Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recO and pdxJ are separated by 84 nucleotides pdxJ and acpS overlap by 1 nucleotides acpS and SO1353 are separated by 170 nucleotides SO1353 and SO1354 are separated by 67 nucleotides
SO1350: recO - DNA repair protein RecO (NCBI ptt file), at 1,406,677 to 1,407,378
recO
SO1351: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ (NCBI ptt file), at 1,407,463 to 1,408,200
pdxJ
SO1352: acpS - holo-(acyl-carrier protein) synthase (NCBI ptt file), at 1,408,200 to 1,408,583
acpS
SO1353: SO1353 - conserved hypothetical protein (NCBI ptt file), at 1,408,754 to 1,409,281
SO1353
SO1354: SO1354 - hemolysin protein, putative (NCBI ptt file), at 1,409,349 to 1,410,620
SO1354
Position (kb)
1408
1409 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1407.314 kb on + strand at 1407.360 kb on - strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.410 kb on + strand at 1407.418 kb on - strand at 1408.297 kb on - strand, within acpS at 1408.490 kb on - strand, within acpS at 1408.599 kb on + strand at 1408.736 kb on + strand at 1408.922 kb on + strand, within SO1353 at 1409.008 kb on + strand, within SO1353 at 1409.010 kb on + strand, within SO1353 at 1409.021 kb on - strand, within SO1353 at 1409.021 kb on - strand, within SO1353 at 1409.121 kb on - strand, within SO1353 at 1409.217 kb on - strand, within SO1353 at 1409.362 kb on + strand at 1409.479 kb on - strand, within SO1354 at 1409.511 kb on - strand, within SO1354 at 1409.583 kb on + strand, within SO1354
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 1,407,314 + +0.4 1,407,360 - -1.8 1,407,410 + +0.7 1,407,410 + +0.4 1,407,410 + +0.2 1,407,410 + +0.7 1,407,418 - -0.1 1,408,297 - acpS SO1352 0.25 -0.2 1,408,490 - acpS SO1352 0.76 +2.3 1,408,599 + -0.9 1,408,736 + -0.6 1,408,922 + SO1353 0.32 +0.1 1,409,008 + SO1353 0.48 -0.8 1,409,010 + SO1353 0.48 +1.0 1,409,021 - SO1353 0.51 +1.3 1,409,021 - SO1353 0.51 +0.9 1,409,121 - SO1353 0.70 +1.0 1,409,217 - SO1353 0.88 -0.1 1,409,362 + -0.9 1,409,479 - SO1354 0.10 -0.6 1,409,511 - SO1354 0.13 -0.1 1,409,583 + SO1354 0.18 -0.1
Or see this region's nucleotide sequence