Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1969 and SO1970 are separated by 198 nucleotides SO1970 and SO1971 are separated by 127 nucleotides SO1971 and SO1973 are separated by 570 nucleotides
SO1969: SO1969 - conserved hypothetical protein (NCBI ptt file), at 2,074,125 to 2,074,586
SO1969
SO1970: SO1970 - hypothetical protein (NCBI ptt file), at 2,074,785 to 2,075,264
SO1970
SO1971: SO1971 - AMP-binding family protein (NCBI ptt file), at 2,075,392 to 2,077,128
SO1971
SO1973: SO1973 - hypothetical ABC transporter protein (NCBI ptt file), at 2,077,699 to 2,078,406
SO1973
Position (kb)
2075
2076
2077
2078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2074.504 kb on - strand, within SO1969 at 2074.655 kb on + strand at 2074.666 kb on + strand at 2074.680 kb on + strand at 2074.688 kb on - strand at 2074.688 kb on - strand at 2074.688 kb on - strand at 2074.688 kb on - strand at 2074.714 kb on - strand at 2074.724 kb on - strand at 2074.724 kb on - strand at 2074.767 kb on - strand at 2074.767 kb on - strand at 2074.899 kb on - strand, within SO1970 at 2074.948 kb on + strand, within SO1970 at 2075.009 kb on + strand, within SO1970 at 2075.009 kb on + strand, within SO1970 at 2075.017 kb on - strand, within SO1970 at 2075.095 kb on + strand, within SO1970 at 2075.098 kb on - strand, within SO1970 at 2075.103 kb on - strand, within SO1970 at 2075.114 kb on + strand, within SO1970 at 2075.134 kb on + strand, within SO1970 at 2075.157 kb on - strand, within SO1970 at 2075.204 kb on - strand, within SO1970 at 2075.260 kb on - strand at 2075.289 kb on + strand at 2075.310 kb on - strand at 2075.310 kb on - strand at 2075.358 kb on - strand at 2075.506 kb on + strand at 2075.514 kb on - strand at 2075.537 kb on + strand at 2075.537 kb on + strand at 2075.545 kb on + strand at 2075.696 kb on + strand, within SO1971 at 2075.741 kb on + strand, within SO1971 at 2075.788 kb on + strand, within SO1971 at 2075.803 kb on + strand, within SO1971 at 2075.806 kb on + strand, within SO1971 at 2076.003 kb on + strand, within SO1971 at 2076.301 kb on - strand, within SO1971 at 2076.301 kb on - strand, within SO1971 at 2076.328 kb on + strand, within SO1971 at 2076.337 kb on + strand, within SO1971 at 2076.345 kb on - strand, within SO1971 at 2076.442 kb on + strand, within SO1971 at 2076.452 kb on + strand, within SO1971 at 2076.452 kb on + strand, within SO1971 at 2076.460 kb on - strand, within SO1971 at 2076.557 kb on - strand, within SO1971 at 2076.629 kb on + strand, within SO1971 at 2076.671 kb on - strand, within SO1971 at 2076.678 kb on + strand, within SO1971 at 2076.861 kb on + strand, within SO1971 at 2076.892 kb on + strand, within SO1971 at 2076.892 kb on + strand, within SO1971 at 2076.896 kb on - strand, within SO1971 at 2076.900 kb on - strand, within SO1971 at 2076.902 kb on + strand, within SO1971 at 2076.910 kb on - strand, within SO1971 at 2076.944 kb on + strand, within SO1971 at 2076.944 kb on + strand, within SO1971 at 2076.944 kb on + strand, within SO1971 at 2076.981 kb on + strand at 2077.004 kb on + strand at 2077.004 kb on + strand at 2077.044 kb on + strand at 2077.097 kb on + strand at 2077.184 kb on + strand at 2077.184 kb on + strand at 2077.189 kb on + strand at 2077.189 kb on + strand at 2077.189 kb on + strand at 2077.192 kb on - strand at 2077.192 kb on - strand at 2077.192 kb on - strand at 2077.192 kb on - strand at 2077.202 kb on - strand at 2077.262 kb on + strand at 2077.268 kb on - strand at 2077.269 kb on - strand at 2077.270 kb on - strand at 2077.272 kb on + strand at 2077.277 kb on + strand at 2077.285 kb on - strand at 2077.308 kb on + strand at 2077.308 kb on + strand at 2077.308 kb on + strand at 2077.308 kb on + strand at 2077.308 kb on + strand at 2077.310 kb on - strand at 2077.316 kb on - strand at 2077.316 kb on - strand at 2077.316 kb on - strand at 2077.316 kb on - strand at 2077.316 kb on - strand at 2077.375 kb on + strand at 2077.375 kb on + strand at 2077.375 kb on + strand at 2077.383 kb on - strand at 2077.383 kb on - strand at 2077.383 kb on - strand at 2077.383 kb on - strand at 2077.456 kb on + strand at 2077.489 kb on - strand at 2077.489 kb on - strand at 2077.489 kb on - strand at 2077.494 kb on + strand at 2077.502 kb on + strand at 2077.502 kb on + strand at 2077.502 kb on + strand at 2077.510 kb on - strand at 2077.510 kb on - strand at 2077.510 kb on - strand at 2077.522 kb on + strand at 2077.522 kb on + strand at 2077.548 kb on - strand at 2077.580 kb on - strand at 2077.634 kb on + strand at 2077.645 kb on + strand at 2077.658 kb on + strand at 2077.666 kb on - strand at 2077.694 kb on + strand at 2077.694 kb on + strand at 2077.702 kb on - strand at 2077.751 kb on - strand at 2077.758 kb on + strand at 2077.758 kb on + strand at 2077.758 kb on + strand at 2077.766 kb on - strand at 2077.824 kb on - strand, within SO1973 at 2077.832 kb on + strand, within SO1973 at 2077.840 kb on - strand, within SO1973 at 2077.840 kb on - strand, within SO1973 at 2077.844 kb on + strand, within SO1973 at 2077.902 kb on - strand, within SO1973 at 2078.011 kb on - strand, within SO1973 at 2078.041 kb on - strand, within SO1973 at 2078.080 kb on + strand, within SO1973 at 2078.081 kb on - strand, within SO1973 at 2078.081 kb on - strand, within SO1973 at 2078.104 kb on - strand, within SO1973
