Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chiA and SO4087 are separated by 159 nucleotides SO4087 and SO4088 are separated by 1 nucleotides
SO4085: chiA - chitinase A (NCBI ptt file), at 4,238,538 to 4,240,637
chiA
SO4087: SO4087 - conserved hypothetical protein (NCBI ptt file), at 4,240,797 to 4,241,933
SO4087
SO4088: SO4088 - conserved hypothetical protein (NCBI ptt file), at 4,241,935 to 4,243,242
SO4088
Position (kb)
4240
4241
4242 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4239.949 kb on - strand, within chiA at 4239.956 kb on + strand, within chiA at 4240.045 kb on - strand, within chiA at 4240.129 kb on - strand, within chiA at 4240.182 kb on - strand, within chiA at 4240.270 kb on + strand, within chiA at 4240.285 kb on - strand, within chiA at 4240.415 kb on + strand, within chiA at 4240.417 kb on + strand, within chiA at 4240.423 kb on - strand, within chiA at 4240.423 kb on - strand, within chiA at 4240.423 kb on - strand, within chiA at 4240.552 kb on - strand at 4240.631 kb on + strand at 4240.643 kb on + strand at 4240.796 kb on + strand at 4240.801 kb on + strand at 4240.801 kb on + strand at 4240.826 kb on - strand at 4240.982 kb on - strand, within SO4087 at 4241.174 kb on + strand, within SO4087 at 4241.182 kb on - strand, within SO4087 at 4241.182 kb on - strand, within SO4087 at 4241.209 kb on - strand, within SO4087 at 4241.276 kb on + strand, within SO4087 at 4241.380 kb on - strand, within SO4087 at 4241.543 kb on + strand, within SO4087 at 4241.847 kb on - strand at 4241.864 kb on + strand at 4241.864 kb on + strand at 4241.864 kb on + strand at 4241.872 kb on - strand at 4241.990 kb on + strand at 4242.086 kb on - strand, within SO4088 at 4242.222 kb on - strand, within SO4088 at 4242.350 kb on - strand, within SO4088 at 4242.350 kb on - strand, within SO4088 at 4242.350 kb on - strand, within SO4088 at 4242.350 kb on - strand, within SO4088 at 4242.350 kb on - strand, within SO4088 at 4242.350 kb on - strand, within SO4088 at 4242.366 kb on + strand, within SO4088 at 4242.448 kb on + strand, within SO4088 at 4242.458 kb on - strand, within SO4088 at 4242.458 kb on - strand, within SO4088 at 4242.506 kb on + strand, within SO4088 at 4242.514 kb on - strand, within SO4088 at 4242.514 kb on - strand, within SO4088 at 4242.560 kb on + strand, within SO4088 at 4242.627 kb on + strand, within SO4088 at 4242.648 kb on - strand, within SO4088 at 4242.863 kb on + strand, within SO4088 at 4242.863 kb on + strand, within SO4088 at 4242.864 kb on + strand, within SO4088 at 4242.871 kb on - strand, within SO4088 at 4242.871 kb on - strand, within SO4088 at 4242.871 kb on - strand, within SO4088 at 4242.876 kb on - strand, within SO4088 at 4242.929 kb on + strand, within SO4088
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 4,239,949 - chiA SO4085 0.67 -0.2 4,239,956 + chiA SO4085 0.68 -1.3 4,240,045 - chiA SO4085 0.72 +0.4 4,240,129 - chiA SO4085 0.76 -0.5 4,240,182 - chiA SO4085 0.78 +1.2 4,240,270 + chiA SO4085 0.82 -0.4 4,240,285 - chiA SO4085 0.83 +1.2 4,240,415 + chiA SO4085 0.89 +0.0 4,240,417 + chiA SO4085 0.89 -0.3 4,240,423 - chiA SO4085 0.90 +0.7 4,240,423 - chiA SO4085 0.90 -0.9 4,240,423 - chiA SO4085 0.90 -0.2 4,240,552 - -0.6 4,240,631 + +0.4 4,240,643 + -0.1 4,240,796 + -0.3 4,240,801 + +0.2 4,240,801 + +0.0 4,240,826 - -0.2 4,240,982 - SO4087 0.16 -2.7 4,241,174 + SO4087 0.33 -1.6 4,241,182 - SO4087 0.34 -1.5 4,241,182 - SO4087 0.34 -3.9 4,241,209 - SO4087 0.36 -3.0 4,241,276 + SO4087 0.42 -4.3 4,241,380 - SO4087 0.51 -3.9 4,241,543 + SO4087 0.66 -4.6 4,241,847 - -5.2 4,241,864 + -3.3 4,241,864 + -1.4 4,241,864 + -5.6 4,241,872 - -4.3 4,241,990 + -5.2 4,242,086 - SO4088 0.12 -4.0 4,242,222 - SO4088 0.22 -5.0 4,242,350 - SO4088 0.32 -2.4 4,242,350 - SO4088 0.32 -2.9 4,242,350 - SO4088 0.32 -2.3 4,242,350 - SO4088 0.32 -0.2 4,242,350 - SO4088 0.32 -5.3 4,242,350 - SO4088 0.32 -4.5 4,242,366 + SO4088 0.33 -3.8 4,242,448 + SO4088 0.39 -2.6 4,242,458 - SO4088 0.40 -4.3 4,242,458 - SO4088 0.40 -4.3 4,242,506 + SO4088 0.44 -4.0 4,242,514 - SO4088 0.44 -3.7 4,242,514 - SO4088 0.44 -1.1 4,242,560 + SO4088 0.48 -5.1 4,242,627 + SO4088 0.53 -5.7 4,242,648 - SO4088 0.55 -3.2 4,242,863 + SO4088 0.71 -5.8 4,242,863 + SO4088 0.71 -2.6 4,242,864 + SO4088 0.71 -5.1 4,242,871 - SO4088 0.72 -3.4 4,242,871 - SO4088 0.72 -4.5 4,242,871 - SO4088 0.72 -2.6 4,242,876 - SO4088 0.72 -3.1 4,242,929 + SO4088 0.76 -4.3
Or see this region's nucleotide sequence