Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dam and SO0290 are separated by 32 nucleotides SO0290 and SO0291 overlap by 4 nucleotides SO0291 and rpe are separated by 262 nucleotides rpe and gph overlap by 1 nucleotides
SO0289: dam - DNA adenine methylase (NCBI ptt file), at 296,921 to 297,760
dam
SO0290: SO0290 - hypothetical protein (NCBI ptt file), at 297,793 to 297,981
SO0290
SO0291: SO0291 - hypothetical protein (NCBI ptt file), at 297,978 to 298,343
SO0291
SO0292: rpe - ribulose-phosphate 3-epimerase (NCBI ptt file), at 298,606 to 299,283
rpe
SO0293: gph - phosphoglycolate phosphatase (NCBI ptt file), at 299,283 to 299,966
gph
Position (kb) 297
298
299 Strain fitness (log2 ratio) -2
-1
0
1 at 297.924 kb on + strand, within SO0290 at 297.957 kb on - strand, within SO0290 at 297.997 kb on - strand at 298.029 kb on - strand, within SO0291 at 298.044 kb on - strand, within SO0291 at 298.054 kb on - strand, within SO0291 at 298.178 kb on - strand, within SO0291 at 298.187 kb on - strand, within SO0291 at 298.232 kb on - strand, within SO0291 at 298.500 kb on + strand at 298.540 kb on + strand at 299.000 kb on + strand, within rpe
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 297,924 + SO0290 0.69 +1.5 297,957 - SO0290 0.87 -0.9 297,997 - +0.7 298,029 - SO0291 0.14 -2.0 298,044 - SO0291 0.18 -0.4 298,054 - SO0291 0.21 -2.1 298,178 - SO0291 0.55 -0.2 298,187 - SO0291 0.57 +0.2 298,232 - SO0291 0.69 -0.2 298,500 + +0.3 298,540 + -1.2 299,000 + rpe SO0292 0.58 -1.5
Or see this region's nucleotide sequence