Experiment: m.b. Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02540 and PGA1_c02550 are separated by 11 nucleotides PGA1_c02550 and PGA1_c02560 overlap by 1 nucleotides PGA1_c02560 and PGA1_c02570 overlap by 14 nucleotides
PGA1_c02540: PGA1_c02540 - Uncharacterized conserved protein, at 239,283 to 240,119
_c02540
PGA1_c02550: PGA1_c02550 - protocatechuate 3,4-dioxygenase alpha chain, at 240,131 to 240,775
_c02550
PGA1_c02560: PGA1_c02560 - protocatechuate 3,4-dioxygenase beta chain, at 240,775 to 241,500
_c02560
PGA1_c02570: PGA1_c02570 - putative 4-carboxymuconolactone decarboxylase PcaC, at 241,487 to 241,912
_c02570
Position (kb) 240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 239.217 kb on - strand at 239.217 kb on - strand at 239.242 kb on - strand at 239.259 kb on - strand at 239.259 kb on - strand at 239.264 kb on - strand at 239.271 kb on - strand at 239.313 kb on + strand at 239.331 kb on + strand at 239.371 kb on - strand, within PGA1_c02540 at 239.371 kb on - strand, within PGA1_c02540 at 239.374 kb on + strand, within PGA1_c02540 at 239.548 kb on + strand, within PGA1_c02540 at 239.548 kb on + strand, within PGA1_c02540 at 239.637 kb on - strand, within PGA1_c02540 at 239.724 kb on - strand, within PGA1_c02540 at 239.729 kb on - strand, within PGA1_c02540 at 239.807 kb on - strand, within PGA1_c02540 at 239.866 kb on + strand, within PGA1_c02540 at 239.866 kb on + strand, within PGA1_c02540 at 239.883 kb on + strand, within PGA1_c02540 at 239.883 kb on + strand, within PGA1_c02540 at 239.888 kb on + strand, within PGA1_c02540 at 239.898 kb on + strand, within PGA1_c02540 at 239.906 kb on - strand, within PGA1_c02540 at 239.974 kb on - strand, within PGA1_c02540 at 240.014 kb on - strand, within PGA1_c02540 at 240.019 kb on + strand, within PGA1_c02540 at 240.032 kb on + strand, within PGA1_c02540 at 240.069 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.074 kb on + strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.082 kb on - strand at 240.117 kb on - strand at 240.154 kb on + strand at 240.164 kb on + strand at 240.196 kb on - strand, within PGA1_c02550 at 240.196 kb on - strand, within PGA1_c02550 at 240.197 kb on - strand, within PGA1_c02550 at 240.219 kb on + strand, within PGA1_c02550 at 240.237 kb on - strand, within PGA1_c02550 at 240.241 kb on + strand, within PGA1_c02550 at 240.244 kb on - strand, within PGA1_c02550 at 240.246 kb on + strand, within PGA1_c02550 at 240.254 kb on - strand, within PGA1_c02550 at 240.296 kb on - strand, within PGA1_c02550 at 240.320 kb on + strand, within PGA1_c02550 at 240.337 kb on + strand, within PGA1_c02550 at 240.337 kb on + strand, within PGA1_c02550 at 240.343 kb on + strand, within PGA1_c02550 at 240.343 kb on + strand, within PGA1_c02550 at 240.345 kb on - strand, within PGA1_c02550 at 240.351 kb on - strand, within PGA1_c02550 at 240.351 kb on - strand, within PGA1_c02550 at 240.363 kb on + strand, within PGA1_c02550 at 240.363 kb on + strand, within PGA1_c02550 at 240.565 kb on + strand, within PGA1_c02550 at 240.605 kb on - strand, within PGA1_c02550 at 240.613 kb on + strand, within PGA1_c02550 at 240.613 kb on - strand, within PGA1_c02550 at 240.643 kb on - strand, within PGA1_c02550 at 240.650 kb on - strand, within