Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepA-1 and SO0960 are separated by 89 nucleotides SO0960 and SO0961 are separated by 183 nucleotides SO0961 and SO0962 are separated by 245 nucleotides
SO0959: pepA-1 - cytosol aminopeptidase (NCBI ptt file), at 993,944 to 995,479
pepA-1
SO0960: SO0960 - hypothetical spermidine synthase (NCBI ptt file), at 995,569 to 996,312
SO0960
SO0961: SO0961 - conserved hypothetical protein (NCBI ptt file), at 996,496 to 996,948
SO0961
SO0962: SO0962 - conserved hypothetical protein (NCBI ptt file), at 997,194 to 997,718
SO0962
Position (kb) 995
996
997 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 994.590 kb on + strand, within pepA-1 at 994.603 kb on + strand, within pepA-1 at 994.649 kb on + strand, within pepA-1 at 994.657 kb on - strand, within pepA-1 at 994.657 kb on - strand, within pepA-1 at 994.703 kb on - strand, within pepA-1 at 994.762 kb on + strand, within pepA-1 at 994.770 kb on - strand, within pepA-1 at 994.809 kb on - strand, within pepA-1 at 994.844 kb on + strand, within pepA-1 at 994.872 kb on + strand, within pepA-1 at 995.019 kb on - strand, within pepA-1 at 995.034 kb on - strand, within pepA-1 at 995.034 kb on - strand, within pepA-1 at 995.044 kb on + strand, within pepA-1 at 995.044 kb on + strand, within pepA-1 at 995.047 kb on - strand, within pepA-1 at 995.052 kb on - strand, within pepA-1 at 995.054 kb on + strand, within pepA-1 at 995.132 kb on + strand, within pepA-1 at 995.224 kb on + strand, within pepA-1 at 995.232 kb on - strand, within pepA-1 at 995.338 kb on + strand at 995.338 kb on + strand at 995.346 kb on + strand at 995.354 kb on - strand at 995.356 kb on + strand at 995.356 kb on + strand at 995.356 kb on + strand at 995.356 kb on + strand at 995.356 kb on + strand at 995.356 kb on + strand at 995.364 kb on - strand at 995.364 kb on - strand at 995.364 kb on - strand at 995.424 kb on + strand at 995.425 kb on + strand at 995.432 kb on - strand at 995.435 kb on + strand at 995.435 kb on + strand at 995.443 kb on - strand at 995.443 kb on - strand at 995.500 kb on - strand at 995.500 kb on - strand at 995.504 kb on + strand at 995.512 kb on - strand at 995.526 kb on + strand at 995.526 kb on + strand at 995.526 kb on + strand at 995.534 kb on - strand at 995.534 kb on - strand at 995.534 kb on - strand at 995.534 kb on - strand at 995.549 kb on - strand at 995.632 kb on + strand at 995.701 kb on + strand, within SO0960 at 995.709 kb on - strand, within SO0960 at 995.859 kb on + strand, within SO0960 at 995.875 kb on - strand, within SO0960 at 995.875 kb on - strand, within SO0960 at 995.887 kb on + strand, within SO0960 at 995.999 kb on + strand, within SO0960 at 996.072 kb on + strand, within SO0960 at 996.072 kb on + strand, within SO0960 at 996.080 kb on - strand, within SO0960 at 996.100 kb on + strand, within SO0960 at 996.116 kb on + strand, within SO0960 at 996.116 kb on + strand, within SO0960 at 996.154 kb on - strand, within SO0960 at 996.156 kb on + strand, within SO0960 at 996.159 kb on + strand, within SO0960 at 996.164 kb on - strand, within SO0960 at 996.164 kb on - strand, within SO0960 at 996.164 kb on - strand, within SO0960 at 996.227 kb on - strand, within SO0960 at 996.245 kb on + strand at 996.267 kb on + strand at 996.275 kb on - strand at 996.362 kb on - strand at 996.464 kb on + strand at 996.471 kb