Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dfp and dut are separated by 0 nucleotides dut and SO4251 are separated by 31 nucleotides SO4251 and SO4252 are separated by 134 nucleotides
SO4249: dfp - DNA/pantothenate metabolism flavoprotein (NCBI ptt file), at 4,426,076 to 4,427,302
dfp
SO4250: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,427,303 to 4,427,761
dut
SO4251: SO4251 - transcriptional regulator, TetR family (NCBI ptt file), at 4,427,793 to 4,428,386
SO4251
SO4252: SO4252 - prolyl oligopeptidase family protein (NCBI ptt file), at 4,428,521 to 4,430,509
SO4252
Position (kb)
4427
4428
4429 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4427.375 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.514 kb on + strand, within dut at 4427.519 kb on + strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.522 kb on - strand, within dut at 4427.546 kb on - strand, within dut at 4427.622 kb on + strand, within dut at 4427.665 kb on - strand, within dut at 4427.791 kb on - strand at 4427.795 kb on + strand at 4427.840 kb on - strand at 4427.840 kb on - strand at 4427.863 kb on + strand, within SO4251 at 4427.908 kb on + strand, within SO4251 at 4427.993 kb on + strand, within SO4251 at 4428.036 kb on + strand, within SO4251 at 4428.057 kb on + strand, within SO4251 at 4428.232 kb on - strand, within SO4251 at 4428.252 kb on + strand, within SO4251 at 4428.338 kb on - strand at 4428.655 kb on - strand at 4428.777 kb on + strand, within SO4252 at 4428.795 kb on + strand, within SO4252 at 4428.803 kb on - strand, within SO4252 at 4428.805 kb on + strand, within SO4252 at 4428.837 kb on - strand, within SO4252 at 4428.871 kb on - strand, within SO4252 at 4428.985 kb on + strand, within SO4252 at 4429.036 kb on + strand, within SO4252 at 4429.036 kb on + strand, within SO4252 at 4429.039 kb on - strand, within SO4252 at 4429.044 kb on - strand, within SO4252 at 4429.044 kb on - strand, within SO4252 at 4429.104 kb on - strand, within SO4252 at 4429.151 kb on + strand, within SO4252 at 4429.189 kb on - strand, within SO4252 at 4429.200 kb on - strand, within SO4252 at 4429.229 kb on + strand, within SO4252 at 4429.255 kb on + strand, within SO4252 at 4429.313 kb on - strand, within SO4252 at 4429.342 kb on - strand, within SO4252
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 4,427,375 + dut SO4250 0.16 -2.7 4,427,514 + dut SO4250 0.46 -1.0 4,427,514 + dut SO4250 0.46 -0.8 4,427,514 + dut SO4250 0.46 -0.2 4,427,514 + dut SO4250 0.46 -2.9 4,427,514 + dut SO4250 0.46 -2.4 4,427,519 + dut SO4250 0.47 -2.3 4,427,522 - dut SO4250 0.48 -1.3 4,427,522 - dut SO4250 0.48 -1.4 4,427,522 - dut SO4250 0.48 -1.8 4,427,546 - dut SO4250 0.53 -2.4 4,427,622 + dut SO4250 0.69 -0.5 4,427,665 - dut SO4250 0.79 -2.9 4,427,791 - -3.2 4,427,795 + -0.0 4,427,840 - -2.9 4,427,840 - +1.4 4,427,863 + SO4251 0.12 -1.4 4,427,908 + SO4251 0.19 -1.7 4,427,993 + SO4251 0.34 -3.5 4,428,036 + SO4251 0.41 -1.8 4,428,057 + SO4251 0.44 -1.5 4,428,232 - SO4251 0.74 -1.6 4,428,252 + SO4251 0.77 -0.6 4,428,338 - -0.7 4,428,655 - +1.9 4,428,777 + SO4252 0.13 +0.3 4,428,795 + SO4252 0.14 -0.4 4,428,803 - SO4252 0.14 -2.8 4,428,805 + SO4252 0.14 -0.8 4,428,837 - SO4252 0.16 -0.3 4,428,871 - SO4252 0.18 +0.6 4,428,985 + SO4252 0.23 +1.3 4,429,036 + SO4252 0.26 +1.9 4,429,036 + SO4252 0.26 +0.3 4,429,039 - SO4252 0.26 +0.8 4,429,044 - SO4252 0.26 -0.8 4,429,044 - SO4252 0.26 -0.7 4,429,104 - SO4252 0.29 -1.9 4,429,151 + SO4252 0.32 -1.2 4,429,189 - SO4252 0.34 -0.3 4,429,200 - SO4252 0.34 +0.8 4,429,229 + SO4252 0.36 +0.3 4,429,255 + SO4252 0.37 +3.7 4,429,313 - SO4252 0.40 +0.2 4,429,342 - SO4252 0.41 -2.3
Or see this region's nucleotide sequence