Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2571 and ppiB-2 are separated by 24 nucleotides ppiB-2 and SO2573 are separated by 187 nucleotides
SO2571: SO2571 - ATP-dependent RNA helicase, DEAD box family (NCBI ptt file), at 2,701,334 to 2,702,761
SO2571
SO2572: ppiB-2 - peptidyl-prolyl cis-trans isomerase B (NCBI ptt file), at 2,702,786 to 2,703,280
ppiB-2
SO2573: SO2573 - conserved hypothetical protein (NCBI ptt file), at 2,703,468 to 2,703,908
SO2573
Position (kb)
2702
2703
2704 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2701.792 kb on + strand, within SO2571 at 2701.914 kb on - strand, within SO2571 at 2701.918 kb on + strand, within SO2571 at 2701.918 kb on + strand, within SO2571 at 2702.061 kb on - strand, within SO2571 at 2702.159 kb on + strand, within SO2571 at 2702.159 kb on + strand, within SO2571 at 2702.252 kb on + strand, within SO2571 at 2702.340 kb on - strand, within SO2571 at 2702.507 kb on + strand, within SO2571 at 2702.589 kb on + strand, within SO2571 at 2702.658 kb on + strand at 2702.834 kb on - strand at 2703.056 kb on - strand, within ppiB-2 at 2703.147 kb on - strand, within ppiB-2 at 2703.199 kb on + strand, within ppiB-2 at 2703.199 kb on + strand, within ppiB-2 at 2703.199 kb on + strand, within ppiB-2 at 2703.230 kb on + strand, within ppiB-2 at 2703.309 kb on - strand at 2703.311 kb on + strand at 2703.316 kb on + strand at 2703.319 kb on - strand at 2703.319 kb on - strand at 2703.515 kb on + strand, within SO2573 at 2703.515 kb on + strand, within SO2573 at 2703.515 kb on + strand, within SO2573 at 2703.515 kb on + strand, within SO2573 at 2703.621 kb on + strand, within SO2573 at 2703.707 kb on - strand, within SO2573 at 2703.792 kb on - strand, within SO2573 at 2703.871 kb on - strand at 2703.973 kb on + strand at 2704.189 kb on + strand at 2704.197 kb on - strand at 2704.197 kb on - strand at 2704.197 kb on - strand at 2704.253 kb on + strand at 2704.253 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 2,701,792 + SO2571 0.32 +0.2 2,701,914 - SO2571 0.41 +0.1 2,701,918 + SO2571 0.41 -0.1 2,701,918 + SO2571 0.41 -1.7 2,702,061 - SO2571 0.51 -1.6 2,702,159 + SO2571 0.58 -0.6 2,702,159 + SO2571 0.58 -0.2 2,702,252 + SO2571 0.64 -1.1 2,702,340 - SO2571 0.70 -1.1 2,702,507 + SO2571 0.82 -0.3 2,702,589 + SO2571 0.88 +0.7 2,702,658 + -0.5 2,702,834 - -0.1 2,703,056 - ppiB-2 SO2572 0.55 -0.4 2,703,147 - ppiB-2 SO2572 0.73 +0.5 2,703,199 + ppiB-2 SO2572 0.83 -0.3 2,703,199 + ppiB-2 SO2572 0.83 +0.1 2,703,199 + ppiB-2 SO2572 0.83 +0.5 2,703,230 + ppiB-2 SO2572 0.90 +0.3 2,703,309 - -0.3 2,703,311 + +0.4 2,703,316 + -0.4 2,703,319 - -0.0 2,703,319 - +0.5 2,703,515 + SO2573 0.11 +1.7 2,703,515 + SO2573 0.11 -0.1 2,703,515 + SO2573 0.11 -0.9 2,703,515 + SO2573 0.11 +0.9 2,703,621 + SO2573 0.35 -0.0 2,703,707 - SO2573 0.54 +0.2 2,703,792 - SO2573 0.73 -0.3 2,703,871 - +0.7 2,703,973 + -0.0 2,704,189 + -0.6 2,704,197 - +0.1 2,704,197 - +1.1 2,704,197 - +1.9 2,704,253 + -2.4 2,704,253 + -1.2
Or see this region's nucleotide sequence