Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0050 and SO0051 overlap by 18 nucleotides SO0051 and secB are separated by 186 nucleotides secB and gpsA are separated by 4 nucleotides gpsA and SO0054 are separated by 349 nucleotides
SO0050: SO0050 - rhodanese domain protein (NCBI ptt file), at 57,409 to 57,843
SO0050
SO0051: SO0051 - hypothetical protein (NCBI ptt file), at 57,826 to 57,918
SO0051
SO0052: secB - protein-export protein SecB (NCBI ptt file), at 58,105 to 58,578
secB
SO0053: gpsA - glycerol-3-phosphate dehydrogenase (NAD(P)+) (NCBI ptt file), at 58,583 to 59,599
gpsA
SO0054: SO0054 - conserved hypothetical protein TIGR00275 (NCBI ptt file), at 59,949 to 61,139
SO0054
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 57.695 kb on + strand, within SO0050 at 57.728 kb on + strand, within SO0050 at 57.811 kb on + strand at 57.893 kb on + strand, within SO0051 at 57.893 kb on + strand, within SO0051 at 57.979 kb on + strand at 57.979 kb on + strand at 57.989 kb on + strand at 58.027 kb on + strand at 58.027 kb on + strand at 58.027 kb on + strand at 58.027 kb on + strand at 59.635 kb on + strand at 59.709 kb on + strand at 59.709 kb on + strand at 59.709 kb on + strand at 59.796 kb on + strand at 59.796 kb on + strand at 59.804 kb on - strand at 59.804 kb on - strand at 59.804 kb on - strand at 59.883 kb on - strand at 59.945 kb on + strand at 59.945 kb on + strand at 59.953 kb on - strand at 59.961 kb on - strand at 59.961 kb on - strand at 59.997 kb on + strand at 59.997 kb on + strand at 60.005 kb on - strand at 60.005 kb on - strand at 60.005 kb on - strand at 60.010 kb on - strand at 60.039 kb on - strand at 60.039 kb on - strand at 60.116 kb on + strand, within SO0054 at 60.123 kb on - strand, within SO0054 at 60.124 kb on - strand, within SO0054 at 60.124 kb on - strand, within SO0054 at 60.223 kb on + strand, within SO0054 at 60.231 kb on - strand, within SO0054 at 60.231 kb on - strand, within SO0054 at 60.252 kb on - strand, within SO0054 at 60.281 kb on - strand, within SO0054 at 60.325 kb on + strand, within SO0054 at 60.325 kb on + strand, within SO0054 at 60.325 kb on + strand, within SO0054 at 60.325 kb on + strand, within SO0054 at 60.325 kb on + strand, within SO0054 at 60.325 kb on + strand, within SO0054 at 60.333 kb on - strand, within SO0054 at 60.333 kb on - strand, within SO0054 at 60.333 kb on - strand, within SO0054 at 60.333 kb on - strand, within SO0054 at 60.333 kb on - strand, within SO0054 at 60.376 kb on + strand, within SO0054 at 60.381 kb on + strand, within SO0054 at 60.384 kb on - strand, within SO0054 at 60.467 kb on + strand, within SO0054 at 60.475 kb on + strand, within SO0054 at 60.475 kb on - strand, within SO0054 at 60.499 kb on + strand, within SO0054 at 60.534 kb on - strand, within SO0054 at 60.596 kb on - strand, within SO0054
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 57,695 + SO0050 0.66 -1.4 57,728 + SO0050 0.73 -3.9 57,811 + -1.7 57,893 + SO0051 0.72 -2.8 57,893 + SO0051 0.72 -3.7 57,979 + -3.3 57,979 + -1.4 57,989 + -0.6 58,027 + -1.1 58,027 + -2.2 58,027 + -2.0 58,027 + -1.4 59,635 + -0.4 59,709 + +0.3 59,709 + +0.5 59,709 + -0.7 59,796 + -0.0 59,796 + -0.0 59,804 - +0.6 59,804 - +0.2 59,804 - -0.4 59,883 - +1.6 59,945 + +0.2 59,945 + +0.2 59,953 - -1.3 59,961 - -0.4 59,961 - +0.6 59,997 + -0.3 59,997 + +1.2 60,005 - -1.4 60,005 - +0.4 60,005 - +0.1 60,010 - -0.2 60,039 - -0.3 60,039 - +0.1 60,116 + SO0054 0.14 +0.2 60,123 - SO0054 0.15 +0.1 60,124 - SO0054 0.15 -0.2 60,124 - SO0054 0.15 -0.7 60,223 + SO0054 0.23 -0.3 60,231 - SO0054 0.24 +0.3 60,231 - SO0054 0.24 -0.4 60,252 - SO0054 0.25 +1.0 60,281 - SO0054 0.28 -0.3 60,325 + SO0054 0.32 -0.4 60,325 + SO0054 0.32 -0.2 60,325 + SO0054 0.32 -1.7 60,325 + SO0054 0.32 +0.3 60,325 + SO0054 0.32 -0.5 60,325 + SO0054 0.32 +0.8 60,333 - SO0054 0.32 +1.4 60,333 - SO0054 0.32 -0.3 60,333 - SO0054 0.32 -1.3 60,333 - SO0054 0.32 +0.1 60,333 - SO0054 0.32 +0.8 60,376 + SO0054 0.36 -0.3 60,381 + SO0054 0.36 +0.0 60,384 - SO0054 0.37 -0.2 60,467 + SO0054 0.43 -1.6 60,475 + SO0054 0.44 +0.5 60,475 - SO0054 0.44 +0.0 60,499 + SO0054 0.46 -0.6 60,534 - SO0054 0.49 -0.2 60,596 - SO0054 0.54 -0.3
Or see this region's nucleotide sequence