Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt selB and tRNA-SeC(p)-1 overlap by 125 nucleotides tRNA-SeC(p)-1 and fdhD are separated by 141 nucleotides fdhD and SO0108 are separated by 125 nucleotides
SO0106: selB - selenocysteine-specific translation elongation factor (NCBI ptt file), at 115,656 to 117,677
selB
SO_t002: tRNA-SeC(p)-1 - tRNA-Sec (RefSeq), at 117,553 to 117,643
tRNA-SeC(p)-1
SO0107: fdhD - formate dehydrogenase accessory protein FdhD (NCBI ptt file), at 117,785 to 118,621
fdhD
SO0108: SO0108 - hypothetical transporter component (NCBI ptt file), at 118,747 to 119,961
SO0108
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 116.790 kb on + strand, within selB at 116.832 kb on - strand, within selB at 117.006 kb on - strand, within selB at 117.026 kb on + strand, within selB at 117.081 kb on - strand, within selB at 117.117 kb on + strand, within selB at 117.134 kb on + strand, within selB at 117.152 kb on - strand, within selB at 117.159 kb on + strand, within selB at 117.274 kb on + strand, within selB at 117.274 kb on + strand, within selB at 117.274 kb on + strand, within selB at 117.282 kb on - strand, within selB at 117.317 kb on + strand, within selB at 117.349 kb on - strand, within selB at 117.394 kb on - strand, within selB at 117.400 kb on - strand, within selB at 117.407 kb on + strand, within selB at 117.430 kb on + strand, within selB at 117.576 kb on + strand at 117.576 kb on + strand at 117.576 kb on + strand at 117.576 kb on + strand at 117.584 kb on - strand at 117.601 kb on - strand at 117.628 kb on - strand at 117.679 kb on + strand at 117.895 kb on + strand, within fdhD at 117.901 kb on - strand, within fdhD at 117.970 kb on - strand, within fdhD at 118.039 kb on - strand, within fdhD at 118.080 kb on + strand, within fdhD at 118.119 kb on + strand, within fdhD at 118.127 kb on - strand, within fdhD at 118.147 kb on - strand, within fdhD at 118.152 kb on - strand, within fdhD at 118.228 kb on + strand, within fdhD at 118.248 kb on + strand, within fdhD at 118.256 kb on - strand, within fdhD at 118.256 kb on - strand, within fdhD at 118.256 kb on - strand, within fdhD at 118.292 kb on - strand, within fdhD at 118.311 kb on - strand, within fdhD at 118.328 kb on + strand, within fdhD at 118.333 kb on + strand, within fdhD at 118.430 kb on + strand, within fdhD at 118.519 kb on + strand, within fdhD at 118.526 kb on - strand, within fdhD at 118.564 kb on + strand at 118.635 kb on - strand at 118.644 kb on - strand at 118.865 kb on - strand at 118.947 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.969 kb on + strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 118.977 kb on - strand, within SO0108 at 119.063 kb on - strand, within SO0108 at 119.069 kb on + strand, within SO0108 at 119.083 kb on + strand, within SO0108 at 119.096 kb on - strand, within SO0108 at 119.137 kb on + strand, within SO0108 at 119.394 kb on - strand, within SO0108 at 119.399 kb on + strand, within SO0108 at 119.416 kb on + strand, within SO0108 at 119.417 kb on - strand, within SO0108 at 119.424 kb on - strand, within SO0108 at 119.429 kb on - strand, within SO0108 at 119.443 kb on - strand, within SO0108 at 119.458 kb on + strand, within SO0108 at 119.499 kb on - strand, within SO0108 at 119.501 kb on - strand, within SO0108 at 119.512 kb on + strand, within SO0108 at 119.606 kb on - strand, within SO0108
