Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prkB and SO0879 are separated by 84 nucleotides SO0879 and SO0880 are separated by 9 nucleotides SO0880 and SO0881 are separated by 111 nucleotides SO0881 and SO0882 are separated by 46 nucleotides
SO0878: prkB - phosphoribulokinase (NCBI ptt file), at 907,976 to 908,863
prkB
SO0879: SO0879 - conserved hypothetical protein (NCBI ptt file), at 908,948 to 909,193
SO0879
SO0880: SO0880 - conserved hypothetical protein (NCBI ptt file), at 909,203 to 910,186
SO0880
SO0881: SO0881 - conserved hypothetical protein (NCBI ptt file), at 910,298 to 910,804
SO0881
SO0882: SO0882 - oxidoreductase, GMC family (NCBI ptt file), at 910,851 to 912,452
SO0882
Position (kb) 909
910
911 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 908.216 kb on - strand, within prkB at 908.218 kb on + strand, within prkB at 908.299 kb on + strand, within prkB at 908.350 kb on + strand, within prkB at 908.372 kb on - strand, within prkB at 908.431 kb on + strand, within prkB at 908.455 kb on - strand, within prkB at 908.465 kb on + strand, within prkB at 908.473 kb on - strand, within prkB at 908.473 kb on - strand, within prkB at 908.473 kb on - strand, within prkB at 908.557 kb on + strand, within prkB at 908.623 kb on + strand, within prkB at 908.631 kb on - strand, within prkB at 908.641 kb on - strand, within prkB at 908.760 kb on - strand, within prkB at 908.762 kb on + strand, within prkB at 908.822 kb on + strand at 908.830 kb on - strand at 908.872 kb on + strand at 909.142 kb on - strand, within SO0879 at 909.308 kb on + strand, within SO0880 at 909.412 kb on + strand, within SO0880 at 909.592 kb on + strand, within SO0880 at 909.703 kb on + strand, within SO0880 at 909.799 kb on - strand, within SO0880 at 909.826 kb on + strand, within SO0880 at 909.826 kb on + strand, within SO0880 at 909.834 kb on - strand, within SO0880 at 909.892 kb on + strand, within SO0880 at 909.900 kb on - strand, within SO0880 at 909.910 kb on - strand, within SO0880 at 909.947 kb on + strand, within SO0880 at 909.955 kb on + strand, within SO0880 at 909.969 kb on + strand, within SO0880 at 910.083 kb on - strand, within SO0880 at 910.083 kb on - strand, within SO0880 at 910.083 kb on - strand, within SO0880 at 910.109 kb on - strand at 910.166 kb on - strand at 910.305 kb on + strand at 910.377 kb on + strand, within SO0881 at 910.377 kb on + strand, within SO0881 at 910.402 kb on + strand, within SO0881 at 910.545 kb on - strand, within SO0881 at 910.654 kb on + strand, within SO0881 at 910.752 kb on + strand, within SO0881 at 910.760 kb on + strand at 910.760 kb on - strand at 910.760 kb on - strand at 910.774 kb on + strand at 910.774 kb on + strand at 910.782 kb on - strand at 910.895 kb on + strand at 910.896 kb on + strand at 910.898 kb on + strand at 911.083 kb on + strand, within SO0882
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 908,216 - prkB SO0878 0.27 +0.8 908,218 + prkB SO0878 0.27 -1.2 908,299 + prkB SO0878 0.36 -1.0 908,350 + prkB SO0878 0.42 -1.2 908,372 - prkB SO0878 0.45 -0.4 908,431 + prkB SO0878 0.51 +0.3 908,455 - prkB SO0878 0.54 +0.1 908,465 + prkB SO0878 0.55 +0.1 908,473 - prkB SO0878 0.56 +1.4 908,473 - prkB SO0878 0.56 +0.9 908,473 - prkB SO0878 0.56 -0.1 908,557 + prkB SO0878 0.65 +0.9 908,623 + prkB SO0878 0.73 -0.3 908,631 - prkB SO0878 0.74 -0.6 908,641 - prkB SO0878 0.75 -0.8 908,760 - prkB SO0878 0.88 +0.4 908,762 + prkB SO0878 0.89 -1.6 908,822 + +0.8 908,830 - +0.3 908,872 + -0.1 909,142 - SO0879 0.79 +1.7 909,308 + SO0880 0.11 -1.3 909,412 + SO0880 0.21 +1.4 909,592 + SO0880 0.40 +1.0 909,703 + SO0880 0.51 -0.7 909,799 - SO0880 0.61 +1.3 909,826 + SO0880 0.63 -2.3 909,826 + SO0880 0.63 +0.1 909,834 - SO0880 0.64 -1.3 909,892 + SO0880 0.70 -1.9 909,900 - SO0880 0.71 +2.7 909,910 - SO0880 0.72 +0.5 909,947 + SO0880 0.76 -0.4 909,955 + SO0880 0.76 +1.4 909,969 + SO0880 0.78 +0.6 910,083 - SO0880 0.89 +0.9 910,083 - SO0880 0.89 -0.2 910,083 - SO0880 0.89 +0.0 910,109 - +1.5 910,166 - -0.3 910,305 + -3.5 910,377 + SO0881 0.16 -0.2 910,377 + SO0881 0.16 -0.6 910,402 + SO0881 0.21 +1.4 910,545 - SO0881 0.49 +0.8 910,654 + SO0881 0.70 -1.6 910,752 + SO0881 0.90 -1.4 910,760 + -0.9 910,760 - +1.4 910,760 - -4.0 910,774 + -1.1 910,774 + +1.6 910,782 - -3.5 910,895 + -3.0 910,896 + -1.5 910,898 + -0.2 911,083 + SO0882 0.14 -0.4
Or see this region's nucleotide sequence