Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gapA-2 and SO2346 are separated by 224 nucleotides SO2346 and gapA-3 are separated by 205 nucleotides
SO2345: gapA-2 - glyceraldehyde 3-phosphate dehydrogenase (NCBI ptt file), at 2,452,794 to 2,453,804
gapA-2
SO2346: SO2346 - conserved hypothetical protein (NCBI ptt file), at 2,454,029 to 2,454,907
SO2346
SO2347: gapA-3 - glyceraldehyde 3-phosphate dehydrogenase (NCBI ptt file), at 2,455,113 to 2,456,546
gapA-3
Position (kb)
2454
2455 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2454.065 kb on + strand at 2454.073 kb on - strand at 2454.081 kb on - strand at 2454.096 kb on - strand at 2454.096 kb on - strand at 2454.111 kb on - strand at 2454.289 kb on - strand, within SO2346 at 2454.300 kb on + strand, within SO2346 at 2454.300 kb on + strand, within SO2346 at 2454.308 kb on - strand, within SO2346 at 2454.309 kb on + strand, within SO2346 at 2454.361 kb on + strand, within SO2346 at 2454.369 kb on - strand, within SO2346 at 2454.369 kb on - strand, within SO2346 at 2454.411 kb on - strand, within SO2346 at 2454.591 kb on + strand, within SO2346 at 2454.606 kb on + strand, within SO2346 at 2454.639 kb on + strand, within SO2346 at 2454.639 kb on + strand, within SO2346 at 2454.647 kb on - strand, within SO2346 at 2454.652 kb on - strand, within SO2346 at 2454.657 kb on - strand, within SO2346 at 2454.680 kb on + strand, within SO2346 at 2454.690 kb on + strand, within SO2346 at 2454.719 kb on - strand, within SO2346 at 2454.765 kb on + strand, within SO2346 at 2454.765 kb on + strand, within SO2346 at 2455.062 kb on + strand at 2455.107 kb on + strand at 2455.190 kb on + strand at 2455.340 kb on - strand, within gapA-3 at 2455.354 kb on + strand, within gapA-3 at 2455.362 kb on - strand, within gapA-3 at 2455.400 kb on - strand, within gapA-3 at 2455.833 kb on + strand, within gapA-3 at 2455.839 kb on + strand, within gapA-3
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 2,454,065 + +1.3 2,454,073 - -0.4 2,454,081 - +0.5 2,454,096 - +0.2 2,454,096 - +0.8 2,454,111 - +1.1 2,454,289 - SO2346 0.30 -2.2 2,454,300 + SO2346 0.31 -0.6 2,454,300 + SO2346 0.31 +0.3 2,454,308 - SO2346 0.32 -1.2 2,454,309 + SO2346 0.32 +0.9 2,454,361 + SO2346 0.38 +0.9 2,454,369 - SO2346 0.39 -0.2 2,454,369 - SO2346 0.39 +0.6 2,454,411 - SO2346 0.43 +0.9 2,454,591 + SO2346 0.64 +0.3 2,454,606 + SO2346 0.66 +2.8 2,454,639 + SO2346 0.69 -0.8 2,454,639 + SO2346 0.69 -0.5 2,454,647 - SO2346 0.70 +2.3 2,454,652 - SO2346 0.71 -0.1 2,454,657 - SO2346 0.71 +0.6 2,454,680 + SO2346 0.74 +0.0 2,454,690 + SO2346 0.75 +0.8 2,454,719 - SO2346 0.78 +1.2 2,454,765 + SO2346 0.84 +0.8 2,454,765 + SO2346 0.84 -1.3 2,455,062 + +1.5 2,455,107 + +3.2 2,455,190 + -0.5 2,455,340 - gapA-3 SO2347 0.16 +0.4 2,455,354 + gapA-3 SO2347 0.17 -0.0 2,455,362 - gapA-3 SO2347 0.17 -1.0 2,455,400 - gapA-3 SO2347 0.20 +0.5 2,455,833 + gapA-3 SO2347 0.50 -1.0 2,455,839 + gapA-3 SO2347 0.51 -1.6
Or see this region's nucleotide sequence