Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD-2 and yajC are separated by 17 nucleotides yajC and tgt are separated by 26 nucleotides tgt and queA are separated by 161 nucleotides
SO3111: secD-2 - protein-export membrane protein SecD (NCBI ptt file), at 3,239,490 to 3,241,340
secD-2
SO3112: yajC - preprotein translocase, YajC subunit (NCBI ptt file), at 3,241,358 to 3,241,690
yajC
SO3113: tgt - queuine tRNA-ribosyltransferase (NCBI ptt file), at 3,241,717 to 3,242,841
tgt
SO3114: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 3,243,003 to 3,244,040
queA
Position (kb)
3241
3242
3243 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3240.750 kb on - strand, within secD-2 at 3240.751 kb on - strand, within secD-2 at 3241.007 kb on + strand, within secD-2 at 3241.153 kb on + strand, within secD-2 at 3241.175 kb on - strand at 3241.196 kb on - strand at 3241.214 kb on - strand at 3241.342 kb on + strand at 3241.350 kb on - strand at 3241.350 kb on - strand at 3241.377 kb on - strand at 3241.651 kb on - strand, within yajC at 3241.651 kb on - strand, within yajC at 3241.913 kb on - strand, within tgt at 3242.050 kb on + strand, within tgt at 3242.071 kb on + strand, within tgt at 3242.236 kb on - strand, within tgt at 3242.286 kb on - strand, within tgt at 3242.320 kb on + strand, within tgt at 3242.345 kb on + strand, within tgt at 3242.364 kb on + strand, within tgt at 3242.427 kb on + strand, within tgt at 3242.663 kb on - strand, within tgt at 3242.669 kb on + strand, within tgt at 3242.669 kb on + strand, within tgt at 3242.677 kb on - strand, within tgt at 3242.722 kb on - strand, within tgt at 3242.724 kb on - strand, within tgt at 3242.964 kb on + strand at 3242.964 kb on + strand at 3243.226 kb on + strand, within queA at 3243.226 kb on + strand, within queA at 3243.260 kb on - strand, within queA at 3243.353 kb on + strand, within queA at 3243.545 kb on - strand, within queA at 3243.568 kb on - strand, within queA at 3243.612 kb on - strand, within queA at 3243.627 kb on - strand, within queA at 3243.629 kb on + strand, within queA at 3243.751 kb on - strand, within queA at 3243.766 kb on + strand, within queA at 3243.825 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 3,240,750 - secD-2 SO3111 0.68 -0.7 3,240,751 - secD-2 SO3111 0.68 +0.0 3,241,007 + secD-2 SO3111 0.82 -1.3 3,241,153 + secD-2 SO3111 0.90 -2.6 3,241,175 - -1.1 3,241,196 - -0.7 3,241,214 - -1.0 3,241,342 + -0.9 3,241,350 - -1.9 3,241,350 - -2.3 3,241,377 - -2.2 3,241,651 - yajC SO3112 0.88 -2.7 3,241,651 - yajC SO3112 0.88 -4.0 3,241,913 - tgt SO3113 0.17 -2.8 3,242,050 + tgt SO3113 0.30 -2.5 3,242,071 + tgt SO3113 0.31 -3.2 3,242,236 - tgt SO3113 0.46 -2.3 3,242,286 - tgt SO3113 0.51 -1.4 3,242,320 + tgt SO3113 0.54 -3.7 3,242,345 + tgt SO3113 0.56 -1.4 3,242,364 + tgt SO3113 0.58 -1.9 3,242,427 + tgt SO3113 0.63 -3.8 3,242,663 - tgt SO3113 0.84 -2.5 3,242,669 + tgt SO3113 0.85 -3.5 3,242,669 + tgt SO3113 0.85 -4.1 3,242,677 - tgt SO3113 0.85 -1.1 3,242,722 - tgt SO3113 0.89 -1.2 3,242,724 - tgt SO3113 0.90 +2.3 3,242,964 + -1.4 3,242,964 + -1.0 3,243,226 + queA SO3114 0.21 -0.2 3,243,226 + queA SO3114 0.21 -0.9 3,243,260 - queA SO3114 0.25 +1.9 3,243,353 + queA SO3114 0.34 -0.1 3,243,545 - queA SO3114 0.52 -0.3 3,243,568 - queA SO3114 0.54 +1.3 3,243,612 - queA SO3114 0.59 -0.3 3,243,627 - queA SO3114 0.60 -0.2 3,243,629 + queA SO3114 0.60 +0.6 3,243,751 - queA SO3114 0.72 -0.7 3,243,766 + queA SO3114 0.74 -0.3 3,243,825 - queA SO3114 0.79 +0.3
Or see this region's nucleotide sequence