Strain Fitness in Shewanella oneidensis MR-1 around SO3294

Experiment: Lomefloxacin 0.0002 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntguaB and xseA are separated by 122 nucleotidesxseA and SO3296 are separated by 116 nucleotides SO3293: guaB - inosine-5'-monophosphate dehydrogenase (NCBI ptt file), at 3,437,369 to 3,438,835 guaB SO3294: xseA - exodeoxyribonuclease VII, large subunit (NCBI ptt file), at 3,438,958 to 3,440,295 xseA SO3296: SO3296 - ISSod12, transposase (NCBI ptt file), at 3,440,412 to 3,441,335 SO3296 Position (kb) 3438 3439 3440 3441Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3437.967 kb on + strandat 3438.044 kb on + strand, within guaBat 3438.117 kb on + strandat 3438.117 kb on + strand, within guaBat 3438.125 kb on - strand, within guaBat 3438.148 kb on - strand, within guaBat 3438.188 kb on + strand, within guaBat 3438.236 kb on + strandat 3438.244 kb on - strand, within guaBat 3438.248 kb on + strand, within guaBat 3438.256 kb on - strand, within guaBat 3438.429 kb on - strand, within guaBat 3438.532 kb on - strand, within guaBat 3438.731 kb on + strandat 3438.766 kb on - strandat 3438.766 kb on - strandat 3438.887 kb on + strandat 3438.887 kb on + strandat 3438.887 kb on + strandat 3438.895 kb on - strandat 3438.895 kb on - strandat 3438.895 kb on - strandat 3438.996 kb on + strandat 3439.082 kb on + strandat 3439.167 kb on + strand, within xseAat 3439.277 kb on + strand, within xseAat 3439.283 kb on - strand, within xseAat 3439.285 kb on - strand, within xseAat 3439.392 kb on - strand, within xseAat 3439.403 kb on - strand, within xseAat 3439.429 kb on - strand, within xseAat 3439.438 kb on - strand, within xseAat 3439.566 kb on - strand, within xseAat 3439.671 kb on - strand, within xseAat 3439.779 kb on - strand, within xseAat 3439.779 kb on - strand, within xseAat 3439.791 kb on - strand, within xseAat 3439.801 kb on - strand, within xseAat 3439.830 kb on + strand, within xseAat 3439.830 kb on + strand, within xseAat 3439.937 kb on - strand, within xseAat 3439.937 kb on - strand, within xseAat 3439.961 kb on + strand, within xseAat 3440.002 kb on - strand, within xseAat 3440.087 kb on + strand, within xseAat 3440.090 kb on - strand, within xseAat 3440.095 kb on - strand, within xseAat 3440.130 kb on + strand, within xseAat 3440.168 kb on - strandat 3440.183 kb on - strandat 3440.233 kb on - strandat 3440.251 kb on - strandat 3440.304 kb on - strandat 3440.353 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM
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3,437,967 + -0.5
3,438,044 + guaB SO3293 0.46 -0.6
3,438,117 + +0.5
3,438,117 + guaB SO3293 0.51 +0.4
3,438,125 - guaB SO3293 0.52 -1.6
3,438,148 - guaB SO3293 0.53 +0.1
3,438,188 + guaB SO3293 0.56 -0.1
3,438,236 + +0.2
3,438,244 - guaB SO3293 0.60 -1.0
3,438,248 + guaB SO3293 0.60 -0.3
3,438,256 - guaB SO3293 0.60 -1.6
3,438,429 - guaB SO3293 0.72 +1.8
3,438,532 - guaB SO3293 0.79 +1.8
3,438,731 + -0.3
3,438,766 - +2.1
3,438,766 - +1.0
3,438,887 + -0.2
3,438,887 + -1.4
3,438,887 + +0.7
3,438,895 - -1.8
3,438,895 - -1.1
3,438,895 - -0.3
3,438,996 + -2.9
3,439,082 + -4.1
3,439,167 + xseA SO3294 0.16 -2.6
3,439,277 + xseA SO3294 0.24 -3.9
3,439,283 - xseA SO3294 0.24 -4.3
3,439,285 - xseA SO3294 0.24 -2.6
3,439,392 - xseA SO3294 0.32 -2.3
3,439,403 - xseA SO3294 0.33 -3.8
3,439,429 - xseA SO3294 0.35 -4.5
3,439,438 - xseA SO3294 0.36 -4.5
3,439,566 - xseA SO3294 0.45 -1.5
3,439,671 - xseA SO3294 0.53 -0.2
3,439,779 - xseA SO3294 0.61 -4.0
3,439,779 - xseA SO3294 0.61 -4.4
3,439,791 - xseA SO3294 0.62 -1.9
3,439,801 - xseA SO3294 0.63 -2.9
3,439,830 + xseA SO3294 0.65 -3.7
3,439,830 + xseA SO3294 0.65 -3.4
3,439,937 - xseA SO3294 0.73 -4.3
3,439,937 - xseA SO3294 0.73 -2.1
3,439,961 + xseA SO3294 0.75 -0.1
3,440,002 - xseA SO3294 0.78 -3.4
3,440,087 + xseA SO3294 0.84 -3.9
3,440,090 - xseA SO3294 0.85 -4.7
3,440,095 - xseA SO3294 0.85 -2.4
3,440,130 + xseA SO3294 0.88 -4.7
3,440,168 - -2.9
3,440,183 - -4.9
3,440,233 - -1.9
3,440,251 - -1.7
3,440,304 - -0.0
3,440,353 - +1.4

Or see this region's nucleotide sequence