Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3253 and flgM are separated by 75 nucleotides flgM and flgN are separated by 9 nucleotides flgN and SO3256 are separated by 117 nucleotides SO3256 and SO3257 overlap by 4 nucleotides SO3257 and SO3258 are separated by 273 nucleotides
SO3253: SO3253 - flagellar basal-body P-ring formation protein FlgA, putative (NCBI ptt file), at 3,390,904 to 3,391,611
SO3253
SO3254: flgM - negative regulator of flagellin synthesis FlgM (NCBI ptt file), at 3,391,687 to 3,392,007
flgM
SO3255: flgN - flagellar biosynthetic protein FlgN (NCBI ptt file), at 3,392,017 to 3,392,442
flgN
SO3256: SO3256 - conserved hypothetical protein (NCBI ptt file), at 3,392,560 to 3,393,009
SO3256
SO3257: SO3257 - conserved hypothetical protein (NCBI ptt file), at 3,393,006 to 3,393,638
SO3257
SO3258: SO3258 - conserved hypothetical protein (NCBI ptt file), at 3,393,912 to 3,395,087
SO3258
Position (kb)
3392
3393
3394 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3391.616 kb on + strand at 3391.667 kb on + strand at 3391.731 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.745 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.845 kb on + strand, within flgM at 3391.987 kb on + strand at 3392.042 kb on + strand at 3392.253 kb on + strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.266 kb on - strand, within flgN at 3392.336 kb on + strand, within flgN at 3392.341 kb on + strand, within flgN at 3392.349 kb on - strand, within flgN at 3392.394 kb on + strand, within flgN at 3392.529 kb on - strand at 3392.530 kb on + strand at 3392.540 kb on + strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.548 kb on - strand at 3392.685 kb on + strand, within SO3256 at 3392.742 kb on + strand, within SO3256 at 3392.891 kb on - strand, within SO3256 at 3392.904 kb on + strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3392.912 kb on - strand, within SO3256 at 3393.089 kb on + strand, within SO3257 at 3393.101 kb on + strand, within SO3257 at 3393.160 kb on - strand, within SO3257 at 3393.331 kb on - strand, within SO3257 at 3393.636 kb on - strand at 3393.640 kb on + strand at 3393.648 kb on - strand at 3393.677 kb on - strand at 3393.717 kb on - strand at 3393.758 kb on - strand at 3393.788 kb on - strand at 3393.843 kb on + strand at 3393.861 kb on - strand at 3393.872 kb on - strand at 3393.949 kb on + strand at 3393.957 kb on - strand at 3393.959 kb on + strand at 3393.971 kb on + strand at 3393.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 3,391,616 + +0.9 3,391,667 + +0.2 3,391,731 + flgM SO3254 0.14 -1.2 3,391,745 + flgM SO3254 0.18 -2.4 3,391,745 + flgM SO3254 0.18 +0.5 3,391,745 + flgM SO3254 0.18 -0.4 3,391,745 + flgM SO3254 0.18 +0.2 3,391,745 + flgM SO3254 0.18 +0.5 3,391,745 + flgM SO3254 0.18 -0.9 3,391,745 + flgM SO3254 0.18 +0.4 3,391,745 + flgM SO3254 0.18 -2.7 3,391,745 + flgM SO3254 0.18 +0.9 3,391,745 + flgM SO3254 0.18 -0.5 3,391,845 + flgM SO3254 0.49 -0.3 3,391,845 + flgM SO3254 0.49 -0.7 3,391,987 + -0.8 3,392,042 + -2.0 3,392,253 + flgN SO3255 0.55 -1.3 3,392,266 - flgN SO3255 0.58 -0.1 3,392,266 - flgN SO3255 0.58 +1.6 3,392,336 + flgN SO3255 0.75 -0.3 3,392,341 + flgN SO3255 0.76 +1.9 3,392,349 - flgN SO3255 0.78 -0.8 3,392,394 + flgN SO3255 0.88 +0.8 3,392,529 - +0.9 3,392,530 + -0.4 3,392,540 + -1.4 3,392,548 - +0.4 3,392,548 - -2.0 3,392,548 - -1.9 3,392,685 + SO3256 0.28 -0.2 3,392,742 + SO3256 0.40 +1.0 3,392,891 - SO3256 0.74 -0.9 3,392,904 + SO3256 0.76 -0.0 3,392,912 - SO3256 0.78 -0.1 3,392,912 - SO3256 0.78 +0.3 3,392,912 - SO3256 0.78 +0.3 3,393,089 + SO3257 0.13 +0.8 3,393,101 + SO3257 0.15 -1.4 3,393,160 - SO3257 0.24 +0.2 3,393,331 - SO3257 0.51 +1.1 3,393,636 - +0.7 3,393,640 + +0.3 3,393,648 - +0.1 3,393,677 - +1.0 3,393,717 - +0.4 3,393,758 - -0.0 3,393,788 - -1.9 3,393,843 + -2.8 3,393,861 - -1.4 3,393,872 - +0.3 3,393,949 + +1.9 3,393,957 - +0.8 3,393,959 + +1.1 3,393,971 + -1.9 3,393,979 - +1.1
Or see this region's nucleotide sequence