Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and mutL are separated by 13 nucleotides mutL and miaA overlap by 8 nucleotides
SO0600: amiB - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 625,445 to 626,836
amiB
SO0601: mutL - DNA mismatch repair protein MutL (NCBI ptt file), at 626,850 to 628,745
mutL
SO0602: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 628,738 to 629,664
miaA
Position (kb) 626
627
628
629 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 625.865 kb on - strand, within amiB at 625.886 kb on - strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.364 kb on + strand, within amiB at 626.431 kb on + strand, within amiB at 626.663 kb on + strand, within amiB at 626.898 kb on + strand at 626.920 kb on + strand at 626.958 kb on - strand at 626.964 kb on - strand at 626.998 kb on + strand at 626.998 kb on + strand at 627.062 kb on - strand, within mutL at 627.102 kb on - strand, within mutL at 627.104 kb on + strand, within mutL at 627.112 kb on - strand, within mutL at 627.190 kb on + strand, within mutL at 627.190 kb on + strand, within mutL at 627.198 kb on - strand, within mutL at 627.200 kb on + strand, within mutL at 627.280 kb on + strand, within mutL at 627.370 kb on + strand, within mutL at 627.384 kb on + strand, within mutL at 627.546 kb on + strand, within mutL at 627.588 kb on + strand, within mutL at 627.588 kb on + strand, within mutL at 627.588 kb on - strand, within mutL at 627.708 kb on - strand, within mutL at 627.912 kb on - strand, within mutL at 627.972 kb on + strand, within mutL at 627.972 kb on + strand, within mutL at 627.975 kb on + strand, within mutL at 627.978 kb on - strand, within mutL at 628.050 kb on + strand, within mutL at 628.070 kb on - strand, within mutL at 628.126 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.212 kb on - strand, within mutL at 628.529 kb on + strand, within mutL at 628.537 kb on - strand, within mutL at 628.537 kb on - strand, within mutL at 628.695 kb on + strand at 628.891 kb on + strand, within miaA at 629.010 kb on + strand, within miaA at 629.302 kb on + strand, within miaA at 629.395 kb on + strand, within miaA at 629.471 kb on + strand, within miaA
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 625,865 - amiB SO0600 0.30 -3.7 625,886 - amiB SO0600 0.32 -3.4 626,364 + amiB SO0600 0.66 -5.1 626,364 + amiB SO0600 0.66 -1.2 626,364 + amiB SO0600 0.66 -2.1 626,364 + amiB SO0600 0.66 -3.3 626,431 + amiB SO0600 0.71 -5.1 626,663 + amiB SO0600 0.88 -0.7 626,898 + -1.2 626,920 + +0.0 626,958 - -1.0 626,964 - +0.2 626,998 + -0.8 626,998 + -0.8 627,062 - mutL SO0601 0.11 -1.4 627,102 - mutL SO0601 0.13 -1.0 627,104 + mutL SO0601 0.13 -0.9 627,112 - mutL SO0601 0.14 -0.4 627,190 + mutL SO0601 0.18 -0.9 627,190 + mutL SO0601 0.18 -0.9 627,198 - mutL SO0601 0.18 -0.6 627,200 + mutL SO0601 0.18 +0.2 627,280 + mutL SO0601 0.23 -2.3 627,370 + mutL SO0601 0.27 -0.1 627,384 + mutL SO0601 0.28 +0.3 627,546 + mutL SO0601 0.37 -1.3 627,588 + mutL SO0601 0.39 +0.9 627,588 + mutL SO0601 0.39 +0.2 627,588 - mutL SO0601 0.39 -0.1 627,708 - mutL SO0601 0.45 -0.7 627,912 - mutL SO0601 0.56 -0.4 627,972 + mutL SO0601 0.59 +0.7 627,972 + mutL SO0601 0.59 -1.9 627,975 + mutL SO0601 0.59 -0.0 627,978 - mutL SO0601 0.59 -2.1 628,050 + mutL SO0601 0.63 -0.3 628,070 - mutL SO0601 0.64 -0.2 628,126 + mutL SO0601 0.67 +0.3 628,199 + mutL SO0601 0.71 -0.0 628,199 + mutL SO0601 0.71 -0.0 628,199 + mutL SO0601 0.71 -0.6 628,199 + mutL SO0601 0.71 -0.6 628,199 + mutL SO0601 0.71 -0.0 628,199 + mutL SO0601 0.71 -0.2 628,199 + mutL SO0601 0.71 -0.2 628,199 + mutL SO0601 0.71 +0.6 628,207 - mutL SO0601 0.72 -1.3 628,207 - mutL SO0601 0.72 -0.7 628,207 - mutL SO0601 0.72 -1.2 628,207 - mutL SO0601 0.72 -0.6 628,207 - mutL SO0601 0.72 -0.7 628,207 - mutL SO0601 0.72 -0.9 628,207 - mutL SO0601 0.72 -0.3 628,207 - mutL SO0601 0.72 -0.7 628,207 - mutL SO0601 0.72 -0.2 628,207 - mutL SO0601 0.72 -1.0 628,207 - mutL SO0601 0.72 -0.2 628,207 - mutL SO0601 0.72 -0.2 628,207 - mutL SO0601 0.72 -0.4 628,212 - mutL SO0601 0.72 -2.5 628,529 + mutL SO0601 0.89 -2.8 628,537 - mutL SO0601 0.89 -3.0 628,537 - mutL SO0601 0.89 -2.5 628,695 + -4.7 628,891 + miaA SO0602 0.17 -2.0 629,010 + miaA SO0602 0.29 -2.5 629,302 + miaA SO0602 0.61 -1.6 629,395 + miaA SO0602 0.71 -0.9 629,471 + miaA SO0602 0.79 -1.7
Or see this region's nucleotide sequence