on - strand at 996.472 kb on - strand at 996.504 kb on + strand at 996.504 kb on + strand at 996.504 kb on + strand at 996.507 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.512 kb on - strand at 996.572 kb on + strand, within SO0961 at 996.587 kb on + strand, within SO0961 at 996.595 kb on - strand, within SO0961 at 996.602 kb on + strand, within SO0961 at 996.650 kb on - strand, within SO0961 at 996.668 kb on + strand, within SO0961 at 996.676 kb on - strand, within SO0961 at 996.676 kb on - strand, within SO0961 at 996.731 kb on - strand, within SO0961 at 996.731 kb on - strand, within SO0961 at 996.733 kb on - strand, within SO0961 at 996.758 kb on - strand, within SO0961 at 996.765 kb on - strand, within SO0961 at 996.824 kb on - strand, within SO0961 at 996.979 kb on - strand at 997.141 kb on - strand at 997.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 994,590 + pepA-1 SO0959 0.42 +1.0 994,603 + pepA-1 SO0959 0.43 +2.0 994,649 + pepA-1 SO0959 0.46 +1.5 994,657 - pepA-1 SO0959 0.46 +1.0 994,657 - pepA-1 SO0959 0.46 +1.3 994,703 - pepA-1 SO0959 0.49 +1.5 994,762 + pepA-1 SO0959 0.53 +0.4 994,770 - pepA-1 SO0959 0.54 +0.0 994,809 - pepA-1 SO0959 0.56 -0.1 994,844 + pepA-1 SO0959 0.59 +1.6 994,872 + pepA-1 SO0959 0.60 -1.2 995,019 - pepA-1 SO0959 0.70 +0.7 995,034 - pepA-1 SO0959 0.71 -0.1 995,034 - pepA-1 SO0959 0.71 +0.1 995,044 + pepA-1 SO0959 0.72 +1.5 995,044 + pepA-1 SO0959 0.72 -0.9 995,047 - pepA-1 SO0959 0.72 +0.5 995,052 - pepA-1 SO0959 0.72 +0.3 995,054 + pepA-1 SO0959 0.72 -0.8 995,132 + pepA-1 SO0959 0.77 -4.2 995,224 + pepA-1 SO0959 0.83 -0.9 995,232 - pepA-1 SO0959 0.84 +0.8 995,338 + +2.4 995,338 + +0.7 995,346 + +1.3 995,354 - -1.3 995,356 + -1.0 995,356 + -0.1 995,356 + +0.6 995,356 + +0.7 995,356 + -0.4 995,356 + -0.3 995,364 - +0.4 995,364 - +0.8 995,364 - -0.0 995,424 + +0.5 995,425 + +0.2 995,432 - -0.1 995,435 + -1.5 995,435 + -0.4 995,443 - -1.3 995,443 - +1.5 995,500 - +1.1 995,500 - -1.3 995,504 + -1.3 995,512 - +0.9 995,526 + +0.2 995,526 + -1.6 995,526 + +0.9 995,534 - -0.4 995,534 - +0.2 995,534 - +0.1 995,534 - -0.6 995,549 - -1.0 995,632 + -0.7 995,701 + SO0960 0.18 +1.7 995,709 - SO0960 0.19 +1.3 995,859 + SO0960 0.39 -1.0 995,875 - SO0960 0.41 -0.2 995,875 - SO0960 0.41 +1.1 995,887 + SO0960 0.43 +1.5 995,999 + SO0960 0.58 -2.3 996,072 + SO0960 0.68 +0.6 996,072 + SO0960 0.68 +1.0 996,080 - SO0960 0.69 +0.2 996,100 + SO0960 0.71 -0.7 996,116 + SO0960 0.74 +0.5 996,116 + SO0960 0.74 -0.8 996,154 - SO0960 0.79 -0.9 996,156 + SO0960 0.79 -0.4 996,159 + SO0960 0.79 -1.1 996,164 - SO0960 0.80 +0.9 996,164 - SO0960 0.80 +1.0 996,164 - SO0960 0.80 -0.1 996,227 - SO0960 0.88 +0.2 996,245 + -1.4 996,267 + +1.3 996,275 - -0.6 996,362 - -2.2 996,464 + -1.8 996,471 - +0.2 996,472 - -1.6 996,504 + -0.0 996,504 + -1.2 996,504 + +1.2 996,507 - -0.7 996,512 - -0.5 996,512 - +0.7 996,512 - -0.3 996,512 - +0.5 996,512 - +0.2 996,572 + SO0961 0.17 -1.1 996,587 + SO0961 0.20 -2.2 996,595 - SO0961 0.22 -0.1 996,602 + SO0961 0.23 -0.5 996,650 - SO0961 0.34 -0.5 996,668 + SO0961 0.38 -0.7 996,676 - SO0961 0.40 -1.5 996,676 - SO0961 0.40 +0.3 996,731 - SO0961 0.52 +0.7 996,731 - SO0961 0.52 -0.9 996,733 - SO0961 0.52 -0.8 996,758 - SO0961 0.58 +0.5 996,765 - SO0961 0.59 +0.5 996,824 - SO0961 0.72 +0.0 996,979 - +1.6 997,141 - -2.6 997,164 + +0.9
Or see this region's nucleotide sequence