Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxA and ksgA are separated by 14 nucleotides ksgA and apaG are separated by 52 nucleotides apaG and apaH are separated by 21 nucleotides
SO3638: pdxA - pyridoxal phosphate biosynthetic protein PdxA (NCBI ptt file), at 3,797,649 to 3,798,635
pdxA
SO3639: ksgA - dimethyladenosine transferase (NCBI ptt file), at 3,798,650 to 3,799,456
ksgA
SO3640: apaG - apaG protein (NCBI ptt file), at 3,799,509 to 3,799,889
apaG
SO3641: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) (NCBI ptt file), at 3,799,911 to 3,800,735
apaH
Position (kb)
3798
3799
3800 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3798.909 kb on - strand, within ksgA at 3799.193 kb on - strand, within ksgA at 3799.236 kb on + strand, within ksgA at 3799.290 kb on - strand, within ksgA at 3799.290 kb on - strand, within ksgA at 3799.458 kb on - strand at 3799.458 kb on - strand at 3799.528 kb on - strand at 3799.528 kb on - strand at 3799.695 kb on + strand, within apaG at 3799.702 kb on - strand, within apaG at 3799.710 kb on - strand, within apaG at 3799.935 kb on + strand at 3799.935 kb on + strand at 3799.943 kb on - strand at 3799.943 kb on - strand at 3799.943 kb on - strand at 3800.067 kb on - strand, within apaH at 3800.212 kb on + strand, within apaH at 3800.212 kb on + strand, within apaH at 3800.220 kb on - strand, within apaH at 3800.386 kb on + strand, within apaH at 3800.389 kb on + strand, within apaH at 3800.406 kb on - strand, within apaH at 3800.418 kb on + strand, within apaH at 3800.418 kb on + strand, within apaH at 3800.418 kb on + strand, within apaH at 3800.418 kb on + strand, within apaH at 3800.426 kb on - strand, within apaH at 3800.426 kb on - strand, within apaH at 3800.426 kb on - strand, within apaH
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 3,798,909 - ksgA SO3639 0.32 -0.8 3,799,193 - ksgA SO3639 0.67 -0.4 3,799,236 + ksgA SO3639 0.73 -1.2 3,799,290 - ksgA SO3639 0.79 +1.2 3,799,290 - ksgA SO3639 0.79 -1.0 3,799,458 - +0.1 3,799,458 - -1.6 3,799,528 - -2.9 3,799,528 - -2.4 3,799,695 + apaG SO3640 0.49 +0.2 3,799,702 - apaG SO3640 0.51 +0.1 3,799,710 - apaG SO3640 0.53 -0.1 3,799,935 + -1.2 3,799,935 + -0.2 3,799,943 - -0.9 3,799,943 - +0.4 3,799,943 - +0.3 3,800,067 - apaH SO3641 0.19 +1.1 3,800,212 + apaH SO3641 0.36 -0.6 3,800,212 + apaH SO3641 0.36 -0.2 3,800,220 - apaH SO3641 0.37 +0.1 3,800,386 + apaH SO3641 0.58 +0.4 3,800,389 + apaH SO3641 0.58 -1.3 3,800,406 - apaH SO3641 0.60 +0.4 3,800,418 + apaH SO3641 0.61 -0.5 3,800,418 + apaH SO3641 0.61 -0.2 3,800,418 + apaH SO3641 0.61 +0.2 3,800,418 + apaH SO3641 0.61 +0.8 3,800,426 - apaH SO3641 0.62 +0.1 3,800,426 - apaH SO3641 0.62 -0.7 3,800,426 - apaH SO3641 0.62 +0.2
Or see this region's nucleotide sequence