Experiment: 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspF and gspG are separated by 74 nucleotides gspG and gspH are separated by 1 nucleotides gspH and gspI overlap by 14 nucleotides gspI and gspJ overlap by 20 nucleotides
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
SO0169: gspG - general secretion pathway protein G (NCBI ptt file), at 177,279 to 177,713
gspG
SO0170: gspH - general secretion pathway protein H (NCBI ptt file), at 177,715 to 178,287
gspH
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
Position (kb) 177
178 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 176.289 kb on - strand, within gspF at 176.327 kb on + strand, within gspF at 176.327 kb on + strand, within gspF at 176.335 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.524 kb on - strand, within gspF at 176.555 kb on - strand, within gspF at 176.597 kb on - strand, within gspF at 176.640 kb on - strand, within gspF at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF at 177.010 kb on - strand, within gspF at 177.118 kb on + strand at 177.269 kb on + strand at 177.277 kb on - strand at 177.303 kb on + strand at 177.303 kb on + strand at 177.329 kb on + strand, within gspG at 177.364 kb on - strand, within gspG at 177.366 kb on - strand, within gspG at 177.397 kb on - strand, within gspG at 177.476 kb on + strand, within gspG at 177.492 kb on + strand, within gspG at 177.554 kb on + strand, within gspG at 177.613 kb on - strand, within gspG at 177.638 kb on + strand, within gspG at 177.662 kb on + strand, within gspG at 177.751 kb on - strand at 177.788 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.845 kb on - strand, within gspH at 177.845 kb on - strand, within gspH at 178.065 kb on - strand, within gspH at 178.221 kb on - strand, within gspH at 178.237 kb on + strand at 178.266 kb on - strand at 178.282 kb on + strand at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 10 mM remove 176,289 - gspF SO0168 0.25 -2.2 176,327 + gspF SO0168 0.28 +0.1 176,327 + gspF SO0168 0.28 -3.0 176,335 - gspF SO0168 0.29 -1.7 176,382 - gspF SO0168 0.33 -0.5 176,382 - gspF SO0168 0.33 -0.2 176,524 - gspF SO0168 0.44 -2.0 176,555 - gspF SO0168 0.47 -0.5 176,597 - gspF SO0168 0.50 -2.5 176,640 - gspF SO0168 0.54 -2.5 176,795 - gspF SO0168 0.67 -3.0 176,834 + gspF SO0168 0.70 -0.9 176,834 + gspF SO0168 0.70 +0.1 176,936 + gspF SO0168 0.78 -0.8 176,941 + gspF SO0168 0.78 -1.7 176,941 + gspF SO0168 0.78 -2.2 176,941 + gspF SO0168 0.78 -1.1 176,953 + gspF SO0168 0.79 -1.7 176,958 + gspF SO0168 0.80 -0.9 176,958 + gspF SO0168 0.80 -2.9 176,966 - gspF SO0168 0.80 -2.2 176,966 - gspF SO0168 0.80 -2.2 177,010 - gspF SO0168 0.84 -2.5 177,118 + -1.5 177,269 + -0.2 177,277 - -1.6 177,303 + -2.8 177,303 + -2.4 177,329 + gspG SO0169 0.11 -2.6 177,364 - gspG SO0169 0.20 -2.0 177,366 - gspG SO0169 0.20 -2.4 177,397 - gspG SO0169 0.27 -1.4 177,476 + gspG SO0169 0.45 -3.7 177,492 + gspG SO0169 0.49 +1.1 177,554 + gspG SO0169 0.63 -1.9 177,613 - gspG SO0169 0.77 -1.2 177,638 + gspG SO0169 0.83 -1.0 177,662 + gspG SO0169 0.88 -0.8 177,751 - -2.9 177,788 + gspH SO0170 0.13 -1.2 177,837 + gspH SO0170 0.21 -2.7 177,837 + gspH SO0170 0.21 -0.9 177,837 + gspH SO0170 0.21 -1.1 177,845 - gspH SO0170 0.23 -1.9 177,845 - gspH SO0170 0.23 -2.8 178,065 - gspH SO0170 0.61 -1.8 178,221 - gspH SO0170 0.88 -1.4 178,237 + -1.4 178,266 - -2.7 178,282 + +0.1 178,440 + gspI SO0171 0.45 -3.1 178,516 - gspI SO0171 0.66 -1.5 178,605 - gspI SO0171 0.90 -0.1 178,654 + -1.9 178,654 + -0.9 178,654 + -2.1 178,654 + -1.9 178,654 + -1.3 178,654 + -1.3 178,662 - -2.9 178,662 - -1.1 178,700 + gspJ SO0172 0.10 +0.1
Or see this region's nucleotide sequence