Experiment: m.b. Cobalt chloride 0.32 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2442 and HP15_2443 are separated by 100 nucleotides HP15_2443 and HP15_2444 are separated by 87 nucleotides HP15_2444 and HP15_2445 are separated by 22 nucleotides HP15_2445 and HP15_2446 are separated by 7 nucleotides HP15_2446 and HP15_2447 are separated by 94 nucleotides
HP15_2442: HP15_2442 - RNA polymerase, sigma 54 subunit, RpoN, at 2,579,904 to 2,581,430
_2442
HP15_2443: HP15_2443 - sigma 54 modulation protein/ribosomal protein S30EA, at 2,581,531 to 2,581,839
_2443
HP15_2444: HP15_2444 - PTS IIA-like nitrogen-regulatory protein PtsN, at 2,581,927 to 2,582,397
_2444
HP15_2445: HP15_2445 - protein yhbJ, at 2,582,420 to 2,583,310
_2445
HP15_2446: HP15_2446 - phosphotransferase system, phosphocarrier protein Hpr, at 2,583,318 to 2,583,587
_2446
HP15_2447: HP15_2447 - magnesium transporter, at 2,583,682 to 2,585,043
_2447
Position (kb)
2582
2583
2584 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2581.452 kb on - strand at 2581.556 kb on + strand at 2581.710 kb on + strand, within HP15_2443 at 2582.528 kb on + strand, within HP15_2445 at 2582.574 kb on - strand, within HP15_2445 at 2582.858 kb on - strand, within HP15_2445 at 2582.893 kb on - strand, within HP15_2445 at 2583.009 kb on - strand, within HP15_2445 at 2583.141 kb on - strand, within HP15_2445 at 2583.199 kb on - strand, within HP15_2445 at 2583.206 kb on + strand, within HP15_2445 at 2583.255 kb on - strand at 2583.886 kb on + strand, within HP15_2447
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.32 mM remove 2,581,452 - +4.7 2,581,556 + +1.5 2,581,710 + HP15_2443 0.58 +0.5 2,582,528 + HP15_2445 0.12 -0.2 2,582,574 - HP15_2445 0.17 +0.0 2,582,858 - HP15_2445 0.49 +0.3 2,582,893 - HP15_2445 0.53 -0.3 2,583,009 - HP15_2445 0.66 +1.3 2,583,141 - HP15_2445 0.81 +1.1 2,583,199 - HP15_2445 0.87 +1.3 2,583,206 + HP15_2445 0.88 +0.1 2,583,255 - +0.1 2,583,886 + HP15_2447 0.15 -2.2
Or see this region's nucleotide sequence