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 116,790 + selB SO0106 0.56 -2.8 116,832 - selB SO0106 0.58 -0.2 117,006 - selB SO0106 0.67 +2.4 117,026 + selB SO0106 0.68 -1.7 117,081 - selB SO0106 0.70 +0.7 117,117 + selB SO0106 0.72 +1.1 117,134 + selB SO0106 0.73 -0.3 117,152 - selB SO0106 0.74 +1.9 117,159 + selB SO0106 0.74 +1.3 117,274 + selB SO0106 0.80 -1.2 117,274 + selB SO0106 0.80 -0.6 117,274 + selB SO0106 0.80 -1.7 117,282 - selB SO0106 0.80 -2.2 117,317 + selB SO0106 0.82 -2.9 117,349 - selB SO0106 0.84 +0.9 117,394 - selB SO0106 0.86 +0.3 117,400 - selB SO0106 0.86 +2.0 117,407 + selB SO0106 0.87 -0.5 117,430 + selB SO0106 0.88 +0.5 117,576 + +1.1 117,576 + +0.3 117,576 + +1.0 117,576 + -0.8 117,584 - -2.4 117,601 - +0.7 117,628 - -1.3 117,679 + -1.9 117,895 + fdhD SO0107 0.13 -1.6 117,901 - fdhD SO0107 0.14 -3.1 117,970 - fdhD SO0107 0.22 -0.6 118,039 - fdhD SO0107 0.30 +0.4 118,080 + fdhD SO0107 0.35 -2.1 118,119 + fdhD SO0107 0.40 -2.0 118,127 - fdhD SO0107 0.41 -1.8 118,147 - fdhD SO0107 0.43 -0.9 118,152 - fdhD SO0107 0.44 -0.1 118,228 + fdhD SO0107 0.53 -1.0 118,248 + fdhD SO0107 0.55 -0.4 118,256 - fdhD SO0107 0.56 -0.9 118,256 - fdhD SO0107 0.56 -0.4 118,256 - fdhD SO0107 0.56 -1.4 118,292 - fdhD SO0107 0.61 +0.9 118,311 - fdhD SO0107 0.63 -1.1 118,328 + fdhD SO0107 0.65 -1.0 118,333 + fdhD SO0107 0.65 -0.1 118,430 + fdhD SO0107 0.77 -1.6 118,519 + fdhD SO0107 0.88 +0.0 118,526 - fdhD SO0107 0.89 -1.8 118,564 + +1.3 118,635 - +1.4 118,644 - +1.8 118,865 - +0.3 118,947 + SO0108 0.16 -3.4 118,969 + SO0108 0.18 -1.1 118,969 + SO0108 0.18 +2.6 118,969 + SO0108 0.18 -0.6 118,969 + SO0108 0.18 -0.8 118,969 + SO0108 0.18 -3.3 118,969 + SO0108 0.18 +1.1 118,969 + SO0108 0.18 +0.5 118,969 + SO0108 0.18 -0.3 118,969 + SO0108 0.18 -0.7 118,969 + SO0108 0.18 -2.2 118,969 + SO0108 0.18 -0.4 118,977 - SO0108 0.19 +2.0 118,977 - SO0108 0.19 -1.4 118,977 - SO0108 0.19 -0.9 118,977 - SO0108 0.19 +0.1 118,977 - SO0108 0.19 -0.1 118,977 - SO0108 0.19 -0.1 118,977 - SO0108 0.19 +1.6 118,977 - SO0108 0.19 +0.5 118,977 - SO0108 0.19 +0.5 119,063 - SO0108 0.26 +0.7 119,069 + SO0108 0.27 +1.2 119,083 + SO0108 0.28 -0.1 119,096 - SO0108 0.29 +1.5 119,137 + SO0108 0.32 -1.4 119,394 - SO0108 0.53 +1.6 119,399 + SO0108 0.54 -0.6 119,416 + SO0108 0.55 +0.3 119,417 - SO0108 0.55 -0.5 119,424 - SO0108 0.56 +1.1 119,429 - SO0108 0.56 -0.7 119,443 - SO0108 0.57 +0.8 119,458 + SO0108 0.59 -1.6 119,499 - SO0108 0.62 -1.8 119,501 - SO0108 0.62 +0.7 119,512 + SO0108 0.63 +2.3 119,606 - SO0108 0.71 -0.6
Or see this region's nucleotide sequence