Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amt and glnB-1 are separated by 34 nucleotides glnB-1 and SO0762 are separated by 308 nucleotides SO0762 and SO0763 are separated by 376 nucleotides SO0763 and SO0764 overlap by 14 nucleotides
SO0760: amt - ammonium transporter (NCBI ptt file), at 775,008 to 776,234
amt
SO0761: glnB-1 - nitrogen regulatory protein P-II 1 (NCBI ptt file), at 776,269 to 776,607
glnB-1
SO0762: SO0762 - isochorismatase family protein (NCBI ptt file), at 776,916 to 777,458
SO0762
SO0763: SO0763 - hypothetical protein (NCBI ptt file), at 777,835 to 778,056
SO0763
SO0764: SO0764 - hypothetical protein (NCBI ptt file), at 778,043 to 778,852
SO0764
Position (kb)
776
777
778 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 776.019 kb on - strand, within amt at 776.124 kb on + strand at 776.124 kb on + strand at 776.124 kb on + strand at 776.158 kb on + strand at 776.214 kb on + strand at 776.275 kb on - strand at 776.282 kb on + strand at 776.290 kb on - strand at 776.316 kb on + strand, within glnB-1 at 776.380 kb on - strand, within glnB-1 at 776.462 kb on + strand, within glnB-1 at 776.483 kb on - strand, within glnB-1 at 776.504 kb on + strand, within glnB-1 at 776.527 kb on + strand, within glnB-1 at 776.599 kb on - strand at 776.614 kb on - strand at 776.788 kb on - strand at 776.915 kb on - strand at 776.940 kb on - strand at 776.984 kb on - strand, within SO0762 at 777.028 kb on - strand, within SO0762 at 777.030 kb on + strand, within SO0762 at 777.053 kb on + strand, within SO0762 at 777.063 kb on + strand, within SO0762 at 777.156 kb on + strand, within SO0762 at 777.174 kb on - strand, within SO0762 at 777.319 kb on + strand, within SO0762 at 777.369 kb on + strand, within SO0762 at 777.384 kb on - strand, within SO0762 at 777.408 kb on + strand at 777.468 kb on + strand at 777.497 kb on + strand at 777.497 kb on + strand at 777.497 kb on + strand at 777.497 kb on + strand at 777.505 kb on - strand at 777.505 kb on - strand at 777.505 kb on - strand at 777.505 kb on - strand at 777.517 kb on - strand at 777.533 kb on - strand at 777.540 kb on + strand at 777.556 kb on + strand at 777.556 kb on + strand at 777.556 kb on + strand at 777.617 kb on - strand at 777.619 kb on - strand at 777.667 kb on - strand at 777.927 kb on - strand, within SO0763 at 777.927 kb on - strand, within SO0763 at 777.963 kb on + strand, within SO0763 at 777.963 kb on + strand, within SO0763 at 777.963 kb on + strand, within SO0763 at 777.971 kb on - strand, within SO0763 at 777.971 kb on - strand, within SO0763 at 777.971 kb on - strand, within SO0763 at 777.983 kb on - strand, within SO0763 at 778.021 kb on + strand, within SO0763 at 778.022 kb on + strand, within SO0763 at 778.030 kb on - strand, within SO0763 at 778.030 kb on - strand, within SO0763 at 778.050 kb on + strand at 778.058 kb on - strand at 778.106 kb on + strand at 778.120 kb on + strand at 778.140 kb on - strand, within SO0764 at 778.158 kb on - strand, within SO0764 at 778.233 kb on + strand, within SO0764 at 778.268 kb on - strand, within SO0764 at 778.275 kb on + strand, within SO0764 at 778.342 kb on + strand, within SO0764 at 778.342 kb on + strand, within SO0764 at 778.350 kb on - strand, within SO0764 at 778.423 kb on + strand, within SO0764 at 778.423 kb on + strand, within SO0764
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 776,019 - amt SO0760 0.82 +0.6 776,124 + -1.4 776,124 + +0.0 776,124 + -2.7 776,158 + -2.5 776,214 + -1.1 776,275 - +0.9 776,282 + -0.3 776,290 - -0.9 776,316 + glnB-1 SO0761 0.14 -2.1 776,380 - glnB-1 SO0761 0.33 -2.6 776,462 + glnB-1 SO0761 0.57 +0.6 776,483 - glnB-1 SO0761 0.63 -0.4 776,504 + glnB-1 SO0761 0.69 -1.9 776,527 + glnB-1 SO0761 0.76 +0.0 776,599 - -2.8 776,614 - +2.4 776,788 - -1.5 776,915 - -0.3 776,940 - +0.2 776,984 - SO0762 0.13 +1.2 777,028 - SO0762 0.21 -0.4 777,030 + SO0762 0.21 -0.4 777,053 + SO0762 0.25 +0.7 777,063 + SO0762 0.27 +1.9 777,156 + SO0762 0.44 +0.3 777,174 - SO0762 0.48 -0.2 777,319 + SO0762 0.74 +0.9 777,369 + SO0762 0.83 +1.2 777,384 - SO0762 0.86 +1.0 777,408 + -0.9 777,468 + +1.8 777,497 + -1.9 777,497 + +0.6 777,497 + -0.9 777,497 + +1.3 777,505 - +0.2 777,505 - -0.1 777,505 - +0.2 777,505 - +2.3 777,517 - -3.1 777,533 - +2.2 777,540 + +1.9 777,556 + -0.6 777,556 + -3.2 777,556 + -0.6 777,617 - -1.3 777,619 - +0.4 777,667 - -0.1 777,927 - SO0763 0.41 +0.1 777,927 - SO0763 0.41 +0.0 777,963 + SO0763 0.58 -0.4 777,963 + SO0763 0.58 -0.5 777,963 + SO0763 0.58 +1.0 777,971 - SO0763 0.61 -0.7 777,971 - SO0763 0.61 -0.9 777,971 - SO0763 0.61 +0.2 777,983 - SO0763 0.67 -0.5 778,021 + SO0763 0.84 -0.4 778,022 + SO0763 0.84 +0.8 778,030 - SO0763 0.88 -0.3 778,030 - SO0763 0.88 +2.5 778,050 + -2.1 778,058 - -1.4 778,106 + -0.4 778,120 + +1.3 778,140 - SO0764 0.12 +0.4 778,158 - SO0764 0.14 +2.0 778,233 + SO0764 0.23 -1.6 778,268 - SO0764 0.28 -1.6 778,275 + SO0764 0.29 +1.4 778,342 + SO0764 0.37 +2.9 778,342 + SO0764 0.37 +0.1 778,350 - SO0764 0.38 -1.6 778,423 + SO0764 0.47 -1.2 778,423 + SO0764 0.47 -1.3
Or see this region's nucleotide sequence