PGA1_c02550 at 240.665 kb on + strand, within PGA1_c02550 at 240.700 kb on - strand, within PGA1_c02550 at 240.720 kb on - strand at 240.736 kb on + strand at 240.736 kb on + strand at 240.737 kb on - strand at 240.744 kb on - strand at 240.786 kb on - strand at 240.812 kb on - strand at 240.892 kb on + strand, within PGA1_c02560 at 240.897 kb on + strand, within PGA1_c02560 at 240.903 kb on - strand, within PGA1_c02560 at 240.938 kb on - strand, within PGA1_c02560 at 240.973 kb on - strand, within PGA1_c02560 at 240.975 kb on + strand, within PGA1_c02560 at 240.983 kb on - strand, within PGA1_c02560 at 240.990 kb on + strand, within PGA1_c02560 at 240.998 kb on - strand, within PGA1_c02560 at 240.998 kb on - strand, within PGA1_c02560 at 241.036 kb on - strand, within PGA1_c02560 at 241.042 kb on + strand, within PGA1_c02560 at 241.061 kb on + strand, within PGA1_c02560 at 241.061 kb on + strand, within PGA1_c02560 at 241.061 kb on + strand, within PGA1_c02560 at 241.067 kb on + strand, within PGA1_c02560 at 241.067 kb on + strand, within PGA1_c02560 at 241.069 kb on - strand, within PGA1_c02560 at 241.069 kb on - strand, within PGA1_c02560 at 241.069 kb on - strand, within PGA1_c02560 at 241.069 kb on - strand, within PGA1_c02560 at 241.069 kb on - strand, within PGA1_c02560 at 241.075 kb on - strand, within PGA1_c02560 at 241.082 kb on - strand, within PGA1_c02560 at 241.082 kb on - strand, within PGA1_c02560 at 241.112 kb on + strand, within PGA1_c02560 at 241.112 kb on + strand, within PGA1_c02560 at 241.119 kb on + strand, within PGA1_c02560 at 241.143 kb on + strand, within PGA1_c02560 at 241.227 kb on + strand, within PGA1_c02560 at 241.230 kb on - strand, within PGA1_c02560 at 241.240 kb on - strand, within PGA1_c02560 at 241.340 kb on + strand, within PGA1_c02560 at 241.348 kb on - strand, within PGA1_c02560 at 241.362 kb on + strand, within PGA1_c02560 at 241.365 kb on - strand, within PGA1_c02560 at 241.382 kb on + strand, within PGA1_c02560 at 241.386 kb on + strand, within PGA1_c02560 at 241.386 kb on + strand, within PGA1_c02560 at 241.399 kb on - strand, within PGA1_c02560 at 241.419 kb on + strand, within PGA1_c02560 at 241.503 kb on - strand at 241.518 kb on - strand at 241.597 kb on + strand, within PGA1_c02570 at 241.601 kb on + strand, within PGA1_c02570 at 241.605 kb on - strand, within PGA1_c02570 at 241.618 kb on + strand, within PGA1_c02570 at 241.645 kb on - strand, within PGA1_c02570 at 241.647 kb on - strand, within PGA1_c02570 at 241.658 kb on + strand, within PGA1_c02570 at 241.676 kb on - strand, within PGA1_c02570 at 241.746 kb on + strand, within PGA1_c02570 at 241.746 kb on + strand, within PGA1_c02570
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1 mM remove 239,217 - +0.3 239,217 - +0.1 239,242 - +1.6 239,259 - -0.2 239,259 - -0.8 239,264 - +0.4 239,271 - +0.5 239,313 + +0.7 239,331 + +0.7 239,371 - PGA1_c02540 0.11 -0.4 239,371 - PGA1_c02540 0.11 -0.6 239,374 + PGA1_c02540 0.11 +1.4 239,548 + PGA1_c02540 0.32 -0.0 239,548 + PGA1_c02540 0.32 -0.6 239,637 - PGA1_c02540 0.42 +0.4 239,724 - PGA1_c02540 0.53 +0.2 239,729 - PGA1_c02540 0.53 -0.5 239,807 - PGA1_c02540 0.63 +0.6 239,866 + PGA1_c02540 0.70 -0.8 239,866 + PGA1_c02540 0.70 -1.0 239,883 + PGA1_c02540 0.72 +0.1 