Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3974 and SO3975 are separated by 22 nucleotides SO3975 and SO3976 are separated by 534 nucleotides
SO3974: SO3974 - conserved domain protein (NCBI ptt file), at 4,111,729 to 4,112,277
SO3974
SO3975: SO3975 - conserved hypothetical protein (NCBI ptt file), at 4,112,300 to 4,113,094
SO3975
SO3976: SO3976 - GAF domain protein (NCBI ptt file), at 4,113,629 to 4,114,408
SO3976
Position (kb)
4112
4113
4114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4111.308 kb on + strand at 4111.364 kb on + strand at 4111.378 kb on + strand at 4111.407 kb on + strand at 4111.407 kb on + strand at 4111.567 kb on - strand at 4111.567 kb on - strand at 4111.623 kb on + strand at 4111.623 kb on + strand at 4111.623 kb on + strand at 4111.631 kb on - strand at 4111.631 kb on - strand at 4111.631 kb on - strand at 4111.631 kb on - strand at 4111.631 kb on - strand at 4111.631 kb on - strand at 4111.631 kb on - strand at 4111.680 kb on + strand at 4111.680 kb on + strand at 4111.680 kb on + strand at 4111.687 kb on + strand at 4111.687 kb on + strand at 4111.688 kb on - strand at 4111.688 kb on - strand at 4111.688 kb on - strand at 4111.688 kb on - strand at 4111.688 kb on - strand at 4111.688 kb on - strand at 4111.688 kb on - strand at 4111.699 kb on + strand at 4111.763 kb on + strand at 4111.769 kb on + strand at 4111.771 kb on - strand at 4111.826 kb on + strand, within SO3974 at 4111.826 kb on + strand, within SO3974 at 4111.826 kb on - strand, within SO3974 at 4111.833 kb on - strand, within SO3974 at 4111.839 kb on - strand, within SO3974 at 4111.861 kb on - strand, within SO3974 at 4111.863 kb on - strand, within SO3974 at 4111.876 kb on - strand, within SO3974 at 4111.887 kb on + strand, within SO3974 at 4111.911 kb on + strand, within SO3974 at 4111.966 kb on - strand, within SO3974 at 4111.983 kb on + strand, within SO3974 at 4111.998 kb on - strand, within SO3974 at 4111.999 kb on + strand, within SO3974 at 4112.009 kb on + strand, within SO3974 at 4112.046 kb on + strand, within SO3974 at 4112.089 kb on + strand, within SO3974 at 4112.089 kb on + strand, within SO3974 at 4112.113 kb on - strand, within SO3974 at 4112.286 kb on + strand at 4112.288 kb on + strand at 4112.306 kb on - strand at 4112.377 kb on + strand at 4112.488 kb on - strand, within SO3975 at 4112.488 kb on - strand, within SO3975 at 4112.593 kb on - strand, within SO3975 at 4112.866 kb on + strand, within SO3975 at 4113.014 kb on + strand, within SO3975 at 4113.288 kb on + strand at 4113.288 kb on + strand at 4113.288 kb on + strand at 4113.288 kb on + strand at 4113.296 kb on - strand at 4113.296 kb on - strand at 4113.296 kb on - strand at 4113.704 kb on + strand at 4113.704 kb on + strand at 4113.808 kb on + strand, within SO3976 at 4113.816 kb on + strand, within SO3976 at 4113.826 kb on + strand, within SO3976 at 4113.904 kb on + strand, within SO3976 at 4113.918 kb on + strand, within SO3976 at 4113.918 kb on + strand, within SO3976 at 4113.918 kb on + strand, within SO3976 at 4113.934 kb on + strand, within SO3976 at 4113.937 kb on - strand, within SO3976 at 4113.947 kb on - strand, within SO3976 at 4113.947 kb on - strand, within SO3976 at 4114.067 kb on - strand, within SO3976
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 4,111,308 + -0.6 4,111,364 + -2.0 4,111,378 + -1.8 4,111,407 + -0.8 4,111,407 + -0.5 4,111,567 - +1.8 4,111,567 - +0.4 4,111,623 + +0.3 4,111,623 + -0.6 4,111,623 + -1.4 4,111,631 - -2.1 4,111,631 - -0.6 4,111,631 - +2.8 4,111,631 - +0.5 4,111,631 - +0.5 4,111,631 - -0.4 4,111,631 - +0.5 4,111,680 + -1.8 4,111,680 + +1.2 4,111,680 + +1.6 4,111,687 + +0.2 4,111,687 + +0.7 4,111,688 - -1.3 4,111,688 - -0.5 4,111,688 - -0.3 4,111,688 - +0.1 4,111,688 - +0.2 4,111,688 - +0.9 4,111,688 - +0.8 4,111,699 + +0.7 4,111,763 + -0.2 4,111,769 + -1.2 4,111,771 - -2.6 4,111,826 + SO3974 0.18 -2.7 4,111,826 + SO3974 0.18 -0.2 4,111,826 - SO3974 0.18 +2.4 4,111,833 - SO3974 0.19 +1.3 4,111,839 - SO3974 0.20 +0.7 4,111,861 - SO3974 0.24 +1.3 4,111,863 - SO3974 0.24 +0.6 4,111,876 - SO3974 0.27 -2.2 4,111,887 + SO3974 0.29 +0.3 4,111,911 + SO3974 0.33 +1.0 4,111,966 - SO3974 0.43 -0.6 4,111,983 + SO3974 0.46 +0.1 4,111,998 - SO3974 0.49 -3.2 4,111,999 + SO3974 0.49 -2.2 4,112,009 + SO3974 0.51 -1.2 4,112,046 + SO3974 0.58 -0.0 4,112,089 + SO3974 0.66 -2.6 4,112,089 + SO3974 0.66 +0.1 4,112,113 - SO3974 0.70 -1.8 4,112,286 + +1.4 4,112,288 + -0.5 4,112,306 - +1.3 4,112,377 + +0.4 4,112,488 - SO3975 0.24 +0.6 4,112,488 - SO3975 0.24 +2.0 4,112,593 - SO3975 0.37 +1.5 4,112,866 + SO3975 0.71 -1.0 4,113,014 + SO3975 0.90 +0.7 4,113,288 + -0.8 4,113,288 + +1.0 4,113,288 + +0.7 4,113,288 + -2.1 4,113,296 - +0.6 4,113,296 - +1.8 4,113,296 - -1.0 4,113,704 + +0.4 4,113,704 + +0.4 4,113,808 + SO3976 0.23 +1.6 4,113,816 + SO3976 0.24 +0.7 4,113,826 + SO3976 0.25 -1.4 4,113,904 + SO3976 0.35 -1.0 4,113,918 + SO3976 0.37 -0.3 4,113,918 + SO3976 0.37 -2.2 4,113,918 + SO3976 0.37 +1.6 4,113,934 + SO3976 0.39 +0.1 4,113,937 - SO3976 0.39 -0.2 4,113,947 - SO3976 0.41 +0.6 4,113,947 - SO3976 0.41 +0.4 4,114,067 - SO3976 0.56 -1.9
Or see this region's nucleotide sequence