Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtrD and feoA are separated by 470 nucleotides feoA and feoB overlap by 8 nucleotides feoB and glnS are separated by 184 nucleotides
SO1782: mtrD - decaheme cytochrome c MtrD (NCBI ptt file), at 1,868,251 to 1,869,171
mtrD
SO1783: feoA - ferrous iron transport protein A (NCBI ptt file), at 1,869,642 to 1,869,881
feoA
SO1784: feoB - ferrous iron transport protein B (NCBI ptt file), at 1,869,874 to 1,872,168
feoB
SO1786: glnS - glutaminyl-tRNA synthetase (NCBI ptt file), at 1,872,353 to 1,874,023
glnS
Position (kb)
1869
1870
1871
1872
1873 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1869.063 kb on - strand, within mtrD at 1869.127 kb on + strand at 1869.133 kb on + strand at 1869.141 kb on - strand at 1869.368 kb on - strand at 1869.405 kb on + strand at 1869.405 kb on + strand at 1869.424 kb on + strand at 1869.426 kb on + strand at 1869.426 kb on + strand at 1869.510 kb on + strand at 1869.587 kb on + strand at 1869.597 kb on - strand at 1869.860 kb on + strand at 1872.125 kb on + strand at 1872.252 kb on + strand at 1872.252 kb on + strand at 1872.252 kb on + strand at 1872.260 kb on - strand at 1872.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 1,869,063 - mtrD SO1782 0.88 -0.1 1,869,127 + +2.0 1,869,133 + -1.1 1,869,141 - +1.7 1,869,368 - -0.1 1,869,405 + -0.3 1,869,405 + -3.2 1,869,424 + +0.0 1,869,426 + +2.9 1,869,426 + -0.3 1,869,510 + +0.2 1,869,587 + -1.2 1,869,597 - +0.5 1,869,860 + -0.9 1,872,125 + -4.0 1,872,252 + -0.3 1,872,252 + -0.1 1,872,252 + -1.1 1,872,260 - +0.1 1,872,260 - +0.5
Or see this region's nucleotide sequence