Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadD-1 and SO2582 are separated by 197 nucleotides SO2582 and SO2583 are separated by 21 nucleotides SO2583 and SO2584 are separated by 144 nucleotides
SO2581: fadD-1 - long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 2,707,886 to 2,709,559
fadD-1
SO2582: SO2582 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,709,757 to 2,710,623
SO2582
SO2583: SO2583 - hypothetical protein (NCBI ptt file), at 2,710,645 to 2,711,457
SO2583
SO2584: SO2584 - hypothetical protein (NCBI ptt file), at 2,711,602 to 2,712,315
SO2584
Position (kb)
2709
2710
2711 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2708.824 kb on + strand, within fadD-1 at 2708.868 kb on + strand, within fadD-1 at 2708.876 kb on - strand, within fadD-1 at 2709.065 kb on + strand, within fadD-1 at 2709.151 kb on + strand, within fadD-1 at 2709.249 kb on + strand, within fadD-1 at 2709.250 kb on + strand, within fadD-1 at 2709.294 kb on + strand, within fadD-1 at 2709.302 kb on - strand, within fadD-1 at 2709.339 kb on + strand, within fadD-1 at 2709.431 kb on - strand at 2709.461 kb on - strand at 2709.497 kb on - strand at 2709.606 kb on - strand at 2709.656 kb on - strand at 2709.866 kb on + strand, within SO2582 at 2709.874 kb on - strand, within SO2582 at 2709.874 kb on - strand, within SO2582 at 2709.874 kb on - strand, within SO2582 at 2709.960 kb on + strand, within SO2582 at 2709.967 kb on + strand, within SO2582 at 2709.968 kb on - strand, within SO2582 at 2709.968 kb on - strand, within SO2582 at 2710.107 kb on - strand, within SO2582 at 2710.138 kb on - strand, within SO2582 at 2710.138 kb on - strand, within SO2582 at 2710.214 kb on + strand, within SO2582 at 2710.236 kb on - strand, within SO2582 at 2710.723 kb on + strand at 2710.763 kb on + strand, within SO2583 at 2710.765 kb on - strand, within SO2583 at 2710.765 kb on - strand, within SO2583 at 2710.771 kb on - strand, within SO2583 at 2710.900 kb on + strand, within SO2583 at 2710.938 kb on + strand, within SO2583 at 2710.938 kb on + strand, within SO2583 at 2711.165 kb on + strand, within SO2583 at 2711.173 kb on - strand, within SO2583 at 2711.205 kb on - strand, within SO2583 at 2711.324 kb on - strand, within SO2583 at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.398 kb on + strand at 2711.406 kb on - strand at 2711.406 kb on - strand at 2711.481 kb on + strand at 2711.483 kb on - strand at 2711.536 kb on + strand at 2711.593 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 2,708,824 + fadD-1 SO2581 0.56 -2.8 2,708,868 + fadD-1 SO2581 0.59 -1.8 2,708,876 - fadD-1 SO2581 0.59 +0.5 2,709,065 + fadD-1 SO2581 0.70 -0.1 2,709,151 + fadD-1 SO2581 0.76 -0.3 2,709,249 + fadD-1 SO2581 0.81 -0.3 2,709,250 + fadD-1 SO2581 0.81 -0.1 2,709,294 + fadD-1 SO2581 0.84 -0.8 2,709,302 - fadD-1 SO2581 0.85 +1.5 2,709,339 + fadD-1 SO2581 0.87 -2.0 2,709,431 - -0.1 2,709,461 - -0.1 2,709,497 - -2.0 2,709,606 - -2.3 2,709,656 - -0.2 2,709,866 + SO2582 0.13 -2.3 2,709,874 - SO2582 0.13 +0.2 2,709,874 - SO2582 0.13 -2.8 2,709,874 - SO2582 0.13 -0.2 2,709,960 + SO2582 0.23 -2.9 2,709,967 + SO2582 0.24 -0.6 2,709,968 - SO2582 0.24 -1.1 2,709,968 - SO2582 0.24 +0.2 2,710,107 - SO2582 0.40 +0.1 2,710,138 - SO2582 0.44 +0.4 2,710,138 - SO2582 0.44 -1.7 2,710,214 + SO2582 0.53 +0.7 2,710,236 - SO2582 0.55 -1.6 2,710,723 + +0.8 2,710,763 + SO2583 0.15 +0.3 2,710,765 - SO2583 0.15 -0.3 2,710,765 - SO2583 0.15 -0.4 2,710,771 - SO2583 0.15 +0.6 2,710,900 + SO2583 0.31 -0.7 2,710,938 + SO2583 0.36 +1.3 2,710,938 + SO2583 0.36 -1.9 2,711,165 + SO2583 0.64 -1.7 2,711,173 - SO2583 0.65 +1.4 2,711,205 - SO2583 0.69 +0.4 2,711,324 - SO2583 0.84 +0.5 2,711,398 + -2.4 2,711,398 + +0.1 2,711,398 + +0.9 2,711,398 + -1.6 2,711,398 + +0.7 2,711,398 + -0.5 2,711,398 + +0.8 2,711,398 + +3.0 2,711,406 - -2.5 2,711,406 - +0.9 2,711,481 + -0.7 2,711,483 - +0.6 2,711,536 + -2.6 2,711,593 + -3.5
Or see this region's nucleotide sequence