239,883 + PGA1_c02540 0.72 -0.6 239,888 + PGA1_c02540 0.72 -0.0 239,898 + PGA1_c02540 0.73 -0.4 239,906 - PGA1_c02540 0.74 -1.5 239,974 - PGA1_c02540 0.83 +0.8 240,014 - PGA1_c02540 0.87 +1.2 240,019 + PGA1_c02540 0.88 -0.3 240,032 + PGA1_c02540 0.89 +0.2 240,069 + +1.0 240,074 + -1.2 240,074 + +0.3 240,074 + -0.4 240,074 + -0.1 240,074 + +1.5 240,074 + -0.3 240,074 + +0.3 240,074 + -2.3 240,082 - +0.4 240,082 - -0.0 240,082 - +0.2 240,082 - +0.4 240,082 - +0.2 240,082 - -1.3 240,082 - +0.1 240,082 - +0.7 240,082 - +0.0 240,117 - +0.6 240,154 + -0.9 240,164 + +1.9 240,196 - PGA1_c02550 0.10 +0.9 240,196 - PGA1_c02550 0.10 -0.4 240,197 - PGA1_c02550 0.10 +1.1 240,219 + PGA1_c02550 0.14 +0.4 240,237 - PGA1_c02550 0.16 +0.0 240,241 + PGA1_c02550 0.17 +0.0 240,244 - PGA1_c02550 0.18 +1.2 240,246 + PGA1_c02550 0.18 +1.1 240,254 - PGA1_c02550 0.19 +0.7 240,296 - PGA1_c02550 0.26 +0.9 240,320 + PGA1_c02550 0.29 -2.3 240,337 + PGA1_c02550 0.32 +0.7 240,337 + PGA1_c02550 0.32 +1.4 240,343 + PGA1_c02550 0.33 -0.3 240,343 + PGA1_c02550 0.33 +0.9 240,345 - PGA1_c02550 0.33 +0.4 240,351 - PGA1_c02550 0.34 +0.3 240,351 - PGA1_c02550 0.34 -1.3 240,363 + PGA1_c02550 0.36 +0.8 240,363 + PGA1_c02550 0.36 -0.5 240,565 + PGA1_c02550 0.67 +0.4 240,605 - PGA1_c02550 0.73 +1.1 240,613 + PGA1_c02550 0.75 -1.0 240,613 - PGA1_c02550 0.75 +0.5 240,643 - PGA1_c02550 0.79 +1.4 240,650 - PGA1_c02550 0.80 +0.7 240,665 + PGA1_c02550 0.83 +0.4 240,700 - PGA1_c02550 0.88 -1.7 240,720 - -2.0 240,736 + -2.4 240,736 + +0.1 240,737 - +1.2 240,744 - -0.8 240,786 - +0.4 240,812 - +0.3 240,892 + PGA1_c02560 0.16 +0.9 240,897 + PGA1_c02560 0.17 +0.2 240,903 - PGA1_c02560 0.18 -0.6 240,938 - PGA1_c02560 0.22 -0.9 240,973 - PGA1_c02560 0.27 +0.2 240,975 + PGA1_c02560 0.28 +0.6 240,983 - PGA1_c02560 0.29 +1.1 240,990 + PGA1_c02560 0.30 -0.7 240,998 - PGA1_c02560 0.31 +0.1 240,998 - PGA1_c02560 0.31 +2.0 241,036 - PGA1_c02560 0.36 +0.3 241,042 + PGA1_c02560 0.37 -0.5 241,061 + PGA1_c02560 0.39 -0.3 241,061 + PGA1_c02560 0.39 +0.8 241,061 + PGA1_c02560 0.39 -1.2 241,067 + PGA1_c02560 0.40 -0.5 241,067 + PGA1_c02560 0.40 -0.3 241,069 - PGA1_c02560 0.40 -0.1 241,069 - PGA1_c02560 0.40 -0.6 241,069 - PGA1_c02560 0.40 +0.7 241,069 - PGA1_c02560 0.40 -0.3 241,069 - PGA1_c02560 0.40 -0.9 241,075 - PGA1_c02560 0.41 -2.8 241,082 - PGA1_c02560 0.42 -1.7 241,082 - PGA1_c02560 0.42 -1.1 241,112 + PGA1_c02560 0.46 +1.0 241,112 + PGA1_c02560 0.46 -0.3 241,119 + PGA1_c02560 0.47 +0.6 241,143 + PGA1_c02560 0.51 +0.2 241,227 + PGA1_c02560 0.62 +0.9 241,230 - PGA1_c02560 0.63 +0.2 241,240 - PGA1_c02560 0.64 -0.3 241,340 + PGA1_c02560 0.78 -0.1 241,348 - PGA1_c02560 0.79 +0.5 241,362 + PGA1_c02560 0.81 +0.1 241,365 - PGA1_c02560 0.81 -0.1 241,382 + PGA1_c02560 0.84 -0.0 241,386 + PGA1_c02560 0.84 -0.5 241,386 + PGA1_c02560 0.84 +0.8 241,399 - PGA1_c02560 0.86 +1.3 241,419 + PGA1_c02560 0.89 +0.7 241,503 - +1.5 241,518 - -0.5 241,597 + PGA1_c02570 0.26 +2.3 241,601 + PGA1_c02570 0.27 +0.5 241,605 - PGA1_c02570 0.28 -0.8 241,618 + PGA1_c02570 0.31 -0.3 241,645 - PGA1_c02570 0.37 -2.0 241,647 - PGA1_c02570 0.38 -0.3 241,658 + PGA1_c02570 0.40 +0.9 241,676 - PGA1_c02570 0.44 -1.0 241,746 + PGA1_c02570 0.61 -0.3 241,746 + PGA1_c02570 0.61 -0.2
Or see this region's nucleotide sequence