Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1654 and cysQ-2 are separated by 15 nucleotides cysQ-2 and SO1656 are separated by 62 nucleotides SO1656 and SO1657 are separated by 96 nucleotides SO1657 and SO1658 are separated by 4 nucleotides
SO1654: SO1654 - hypothetical protein (NCBI ptt file), at 1,742,372 to 1,742,725
SO1654
SO1655: cysQ-2 - cysQ protein (NCBI ptt file), at 1,742,741 to 1,743,502
cysQ-2
SO1656: SO1656 - ROK family protein (NCBI ptt file), at 1,743,565 to 1,744,485
SO1656
SO1657: SO1657 - conserved hypothetical protein (NCBI ptt file), at 1,744,582 to 1,745,316
SO1657
SO1658: SO1658 - hypothetical protein (NCBI ptt file), at 1,745,321 to 1,746,052
SO1658
Position (kb)
1743
1744
1745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1742.566 kb on + strand, within SO1654 at 1743.129 kb on - strand, within cysQ-2 at 1743.338 kb on - strand, within cysQ-2 at 1743.422 kb on + strand, within cysQ-2 at 1743.428 kb on + strand at 1743.430 kb on - strand at 1743.565 kb on - strand at 1743.640 kb on + strand at 1743.690 kb on - strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.776 kb on + strand, within SO1656 at 1743.784 kb on - strand, within SO1656 at 1743.784 kb on - strand, within SO1656 at 1743.784 kb on - strand, within SO1656 at 1743.818 kb on - strand, within SO1656 at 1743.820 kb on + strand, within SO1656 at 1743.836 kb on + strand, within SO1656 at 1743.855 kb on - strand, within SO1656 at 1743.879 kb on + strand, within SO1656 at 1743.976 kb on - strand, within SO1656 at 1744.020 kb on - strand, within SO1656 at 1744.073 kb on - strand, within SO1656 at 1744.313 kb on - strand, within SO1656 at 1744.327 kb on - strand, within SO1656 at 1744.349 kb on - strand, within SO1656 at 1744.463 kb on - strand at 1744.473 kb on - strand at 1744.557 kb on + strand at 1744.557 kb on + strand at 1744.623 kb on - strand at 1744.790 kb on - strand, within SO1657 at 1744.794 kb on + strand, within SO1657 at 1744.802 kb on - strand, within SO1657 at 1744.925 kb on - strand, within SO1657 at 1745.006 kb on - strand, within SO1657 at 1745.024 kb on - strand, within SO1657 at 1745.359 kb on + strand at 1745.378 kb on + strand at 1745.378 kb on + strand at 1745.378 kb on + strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.386 kb on - strand at 1745.464 kb on - strand, within SO1658 at 1745.480 kb on - strand, within SO1658
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 1,742,566 + SO1654 0.55 -1.0 1,742,566 + SO1654 0.55 +1.7 1,742,566 + SO1654 0.55 +0.2 1,742,566 + SO1654 0.55 -0.2 1,742,566 + SO1654 0.55 +0.7 1,743,129 - cysQ-2 SO1655 0.51 +0.3 1,743,338 - cysQ-2 SO1655 0.78 +2.8 1,743,422 + cysQ-2 SO1655 0.89 +1.7 1,743,428 + +1.6 1,743,430 - +1.2 1,743,565 - -2.2 1,743,640 + -1.0 1,743,690 - SO1656 0.14 +2.2 1,743,776 + SO1656 0.23 -1.3 1,743,776 + SO1656 0.23 -1.9 1,743,776 + SO1656 0.23 -2.9 1,743,776 + SO1656 0.23 -2.4 1,743,776 + SO1656 0.23 +3.5 1,743,784 - SO1656 0.24 -1.2 1,743,784 - SO1656 0.24 -1.3 1,743,784 - SO1656 0.24 -0.6 1,743,818 - SO1656 0.27 +0.2 1,743,820 + SO1656 0.28 +1.4 1,743,836 + SO1656 0.29 -1.1 1,743,855 - SO1656 0.31 -0.3 1,743,879 + SO1656 0.34 -0.1 1,743,976 - SO1656 0.45 -1.6 1,744,020 - SO1656 0.49 -2.3 1,744,073 - SO1656 0.55 -2.6 1,744,313 - SO1656 0.81 +1.0 1,744,327 - SO1656 0.83 -0.0 1,744,349 - SO1656 0.85 -0.8 1,744,463 - +1.3 1,744,473 - +1.7 1,744,557 + -0.2 1,744,557 + -0.2 1,744,623 - -1.3 1,744,790 - SO1657 0.28 +1.0 1,744,794 + SO1657 0.29 -1.7 1,744,802 - SO1657 0.30 -1.8 1,744,925 - SO1657 0.47 +0.9 1,745,006 - SO1657 0.58 +2.1 1,745,024 - SO1657 0.60 +0.4 1,745,359 + +1.5 1,745,378 + +2.2 1,745,378 + -1.8 1,745,378 + -1.5 1,745,386 - -0.1 1,745,386 - -1.0 1,745,386 - -0.6 1,745,386 - +1.7 1,745,386 - -0.0 1,745,464 - SO1658 0.20 +0.1 1,745,480 - SO1658 0.22 +1.0
Or see this region's nucleotide sequence