Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prmA and frdC are separated by 440 nucleotides frdC and fdrC are separated by 232 nucleotides fdrC and frdA are separated by 65 nucleotides
SO0395: prmA - ribosomal protein L11 methyltransferase (NCBI ptt file), at 408,364 to 409,245
prmA
SO0396: frdC - fumarate reductase cytochrome B subunit (NCBI ptt file), at 409,686 to 410,411
frdC
SO0397: fdrC - fumarate reductase cytochrome B subunit (NCBI ptt file), at 410,644 to 411,315
fdrC
SO0398: frdA - fumarate reductase flavoprotein subunit (NCBI ptt file), at 411,381 to 413,387
frdA
Position (kb) 409
410
411 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 408.749 kb on - strand, within prmA at 408.772 kb on + strand, within prmA at 408.777 kb on + strand, within prmA at 408.785 kb on - strand, within prmA at 408.785 kb on - strand, within prmA at 408.795 kb on + strand, within prmA at 408.795 kb on + strand, within prmA at 408.805 kb on - strand, within prmA at 408.812 kb on + strand, within prmA at 408.812 kb on + strand, within prmA at 408.812 kb on + strand, within prmA at 408.820 kb on - strand, within prmA at 408.820 kb on - strand, within prmA at 408.820 kb on - strand, within prmA at 408.820 kb on - strand, within prmA at 408.840 kb on - strand, within prmA at 408.866 kb on + strand, within prmA at 408.893 kb on - strand, within prmA at 408.895 kb on + strand, within prmA at 408.989 kb on - strand, within prmA at 409.022 kb on + strand, within prmA at 409.032 kb on + strand, within prmA at 409.032 kb on + strand, within prmA at 409.069 kb on - strand, within prmA at 409.143 kb on - strand, within prmA at 409.211 kb on + strand at 409.221 kb on - strand at 409.256 kb on - strand at 409.445 kb on - strand at 409.561 kb on + strand at 409.569 kb on - strand at 409.593 kb on + strand at 409.593 kb on + strand at 409.601 kb on - strand at 409.693 kb on + strand at 409.723 kb on - strand at 409.727 kb on + strand at 409.734 kb on + strand at 409.739 kb on + strand at 409.747 kb on - strand at 409.761 kb on + strand, within frdC at 409.780 kb on + strand, within frdC at 409.788 kb on - strand, within frdC at 409.860 kb on + strand, within frdC at 409.871 kb on + strand, within frdC at 409.894 kb on + strand, within frdC at 410.035 kb on + strand, within frdC at 410.035 kb on + strand, within frdC at 410.043 kb on - strand, within frdC at 410.043 kb on - strand, within frdC at 410.208 kb on - strand, within frdC at 410.208 kb on - strand, within frdC at 410.215 kb on + strand, within frdC at 410.217 kb on + strand, within frdC at 410.217 kb on + strand, within frdC at 410.220 kb on - strand, within frdC at 410.256 kb on - strand, within frdC at 410.264 kb on + strand, within frdC at 410.279 kb on + strand, within frdC at 410.279 kb on + strand, within frdC at 410.287 kb on - strand, within frdC at 410.289 kb on - strand, within frdC at 410.376 kb on - strand at 410.393 kb on + strand at 410.393 kb on + strand at 410.393 kb on + strand at 410.393 kb on + strand at 410.401 kb on - strand at 410.401 kb on - strand at 410.441 kb on + strand at 410.441 kb on + strand at 410.531 kb on + strand at 410.531 kb on + strand at 410.599 kb on - strand at 410.695 kb on - strand at 410.743 kb on + strand, within fdrC at 410.776 kb on + strand, within fdrC at 410.784 kb on - strand, within fdrC at 410.786 kb on + strand, within fdrC at 410.786 kb on + strand, within fdrC at 410.825 kb on - strand, within fdrC at 410.838 kb on + strand, within fdrC at 410.845 kb on + strand, within fdrC at 410.845 kb on + strand, within fdrC at 410.846 kb on - strand, within fdrC at 410.853 kb on - strand, within fdrC at 410.855 kb on + strand, within fdrC at 410.958 kb on - strand, within fdrC at 410.965 kb on + strand, within fdrC at 410.967 kb on + strand, within fdrC at 411.079 kb on - strand, within fdrC at 411.146 kb on + strand, within fdrC at 411.146 kb on + strand, within fdrC at 411.154 kb on - strand, within fdrC at 411.373 kb on + strand at 411.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 408,749 - prmA SO0395 0.44 +1.4 408,772 + prmA SO0395 0.46 +1.2 408,777 + prmA SO0395 0.47 -0.0 408,785 - prmA SO0395 0.48 -0.0 408,785 - prmA SO0395 0.48 -0.7 408,795 + prmA SO0395 0.49 -1.0 408,795 + prmA SO0395 0.49 -2.2 408,805 - prmA SO0395 0.50 -0.5 408,812 + prmA SO0395 0.51 -0.5 408,812 + prmA SO0395 0.51 -0.5 408,812 + prmA SO0395 0.51 -0.3 408,820 - prmA SO0395 0.52 -0.5 408,820 - prmA SO0395 0.52 +0.7 408,820 - prmA SO0395 0.52 -0.1 408,820 - prmA SO0395 0.52 -0.8 408,840 - prmA SO0395 0.54 -0.2 408,866 + prmA SO0395 0.57 -1.7 408,893 - prmA SO0395 0.60 +1.1 408,895 + prmA SO0395 0.60 +0.5 408,989 - prmA SO0395 0.71 -0.3 409,022 + prmA SO0395 0.75 -1.6 409,032 + prmA SO0395 0.76 +0.4 409,032 + prmA SO0395 0.76 +0.1 409,069 - prmA SO0395 0.80 +1.8 409,143 - prmA SO0395 0.88 -1.4 409,211 + +0.1 409,221 - -1.6 409,256 - -1.5 409,445 - -0.1 409,561 + -1.4 409,569 - -1.6 409,593 + +0.6 409,593 + -0.2 409,601 - -1.1 409,693 + -0.3 409,723 - +0.5 409,727 + +2.2 409,734 + +0.2 409,739 + -1.1 409,747 - -2.1 409,761 + frdC SO0396 0.10 +1.0 409,780 + frdC SO0396 0.13 +1.2 409,788 - frdC SO0396 0.14 +0.5 409,860 + frdC SO0396 0.24 +0.7 409,871 + frdC SO0396 0.25 +0.1 409,894 + frdC SO0396 0.29 +1.5 410,035 + frdC SO0396 0.48 +1.7 410,035 + frdC SO0396 0.48 +2.6 410,043 - frdC SO0396 0.49 +0.5 410,043 - frdC SO0396 0.49 -0.2 410,208 - frdC SO0396 0.72 -1.4 410,208 - frdC SO0396 0.72 -0.0 410,215 + frdC SO0396 0.73 +1.4 410,217 + frdC SO0396 0.73 -0.2 410,217 + frdC SO0396 0.73 +1.3 410,220 - frdC SO0396 0.74 +0.8 410,256 - frdC SO0396 0.79 -0.6 410,264 + frdC SO0396 0.80 +0.8 410,279 + frdC SO0396 0.82 +0.7 410,279 + frdC SO0396 0.82 +3.2 410,287 - frdC SO0396 0.83 -3.0 410,289 - frdC SO0396 0.83 +0.8 410,376 - +0.4 410,393 + +1.2 410,393 + +2.4 410,393 + +1.0 410,393 + -0.1 410,401 - -0.5 410,401 - -1.4 410,441 + +1.8 410,441 + +1.0 410,531 + +1.1 410,531 + +0.6 410,599 - +0.4 410,695 - -2.3 410,743 + fdrC SO0397 0.15 -1.2 410,776 + fdrC SO0397 0.20 +0.6 410,784 - fdrC SO0397 0.21 -0.8 410,786 + fdrC SO0397 0.21 +1.1 410,786 + fdrC SO0397 0.21 +0.3 410,825 - fdrC SO0397 0.27 +0.4 410,838 + fdrC SO0397 0.29 +1.6 410,845 + fdrC SO0397 0.30 +2.3 410,845 + fdrC SO0397 0.30 -0.3 410,846 - fdrC SO0397 0.30 +0.2 410,853 - fdrC SO0397 0.31 -0.1 410,855 + fdrC SO0397 0.31 +1.5 410,958 - fdrC SO0397 0.47 +0.8 410,965 + fdrC SO0397 0.48 -0.5 410,967 + fdrC SO0397 0.48 -1.1 411,079 - fdrC SO0397 0.65 -2.1 411,146 + fdrC SO0397 0.75 +0.1 411,146 + fdrC SO0397 0.75 +0.4 411,154 - fdrC SO0397 0.76 -1.6 411,373 + +1.4 411,397 - +0.3
Or see this region's nucleotide sequence