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 2,074,504 - SO1969 0.82 -0.5 2,074,655 + -0.7 2,074,666 + +1.7 2,074,680 + +0.8 2,074,688 - -0.4 2,074,688 - -0.4 2,074,688 - +1.0 2,074,688 - -1.5 2,074,714 - +0.5 2,074,724 - +2.1 2,074,724 - +2.3 2,074,767 - -0.6 2,074,767 - -2.2 2,074,899 - SO1970 0.24 -0.9 2,074,948 + SO1970 0.34 +0.2 2,075,009 + SO1970 0.47 -0.3 2,075,009 + SO1970 0.47 +0.3 2,075,017 - SO1970 0.48 -0.5 2,075,095 + SO1970 0.65 -0.8 2,075,098 - SO1970 0.65 +1.8 2,075,103 - SO1970 0.66 +0.5 2,075,114 + SO1970 0.69 -0.2 2,075,134 + SO1970 0.73 -1.2 2,075,157 - SO1970 0.78 +1.1 2,075,204 - SO1970 0.87 -1.6 2,075,260 - -2.0 2,075,289 + -0.8 2,075,310 - +0.9 2,075,310 - -3.0 2,075,358 - +0.0 2,075,506 + -3.5 2,075,514 - +0.3 2,075,537 + +0.7 2,075,537 + +1.4 2,075,545 + +2.0 2,075,696 + SO1971 0.18 -1.1 2,075,741 + SO1971 0.20 -0.1 2,075,788 + SO1971 0.23 +0.3 2,075,803 + SO1971 0.24 +0.3 2,075,806 + SO1971 0.24 -0.5 2,076,003 + SO1971 0.35 +0.2 2,076,301 - SO1971 0.52 -1.5 2,076,301 - SO1971 0.52 -0.2 2,076,328 + SO1971 0.54 +1.6 2,076,337 + SO1971 0.54 -0.7 2,076,345 - SO1971 0.55 -2.0 2,076,442 + SO1971 0.60 -0.2 2,076,452 + SO1971 0.61 +0.7 2,076,452 + SO1971 0.61 -1.5 2,076,460 - SO1971 0.61 -0.3 2,076,557 - SO1971 0.67 -2.6 2,076,629 + SO1971 0.71 -2.4 2,076,671 - SO1971 0.74 -0.4 2,076,678 + SO1971 0.74 -0.1 2,076,861 + SO1971 0.85 +0.8 2,076,892 + SO1971 0.86 -0.1 2,076,892 + SO1971 0.86 -1.9 2,076,896 - SO1971 0.87 +1.7 2,076,900 - SO1971 0.87 -2.4 2,076,902 + SO1971 0.87 -0.3 2,076,910 - SO1971 0.87 -0.5 2,076,944 + SO1971 0.89 +0.0 2,076,944 + SO1971 0.89 -1.0 2,076,944 + SO1971 0.89 +0.5 2,076,981 + -0.3 2,077,004 + +1.5 2,077,004 + +1.2 2,077,044 + -0.2 2,077,097 + +1.0 2,077,184 + -1.7 2,077,184 + -0.0 2,077,189 + +1.8 2,077,189 + -0.8 2,077,189 + -2.1 2,077,192 - -0.4 2,077,192 - +0.9 2,077,192 - +1.6 2,077,192 - -0.7 2,077,202 - +0.8 2,077,262 + -0.5 2,077,268 - +0.1 2,077,269 - -0.5 2,077,270 - +0.4 2,077,272 + +0.6 2,077,277 + +0.0 2,077,285 - +1.5 2,077,308 + +0.4 2,077,308 + -0.1 2,077,308 + -0.4 2,077,308 + +1.1 2,077,308 + +2.0 2,077,310 - -0.0 2,077,316 - +0.8 2,077,316 - +1.9 2,077,316 - +1.1 2,077,316 - -1.5 2,077,316 - +0.5 2,077,375 + +0.9 2,077,375 + -1.3 2,077,375 + -1.1 2,077,383 - +0.9 2,077,383 - -0.6 2,077,383 - +0.2 2,077,383 - +0.7 2,077,456 + -0.1 2,077,489 - -2.3 2,077,489 - -1.9 2,077,489 - +0.7 2,077,494 + -0.1 2,077,502 + -0.7 2,077,502 + -0.0 2,077,502 + -0.7 2,077,510 - -1.4 2,077,510 - -0.7 2,077,510 - -1.7 2,077,522 + +0.5 2,077,522 + -1.0 2,077,548 - +1.0 2,077,580 - -0.2 2,077,634 + -0.3 2,077,645 + -1.8 2,077,658 + -2.3 2,077,666 - +2.4 2,077,694 + -0.9 2,077,694 + -0.5 2,077,702 - -0.5 2,077,751 - +1.1 2,077,758 + +0.2 2,077,758 + +1.0 2,077,758 + +0.9 2,077,766 - +0.4 2,077,824 - SO1973 0.18 -0.9 2,077,832 + SO1973 0.19 -2.6 2,077,840 - SO1973 0.20 +0.8 2,077,840 - SO1973 0.20 -0.3 2,077,844 + SO1973 0.20 +0.9 2,077,902 - SO1973 0.29 -1.2 2,078,011 - SO1973 0.44 +1.1 2,078,041 - SO1973 0.48 -0.5 2,078,080 + SO1973 0.54 +1.3 2,078,081 - SO1973 0.54 +0.2 2,078,081 - SO1973 0.54 -2.4 2,078,104 - SO1973 0.57 +0.9
Or see this region's nucleotide sequence