strand at 3879.539 kb on - strand at 3879.573 kb on + strand at 3879.673 kb on + strand at 3879.673 kb on + strand at 3879.673 kb on + strand at 3879.673 kb on + strand at 3879.678 kb on + strand at 3879.683 kb on + strand at 3879.691 kb on - strand at 3879.760 kb on - strand at 3879.775 kb on + strand at 3879.869 kb on + strand at 3879.900 kb on - strand at 3879.933 kb on - strand at 3879.942 kb on - strand at 3879.950 kb on - strand at 3879.952 kb on - strand at 3880.557 kb on - strand, within cysH at 3880.582 kb on + strand, within cysH at 3880.582 kb on + strand, within cysH at 3880.610 kb on - strand, within cysH at 3880.947 kb on + strand at 3881.101 kb on - strand at 3881.774 kb on - strand, within cysI at 3881.815 kb on - strand, within cysI
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 3,879,227 - mtgA SO3735 0.65 -0.3 3,879,232 - mtgA SO3735 0.66 +0.8 3,879,254 + mtgA SO3735 0.69 +0.5 3,879,259 + mtgA SO3735 0.69 -0.3 3,879,269 - mtgA SO3735 0.71 +0.2 3,879,278 + mtgA SO3735 0.72 -0.3 3,879,278 + mtgA SO3735 0.72 -0.4 3,879,286 - mtgA SO3735 0.73 -0.4 3,879,286 - mtgA SO3735 0.73 -0.4 3,879,398 + mtgA SO3735 0.87 -0.6 3,879,405 + mtgA SO3735 0.88 +0.7 3,879,419 + +0.8 3,879,435 - -0.4 3,879,539 - -0.5 3,879,573 + -0.1 3,879,673 + -0.8 3,879,673 + -0.9 3,879,673 + -0.2 3,879,673 + +0.3 3,879,678 + +0.6 3,879,683 + +0.4 3,879,691 - +0.3 3,879,760 - +0.2 3,879,775 + +1.1 3,879,869 + -0.2 3,879,900 - +0.2 3,879,933 - -0.5 3,879,942 - -0.2 3,879,950 - +0.8 3,879,952 - +0.4 3,880,557 - cysH SO3736 0.52 -1.5 3,880,582 + cysH SO3736 0.56 -2.2 3,880,582 + cysH SO3736 0.56 -0.8 3,880,610 - cysH SO3736 0.59 +0.5 3,880,947 + -1.5 3,881,101 - -0.4 3,881,774 - cysI SO3737 0.48 -1.3 3,881,815 - cysI SO3737 0.50 -0.1
Or see this region's nucleotide sequence