Experiment: Gentamicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trkA and sun are separated by 15 nucleotides sun and fmt are separated by 2 nucleotides fmt and def-1 are separated by 15 nucleotides
SO0029: trkA - potassium uptake protein TrkA (NCBI ptt file), at 33,706 to 35,115
trkA
SO0030: sun - sun protein (NCBI ptt file), at 35,131 to 36,417
sun
SO0031: fmt - methionyl-tRNA formyltransferase (NCBI ptt file), at 36,420 to 37,376
fmt
SO0032: def-1 - polypeptide deformylase (NCBI ptt file), at 37,392 to 37,898
def-1
Position (kb) 35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 34.621 kb on + strand, within trkA at 35.212 kb on - strand at 35.242 kb on - strand at 35.242 kb on - strand at 35.243 kb on + strand at 35.286 kb on - strand, within sun at 35.301 kb on + strand, within sun at 35.663 kb on - strand, within sun at 35.730 kb on + strand, within sun at 35.740 kb on + strand, within sun at 35.764 kb on - strand, within sun at 35.843 kb on + strand, within sun at 35.843 kb on + strand, within sun at 35.915 kb on - strand, within sun at 35.922 kb on + strand, within sun at 35.937 kb on + strand, within sun at 35.937 kb on + strand, within sun at 36.046 kb on + strand, within sun at 36.068 kb on + strand, within sun at 36.120 kb on - strand, within sun at 36.142 kb on + strand, within sun at 36.190 kb on - strand, within sun at 36.227 kb on + strand, within sun at 36.276 kb on + strand, within sun at 36.286 kb on + strand, within sun at 36.286 kb on - strand, within sun at 36.303 kb on - strand at 36.359 kb on - strand at 36.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.005 mg/ml remove 34,621 + trkA SO0029 0.65 -2.0 35,212 - -1.2 35,242 - -0.6 35,242 - -1.5 35,243 + -3.5 35,286 - sun SO0030 0.12 -1.4 35,301 + sun SO0030 0.13 -1.6 35,663 - sun SO0030 0.41 -0.1 35,730 + sun SO0030 0.47 -0.5 35,740 + sun SO0030 0.47 -2.9 35,764 - sun SO0030 0.49 -0.8 35,843 + sun SO0030 0.55 -2.3 35,843 + sun SO0030 0.55 -1.3 35,915 - sun SO0030 0.61 -1.0 35,922 + sun SO0030 0.61 -0.3 35,937 + sun SO0030 0.63 -1.7 35,937 + sun SO0030 0.63 -0.9 36,046 + sun SO0030 0.71 -1.4 36,068 + sun SO0030 0.73 -1.1 36,120 - sun SO0030 0.77 -0.4 36,142 + sun SO0030 0.79 -0.9 36,190 - sun SO0030 0.82 -1.0 36,227 + sun SO0030 0.85 +0.4 36,276 + sun SO0030 0.89 -0.9 36,286 + sun SO0030 0.90 -1.2 36,286 - sun SO0030 0.90 -0.9 36,303 - -0.6 36,359 - -1.3 36,460 - -1.1
Or see this region's nucleotide sequence